Histological response to platelet-rich plasma added to polypropylene mesh implemented in rabbits

Abstract

Introduction:The platelet-rich plasma (PRP) is part of a set of biotechnologies, providing some growth factors that promote repair of different tissues. The polypropylene meshes (PPM) are applied in the correction of abdominal defects, pelvic floor and urinary incontinence, however, they induce many significant complications, as a result of an inappropriate inflammatory response.Purpose:To investigate the changes caused by PRP associated with the implantation of PPM in the abdomen of female rabbits, in the production of collagen I and III and the inflammatory infiltrate (ININ).Materials and Methods:We performed implant meshes with and without PRP in adult rabbits (n=30) and euthanasia at 7, 30 and 90 days. Two plates were prepared from each animal and analyzed in five different fields. The ININ was evaluated by quantification of inflammatory cells using hematoxylin-eosin and the collagen by Sirius red method. The results were analyzed applying the Wilcoxon, Kruskal-Wallis, Junckheere and Friedmann tests.Results:There was a significant difference in the number of inflammatory cells between the groups with and without PRP (p=0.01) at 90 days. There was increased production of collagen I, III and total with the use of PRP, at seven days.Conclusion:The PPM coating with PRP was associated with increased ININ at the implant area, and an increasing trend during the process of tissue repair. The PPM coated with PRP was related to increased concentration of collagen I, collagen III and the concentration of total collagen increased after seven days of implantation.

Estimation of the Effect of Platelet Rich Plasma Products in the Integration of Polypropylene Mesh Implant in Biological Tissues. Experimental Model in Rats

“…In compare to earlier published data, which were modelled similar tissue reactions in rabbits -OscarRubiniÁvila, 2016 [22], the data obtained now correlate with the author's determined increase in the number of inflammatory cells in the study group. It should be noted that as a target for PRP in the experiment the actual fatty tissue of rabbits performed.…”

The importance of developing relevant animal models to assess existing and new materials

“…Recent Cochrane reviews on chronic wound healing and musculoskeletal soft tissue injuries question the level of evidence currently available [45,46]. Avila et al [47] compared in the rabbit the host response to subcutaneously inserted polypropylene, with or without PRP. PRP induced more inflammatory cells at 90 days, and collagens I and III as well as total collagen after 7 days [47].…”

“…Avila et al [47] compared in the rabbit the host response to subcutaneously inserted polypropylene, with or without PRP. PRP induced more inflammatory cells at 90 days, and collagens I and III as well as total collagen after 7 days [47]. Later on the same group (Parizzi et al [47]) did similar experiments by inserting a macroporous polypropylene, again with or without PRP, in the rectovaginal septum without fixation.…”

Can platelet-rich plasma coating improve polypropylene mesh integration? An immunohistochemical analysis in rabbits

“…Rabbits have a large vaginal cavity with bacterial flora similar to the human vagina (19). In a previous study conducted by our research group using rabbits, the effect of PRP added to PP meshes was associated with increased concentrations of collagen I and III seven days postoperatively (20).…”