Histological features of the spleen and pancreas of the Egyptian Nile monitor lizard (Varanus niloticus)

Mohammed, Elsayed S. I.; Madkour, Fatma A.; Radey, Rasha

doi:10.21608/svu.2021.96063.1149

Cited by 2 publications

(3 citation statements)

References 27 publications

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“…Interestingly, pancreatic islets were not found within the splenic lobe in the pancreas of the adult Nile monitor lizard Varanus niloticus (Mohammed et al., 2021) and water monitor lizard Varanus salvator (Hamny et al., 2016). Still, the islets were found in the duodenal lobe (Mohammed et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

Does the pancreas of gekkotans differentiate similarly? Developmental structural and 3D studies of the mourning gecko (Lepidodactylus lugubris) and the leopard gecko (Eublepharis macularius)

Kowalska,

Kaczmarek,

Rupik

2024

Journal of Anatomy

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This study investigated the pancreas differentiation of two species of gekkotan families—the mourning gecko Lepidodactylus lugubris (Gekkonidae) and the leopard gecko Eublepharis macularius (Eublepharidae)—based on two‐dimensional (2D) histological samples and three‐dimensional (3D) reconstructions of the position of the pancreatic buds and the surrounding organs. The results showed that at the moment of egg laying, the pancreas of L. lugubris is composed of three distinct primordia: one dorsal and two ventral. The dorsal primordium differentiates earlier than either ventral primordium. The right ventral primordium is more prominent and distinctive, starting to form earlier than the left one. Moreover, at this time, the pancreas of the leopard gecko is composed of the dorsal and right ventral primordium and the duct of the left ventral primordium. It means that the leopard gecko's left primordium is a transitional structure. These results indicate that the early development of the gekkotan pancreas is species specific. The pancreatic buds of the leopard and mourning gecko initially enter the duodenum by separate outlets, similar to the pancreas of other vertebrates. The pancreatic buds (3 of the mourning gecko and 2 of the leopard gecko) fuse quickly and form an embryonic pancreas. After that, the structure of this organ changes. After fusion, the pancreas of both gekkotans comprises four parts: the head of the pancreas (central region) and three lobes: upper, splenic, and lower. This organ develops gradually and is very well distinguished at hatching time. In both gekkotan species, cystic, hepatic, and pancreatic ducts enter the duodenum within the papilla. During gekkotan pancreas differentiation, the connection between the common bile duct and the dorsal pancreatic duct is associated with intestinal rotation, similar to other vertebrates.

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Section: Discussionmentioning

confidence: 99%

Does the pancreas of gekkotans differentiate similarly? Developmental structural and 3D studies of the mourning gecko (Lepidodactylus lugubris) and the leopard gecko (Eublepharis macularius)

Kowalska,

Kaczmarek,

Rupik

2024

Journal of Anatomy

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“…Modern reptiles have a digestive system that is structurally comparable to that of all higher vertebrates, as it consists of the buccal cavity with its associated structures, the esophagus, the stomach, the small and large intestine, as well as the accessory digestive glands as the liver and the pancreas (Kardong, 2012; Zaher et al, 2012;Srichairat et al, 2022). Since reptiles respond to food even better than other regularly used experimental mammals like mice, rats, rabbits, and pigs, they have been suggested to be a suitable model for investigating the physiological regulation of the digestive process in the future (Mohammed et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…During its foraging activity, it traverses quite a huge area (Ibrahim, 2010;Soliman and Mohallal, 2016).Modern reptiles have a digestive system that is structurally comparable to that of all higher vertebrates, as it consists of the buccal cavity with its associated structures, the esophagus, the stomach, the small and large intestine, as well as the accessory digestive glands as the liver and the pancreas (Kardong, 2012; Zaher et al, 2012;Srichairat et al, 2022). Since reptiles respond to food even better than other regularly used experimental mammals like mice, rats, rabbits, and pigs, they have been suggested to be a suitable model for investigating the physiological regulation of the digestive process in the future (Mohammed et al, 2021).Compared with mammals, reptiles possess a variety of peculiarities that could be of specific advantages for some histological and histochemical aspects (Zaher et al, 2012). In reptiles, the liver is the largest extrinsic digestive gland where different substances are taken in by intestinal capillaries and carried into the hepatic portal vein by tributaries, where the first processing.…”

