Histologische Untersuchungen des Trommelfelldefektrandes bei der chronischen Otitis media mesotympanalis

Begall, K.; Dörschner, M.; Vorwerk, W.; Vorwerk, Ulrich; Dietzmann, Knut

doi:10.1007/s001060050305

Cited by 4 publications

(2 citation statements)

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“…Der histologische Befund von bis auf die Rückfläche des Perforationsrandes reichenden Keratinozyten findet sich auch bei der chronischen Trommelfellperforation des Menschen und gilt als ein Grund für eine Störung der Heilungsvorgänge [3]. Damit erweist sich das vorgestellte Tiermodell als eine geeignete Grundlage für systematische Untersuchungen der Reparation von chronischen Trommelfelldefekten.…”

Section: Mitomycinapplikationunclassified

Ein verbessertes Tiermodell f�r chronische Trommelfellperforationen

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Hosemann

Beule

et al. 2004

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Section: Mitomycinapplikationunclassified

Ein verbessertes Tiermodell f�r chronische Trommelfellperforationen

Kaftan

Hosemann

Beule

et al. 2004

HNO

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“…Therefore, the circular edge of the perforation epithelializes and interrupts defect closure (shown in Fig. 1) [2,13,[18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

The Dorsal Skinfold Chamber as a New Tympanic Membrane Wound Healing Model: Intravital Insights into the Pathophysiology of Epithelialized Wounds

et al. 2021

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Background: Tympanic membrane perforations (TMPs) are a common complication of trauma and infection. Persisting perforations result from the unique location of the tympanic membrane. The wound is surrounded by air of the middle ear and the external auditory canal. The inadequate wound bed, growth factor, and blood supply lead to circular epithelialization of the perforation’s edge and premature interruption of defect closure. Orthotopic animal models use mechanical or chemical tympanic membrane laceration to identify bioactive wound dressings and overcome premature epithelialization. However, all orthotopic models essentially lack repetitive visualization of the biomaterial-wound interface. Therefore, recent progress in 3D printing of customized wound dressings has not yet been transferred to the unique wound setup of the TMP. Here, we present a novel application for the mice dorsal skinfold chamber (DSC) with an epithelialized full-thickness defect as TMP model. Methods: A circular 2-mm defect was cut into the extended dorsal skinfold using a biopsy punch. The skinfold was either perforated through both skin layers without prior preparation or perforated on 1 side, following resection of the opposing skin layer. In both groups, the wound was sealed with a coverslip or left unclosed (n = 4). All animals were examined for epithelialization of the edge (histology), size of the perforation (planimetry), neovascularization (repetitive intravital fluorescence microscopy), and inflammation (immunohistology). Results: The edge of the perforation was overgrown by the cornified squamous epithelium in all preparations. Reduction in the perforation’s size was enhanced by application of a coverslip. Microsurgical preparation before biopsy punch perforation and sealing with a coverslip enabled repetitive high-quality intravital fluorescence microscopy. However, spontaneous reduction of the perforation occurred frequently. Therefore, the direct biopsy punch perforation without microsurgical preparation was favorable: spontaneous reduction did not occur throughout 21 days. Moreover, the visualization of the neovascularization was sufficient in intravital microscopy. Conclusions: The DSC full-thickness defect is a valuable supplement to orthotopic TMP models. Repetitive intravital microscopy of the epithelialized edge enables investigation of the underlying pathophysiology during the transition from the inflammation to the proliferation phase of wound healing. Using established analysis procedures, the present model provides an effective platform for the screening of bioactive materials and transferring progress in tissue engineering to the special conditions of tympanic membrane wound healing.

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