Histology of the Tympanic Perforation and the Replacement Membrane

Abstract

This study shows that the mucocutaneous junction of a perforation of the tympanic membrane may be located at the margin, in the middle ear, or in the ear canal. It is incumbent upon the surgeon performing myringoplasty to identify this junction in order to optimize the success of surgery. About one-third of the healed area following perforation has an intermediate fibrous layer. The membranes in 2 cases of myringoplasty evidenced thicker-than-normal intermediate layers.

Basic Fibroblast Growth Factor Combined With Atelocollagen for Closing Chronic Tympanic Membrane Perforations in 87 Patients

“…Basic fibroblast growth factor also stimulates protease production by the granulation tissue, leading to increased matrix remodeling (17). These results may be clinically valuable because examinations of TM scars in humans have revealed the absence of a lamina propria and no arrangement of the fibers (18). Experimental studies have indicated that bFGF is produced in situ after laceration of the TM and facilitates healing of the perforation by stimulating the proliferation and differentiation of endothelial cells, fibroblasts, and keratinocytes at the margin.…”

Tympanic Membrane Perforation Repair with Acellular Porcine Submucosa

“…Multiple studies have demonstrated the results of various materials in the repair of tympanic membrane perforations (1)(2)(3)(4)(5)(6)10). The normal anatomy of the human and chinchilla TM consists of a thin lateral squamous epithelium, a middle fibrous layer, and a medial cuboidal mucosal layer.…”

Ein verbessertes Tiermodell f�r chronische Trommelfellperforationen

“…Träger der Reparation ist das äußere Epithel in Interaktion mit der Lamina propria [30], die Mukosa scheint von untergeordneter Bedeutung zu sein [19]. Vermittelt durch eine reparative Entzündung in der Lamina propria erfolgt eine Epithelmigration in Richtung des Defektzentrums, gefolgt von einer variablen Aussprossung des Bindegewebes [12,34]. Besonders aktive Epithelzonen liegen in Nähe des Hammergriffes und des Anulus.…”