Histomonas meleagridis: Effect of gamma radiation on in vitro growth

High yield of parasites and prolongedin vitroculture ofHistomonas meleagridis

“…Initially a combination of a solid and a liquid medium was attempted (Drbohlav, 1924;Tyzzer, 1936), but later completely liquid culture media were developed (DeVolt, 1943). These usually consisted of buffered saline with 5 to 10% serum (of various mammal species) and other additions, including rice powder (Tyzzer, 1934(Tyzzer, , 1936Bishop, 1938;DeVolt, 1943;Delappe 1953;McDougald & Reid, 1978), marmite (Lucas & Goose, 1965), turkey caecal bacteria (Lesser, 1960(Lesser, , 1964Lund et al ., 1967), cream (Lesser, 1963(Lesser, , 1964, granular charcoal (Ruff & Hansen, 1970;Hirsch & Hirsch, 1977) and chicken embryo extract (Dwyer, 1970;Stepkowski & Klimont, 1979). A frequently used culture medium is Medium 199 with Hank's salts, 10% heat-inactivated horse serum, 5% chicken embryo extract and 10 to 12 mg rice powder per 12.5 ml medium (Dwyer, 1970;McDougald & Reid, 1978).…”