Cerca de 90% dos tumores testiculares acontecem em caes com idades entre oito e dez anos quando comparado aos demais animais domesticos. A criptorquidia, condicao em que os testiculos nao descem da cavidade abdominal para a bolsa escrotal, e uma das principais causas, com incidencia de 10 a 14 vezes maior de desenvolver neoplasias. Os tumores testiculares primarios mais frequentes em caes sao Seminoma, Sertolioma e Tumor de celulas intersticiais, tambem denominado de Leydigocitoma. A maioria desses tumores tem comportamento benigno, e a proporcao de matriz extracelular e variavel nesses processos neoplasicos. O objetivo do estudo foi caracterizar com o metodo de HE e Tricromico de Picrosirius associado a polarizacao, o tipo de colageno presente nas tres neoplasias testiculares. Nas amostras analisadas, pode-se observar que ha predominio de colageno tipo I que envolve de forma descontinua as massas neoplasicas, entretanto, por entre as celulas neoplasicas nao ficou evidenciado nenhum tipo de colágeno.
Fibrosarcoma is characterized as an invasive nodular tumor of mesodermal origin, with elongated normochromatic nuclei. The histopathologic analysis using specific staining techniques is efficient in detailing the neoplastic architecture and the relations with surrounding tissues. In the present study, the canine skin fibrosarcoma was histologically characterized using Hematoxilin-Eosin stain, Mallory’s Trichrome stain and Shorr’s Trichrome stain as routine staining for conjunctive tissue, Toluidine Blue to highlight the mitosis and Picrosirius-Hematoxilin Trichrome to characterize the collagen type. It was concluded that the Picrosirius stain characterized the existing collagen type as type I and III; the Toluidine Blue staining highlighted the mitoses together with Shorr’s staining, as this one also provided a marking for the conjunctive tissue and the formed bands. The Mallory’s staining technique highlighted the conjunctive tissue and the bands that are spread; the Hematoxilin-Eosin stain highlighted with emphasis the neoplastic cells.
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