High yield of parasites and prolonged<i>in vitro</i>culture of<i>Histomonas meleagridis</i>

Heijden, H.M.J.F. van der; McDougald, L. R.; Landman, W. J. M.

doi:10.1080/03079450500368474

Cited by 23 publications

(12 citation statements)

References 19 publications

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“…meleagridis isolates. An isolation procedure for H. meleagridis as described in more detail elsewhere (Van der Heijden et al ., 2005) was applied to birds from several sources. Briefly, immediately after euthanasia, caeca were opened and caecal fluid or content was inoculated into Dwyer's medium and incubated at 408C.…”

Section: Methodsmentioning

confidence: 99%

Genotyping ofHistomonas meleagridisisolates based on Internal Transcribed Spacer-1 sequences

Heijden¹,

Landman²,

Greve³

et al. 2006

Avian Pathology

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Section: Methodsmentioning

confidence: 99%

Genotyping ofHistomonas meleagridisisolates based on Internal Transcribed Spacer-1 sequences

Heijden¹,

Landman²,

Greve³

et al. 2006

Avian Pathology

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“…However, recent in-vitro studies clearly showed that Histomonas meleagridis only appears in two different shapes: an amoebic stage without a flagellum and a stage with a single flagellum (Hess et al 2005;Van der Heijden and Mc Dougald 2005;Grabensteiner et al 2007;Lotfi and Hafez 2008;Mielewczik et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Light and transmission electron microscopic studies on trophozoites and cyst-like stages of Histomonas meleagridis from cultures

et al. 2008

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The present study deals with Berlin strains of Histomonas meleagridis, the specimens of which were cultivated in Dwyer's medium. The light and electron microscopic examination revealed that the cultivated trophozoite stages (reaching about 10 mum in size) appeared more or less spherical, although their surface (covered by a single membrane) showed amoeba-like waves. All stages were uni-nucleated and reproduced by binary fission with an extranuclear spindle apparatus. Some trophozoites appeared ovoid and possessed a single flagellum with a typical microtubular 9 x 2 + 2 arrangement. Furthermore, the latter were characterized by an inner row of typical microtubules (remnant of an axostyle) and a Golgi apparatus (both adjacent to the nucleus), multivesicular structures, hydrogenosomes, and many food vacuoles containing either starch grains or bacteria. Their cytoplasm was densely filled with glycogen granules and ribosomes. Similar stages were also documented in the caeca and cloaca of chicken when being inoculated (via cloaca) with such culture stages. In addition to these typical trophozoites, the cultures contained a low number of 10-mum-sized spherical cyst-like stages with a surrounding amorphous layer. The cytoplasm of some of these cyst-like stages-when studied by electron microscopy-appeared with two membranes or had formed an amorphic, cyst-wall-like layer at their surface, apparently corresponding to their light microscopical appearance. Such stages might be involved in transmission from one host to another and probably have been missed before in microscopical examinations of infected poultry.

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“…using PCR as described by Grabensteiner and Hess (2006). Each bird received 1 ml modified Dwyers medium ( Van der Heijden et al 2005) containing 300,000 histomonads intracloacally. All birds were observed for 4 weeks after infection and daily mortality was recorded.…”

Section: Methodsmentioning

confidence: 99%

Susceptibility of different turkey lines to Histomonas meleagridis after experimental infection

Lotfi

Hafez

2009

Parasitol Res

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In the present investigation, three turkey lines, namely wild Canadian turkeys (WCT), British United turkey (BUT-Big6) and Kelly-Bronze turkeys (KBT) were compared for their susceptibility to infection with Histomonas meleagridis. All birds were kept on wood shaving as litter from day 1 on during the entire observation period. On day 28, 18-20 birds per turkey line were infected with H. meleagridis intracloacally. All birds were observed for 4 weeks after infection. The mortality rate was 95% in WCT, 78% in BUT-Big6 and 75% in KBT. In WCT, the first deaths occurred at day 6 and ended at day 13 post-infection, whilst for BUT-Big6 and KBT, birds died from days 10 to day 20. In KBT group, the mortality started at day 10 and lasted until day 17 after infection. At necropsy, all birds that died showed lesions typical for histomoniasis in the caeca and liver. The obtained results demonstrate that all tested turkey lines are susceptible to infection; however, the mortality rate for the wild Canadian turkey is statistically significantly higher compared to the other tested two lines.

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High yield of parasites and prolongedin vitroculture ofHistomonas meleagridis

Cited by 23 publications

References 19 publications

Genotyping ofHistomonas meleagridisisolates based on Internal Transcribed Spacer-1 sequences

Genotyping ofHistomonas meleagridisisolates based on Internal Transcribed Spacer-1 sequences

Light and transmission electron microscopic studies on trophozoites and cyst-like stages of Histomonas meleagridis from cultures

Susceptibility of different turkey lines to Histomonas meleagridis after experimental infection

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