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confidence: 99%

Melanomacrophage Centers: Comparative Histological Insights among Three Reptilian Species

Ahmed

2022

Egyptian Academic Journal of Biological Sciences, B. Zoology

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Background:Reptiles have been suggested to be a suitable model for the histological and histochemical aspects even better than other used experimental mammals. Melanomacrophage centers (MMCs) are aggregations of macrophage-like cells and pigments including melanin, hemosiderin, and lipofuscin. MMCs found mainly in different tissues of nonmammalian vertebrates. MMCs are helpful biomarkers for a variety of stresses as environmental pollutants. Aim: This study was designed to elucidate the histological, morphometric, histochemical and immunohistochemical characterization of hepatic MMCs in three reptilian species; Uromastyx ornata (Ornate Dabb lizard), Uromastyx aegyptiaca (Egyptian Dabb lizard), and Varanus griseus (Desert Monitor lizard). Methods: Fifteen adult male reptilian animals were divided into; i. Five adult males Ornate Dabb lizard, ii. Five adult males Egyptian Dabb lizard, iii. Five adult males Desert Monitor lizard. Liver paraffin sections of all animals were processed and stained with Hematoxylin and Eosin stain, Masson Trichrome stain for collagen fibres and Perl's Prussian Blue histochemical stain for hemosiderin pigments. Immunohistochemical demonstration of melanin pigments via HMB-45 and macrophage lineage via CD68 immunostains were applied. Histomorphometric assessments of MMCs were also performed. Results: Histomorphometric examinations revealed significant differences between the histomorphometric structures of MMCs among the experimented species. MMCs in Desert Monitor lizard were fewer in number and smaller in size; with decreased melanin pigment contents and macrophage lineages, and increased hemosiderin contents. Conclusion: The significant differences in MMCs among the three reptiles may indicate considerable differences between the immune system of the Desert Monitor lizard and each of the Ornate and Egyptian Dabb Lizards. turtles), Rhynchocephalia (tuatara) and Squamata (lizards, snakes and worm-lizards). Squamates are the largest reptile order and are subdivided into Lacertilia (lizards and amphisbaenians) and Ophidia (snakes) (Chang et al., 2009;and Rutland et al., 2019). With the exception of some snake species, which are legless, lizards are quadrupedal squamates. Lizards are frequently territorial and have a variety of predator defense mechanisms, including camouflage, venom, and reflex bleeding. Though some reptiles such as land tortoises, some turtles, and some lizards are herbivorous, the majority of reptiles are primarily carnivorous or insectivorous. However, compared to what is required for birds and mammals, the overall amount of food consumed by reptiles is modest (Zaher et al., 2012).Uromastyx aegyptiaca, also known as the Egyptian Dabb lizard or spiny-tailed lizard, is a species of lizard that belongs to the genus Uromastyx, family Agamidae, suborder Iguania, order Squamata, and class Reptilia. It is present across North Africa, particularly in Egypt. Being a purely herbivorous species, Uromastyx aegyptiaca is one of the very few lizard species to have this feeding habit...

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Histological features of the spleen and pancreas of the Egyptian Nile monitor lizard (Varanus niloticus)

Cited by 2 publications

References 27 publications

Does the pancreas of gekkotans differentiate similarly? Developmental structural and 3D studies of the mourning gecko (Lepidodactylus lugubris) and the leopard gecko (Eublepharis macularius)

Does the pancreas of gekkotans differentiate similarly? Developmental structural and 3D studies of the mourning gecko (Lepidodactylus lugubris) and the leopard gecko (Eublepharis macularius)

Melanomacrophage Centers: Comparative Histological Insights among Three Reptilian Species

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