Leishmania enriettii: Radiation effects and evaluation of radioattenuated organisms for vaccination

Lemma, Alemayehu; Cole, Leonard J.

doi:10.1016/0014-4894(74)90019-8

Cited by 17 publications

(6 citation statements)

References 9 publications

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“…37, 1982 on August 16, 2020 by guest http://iai.asm.org/ Downloaded from immunoprophylaxis. It seems clear, however, that such an approach would likely necessitate the use of appropriate adjuvants, since killed or attenuated parasite preparations alone provide limited or no measurable protection in experimental hosts (4,16,17,20,21).…”

Section: Resultsmentioning

confidence: 99%

Protective effect of glucan against visceral leishmaniasis in hamsters

Cook¹,

Holbrook²,

Dougherty³

1982

Infect Immun

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The effect of pre- or posttreatment with glucan, a reticuloendothelial stimulant, on the course of Leishmania donovani infection was assessed in highly susceptible hamsters. Intravenous administration of glucan before or after L. donovani infection significantly suppressed proliferation of amastigote-stage parasites in liver and spleen. Glucan-activated peritoneal macrophages in vitro also significantly reduced multiplication of the intracellular parasite. Ultrastructural studies revealed a well-defined hepatic granulomatous response to glucan, with hypertrophic Kupffer cells and reduced numbers of intracellular parasites compared to the control group. In additional studies, groups of hamsters were immunized by intravenous injections of glucan with Formalin-killed promastigote-stage L. donovani cells and challenged 60 days after the last immunizing injection. This treatment regimen significantly prolonged the mean survival time of those hamsters which died after infection, relative to untreated control groups. Hamsters stimulated with the glucan-killed promastigote preparation also exhibited significant reductions in splenic amastigotes on days 10 and 21 postinfection compared with all other control groups, but on day 35, splenic amastigotes did not differ significantly from those of control animals. Our composite observations provide evidence for glucan-enhanced nonspecific resistance of hamsters to visceral leishmaniasis.

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Section: Resultsmentioning

confidence: 99%

Protective effect of glucan against visceral leishmaniasis in hamsters

Cook¹,

Holbrook²,

Dougherty³

1982

Infect Immun

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“…Use of γ-irradiated live attenuated Leishmania in treatment of leishmaniasis has long been mentioned by different research groups (Alexander 1982;Howard et al 1982Howard et al , 1984Lemma and Cole 1974;Rivier et al 1993Rivier et al , 1999. Live attenuated vaccine is a successful practice against smallpox, measles, mumps, and rubella, among others.…”

Section: Introductionmentioning

confidence: 97%

Therapeutic immunization with radio-attenuated Leishmania parasites through i.m. route revealed protection against the experimental murine visceral leishmaniasis

Datta¹,

Manna²,

Khanra³

et al. 2012

Parasitol Res

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“…Immunization trials with killed or attenuated Leishmania parasites have also been attempted in laboratory animals with variable results. A loss of infectivity was induced in L. enriettii by gamma radiation, but multiple vaccinations conferred no protection against the homologous parasite in guinea pigs (15). On the other hand, Coutinho (4) found that killed promastigotes of L. enriettii provided a limited degree of protection in guinea pigs.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity of soluble and particulate antigens from Leishmania donovani: effect of glucan as an adjuvant

Cook¹,

Holbrook²

1983

Infect Immun

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The protective efficacy of glucan as an adjuvant with killed promastigotes of Leishmania donovani was compared with that of soluble or particulate fractions of the parasite. When these vaccine preparations were injected either intravenously or subcutaneously in CF-1 mice, glucan potentiated resistance against L. donovani infections as reflected by significant reductions in hepatic amastigote counts relative to infected control mice. The leishmanial antigens alone afforded no protection. Serum direct agglutination titers to leishmanial antigens were highest in all groups given the vaccine intravenously, whereas the delayed-type hypersensitivity response to the antigen was positive only in groups immunized subcutaneously with glucan as an adjuvant. Some index of protection and immune response against visceral infection with the parasite was seen in groups vaccinated with glucan and soluble antigens. However, the protection afforded by glucan and particulate antigens of L. donovani more closely paralleled the resistance of mice treated with glucan and unfractionated killed promastigotes. Further antigenic analysis of particulate fractions of L. donovani may optimize effective immunization when used with appropriate adjuvants, e.g., glucan.

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Leishmania enriettii: Radiation effects and evaluation of radioattenuated organisms for vaccination

Cited by 17 publications

References 9 publications

Protective effect of glucan against visceral leishmaniasis in hamsters

Protective effect of glucan against visceral leishmaniasis in hamsters

Therapeutic immunization with radio-attenuated Leishmania parasites through i.m. route revealed protection against the experimental murine visceral leishmaniasis

Immunogenicity of soluble and particulate antigens from Leishmania donovani: effect of glucan as an adjuvant

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