Histological and mechanical evaluation of self‐setting calcium phosphate cements in a sheep vertebral bone void model

Kobayashi, Naomi; Ong, Kevin; Villarraga, Marta L.; Schwardt, J.D.; Wenz, R.; Togawa, Daisuke; Fujishiro, Takaaki; Turner, A. Simon; Seim, Howard B.; Bauer, Thomas W.

doi:10.1002/jbm.a.31079

Cited by 27 publications

(12 citation statements)

References 26 publications

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“…The tissue response to injectable calcium orthophosphate cements is well described in literature [329,362,376,391,392]. Recent histological and mechanical evaluation of self-setting calcium orthophosphate cements in a sheep vertebral bone void model is available elsewhere [393]. The interested readers are also advised to get through a paper on the in vitro biodegradation of brushite cements by a macrophage cell-line [128].…”

Section: Bioresorption and Replacement Of The Self-setting Formulatiomentioning

confidence: 99%

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

Dorozhkin¹

2012

IJMC

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In early 1980s, researchers discovered self-setting calcium orthophosphate cements, which are a bioactive and biodegradable grafting material in the form of a powder and a liquid. Both phases after mixing form a viscous paste that after being implanted sets and hardens within the body as either a non-stoichiometric calcium deficient hydroxyapatite (CDHA) or brushite, sometimes blended with un-reacted particles and other phases. As both CDHA and brushite are remarkably biocompartible and bioresorbable (therefore, in vivo they can be replaced with a newly forming bone), self-setting calcium orthophosphate cements represent a good correction technique of non-weight-bearing bone fractures or defects and appear to be very promising materials for bone grafting applications. Besides, these cements possess an excellent osteoconductivity, molding capabilities, and easy manipulation. Nearly perfect adaptation to the tissue surfaces in bone defects and a gradual bioresorption followed by new bone formation are additional distinctive advantages of calcium orthophosphate cements. Besides, reinforced formulations are available; those are described as calcium orthophosphate concretes. Furthermore, formulations with high viscosity, such as pastes and putties are also known. The discovery of self-setting formulations has opened up a new era in the medical application of calcium orthophosphates; several commercial compositions have already been introduced as a result. Many more formulations are in experimental stages. In this review, an insight into the self-setting calcium orthophosphate formulations, as excellent biomaterials suitable for both dental and bone grafting applications, has been provided.

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Section: Bioresorption and Replacement Of The Self-setting Formulatiomentioning

confidence: 99%

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

Dorozhkin¹

2012

IJMC

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“…The tissue response to injectable calcium orthophosphate cements is well described in literature [265,293,303,318,319]. Recent histological and mechanical evaluation of self-setting calcium orthophosphate cements in a sheep vertebral bone void model is available elsewhere [320]. The interested readers are also advised to get through a recent paper on the in vitro biodegradation of brushite cements by a macrophage cell-line [105].…”

Section: Bioresorption and Replacement Of The Cements By Bonesmentioning

confidence: 99%

Calcium Orthophosphate Cements and Concretes

Dorozhkin¹

2009

Materials

196

145

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In early 1980s, researchers discovered self-setting calcium orthophosphate cements, which are a bioactive and biodegradable grafting material in the form of a powder and a liquid. Both phases form after mixing a viscous paste that after being implanted, sets and hardens within the body as either a non-stoichiometric calcium deficient hydroxyapatite (CDHA) or brushite, sometimes blended with unreacted particles and other phases. As both CDHA and brushite are remarkably biocompartible and bioresorbable (therefore, in vivo they can be replaced with newly forming bone), calcium orthophosphate cements represent a good correction technique for non-weight-bearing bone fractures or defects and appear to be very promising materials for bone grafting applications. Besides, these cements possess an excellent osteoconductivity, molding capabilities and easy manipulation. Furthermore, reinforced cement formulations are available, which in a certain sense might be described as calcium orthophosphate concretes. The concepts established by calcium orthophosphate cement pioneers in the early 1980s were used as a platform to initiate a new generation of bone substitute materials for commercialization. Since then, advances have been made in the composition, performance and manufacturing; several beneficial formulations have already been introduced as a result. Many other compositions are in experimental stages. In this review, an insight into calcium orthophosphate cements and concretes, as excellent biomaterials suitable for both dental and bone grafting application, has been provided.

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“…2,3 Several additives, especially in the form of natural polymers, have been used to correct the physical and biological properties of CPCs. [4][5][6][7] Gelatin is a partially hydrolyzed form of collagen, which is a natural component of bone matrix.…”

mentioning

confidence: 99%

Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements

Orshesh¹,

Hesaraki²,

Khanlarkhani³

2017

IJN

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In recent years, there has been a great interest in using natural polymers in the composition of calcium phosphate bone cements to enhance their physical, mechanical, and biological performance. Gelatin is a partially hydrolyzed form of collagen, a natural component of bone matrix. In this study, the effect of blooming gelatin on the nanohydroxyapatite precipitation, physical and mechanical properties, and cellular responses of a calcium phosphate bone cement (CPC) was investigated. Various concentrations of blooming gelatin (2, 5, and 8 wt.%) were used as the cement liquid and an equimolar mixture of tetracalcium phosphate and dicalcium phosphate was used as solid phase. The CPC without any gelatin additive was also evaluated as a control group. The results showed that gelatin accelerated hydraulic reactions of the cement paste, in which the reactants were immediately converted into nanostructured apatite precipitates after hardening. Gelatin molecules induced 4%–10% macropores (10–300 μm) into the cement structure, decreased initial setting time by ~190%, and improved mechanical strength of the as-set cement. Variation in the above-mentioned properties was influenced by the gelatin concentration and progressed with increasing the gelatin content. The numbers of the G-292 osteoblastic cells on gelatin-containing CPCs were higher than the control group at entire culture times (1–14 days), meanwhile better alkaline phosphatase (ALP) activity was determined using blooming gelatin additive. The observation of cell morphologies on the cement surfaces revealed an appropriate cell attachment with extended cell membranes on the cements. Overall, adding gelatin to the composition of CPC improved the handling characteristics such as setting time and mechanical properties, enhanced nanoapatite precipitation, and augmented the early cell proliferation rate and ALP activity.

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Histological and mechanical evaluation of self‐setting calcium phosphate cements in a sheep vertebral bone void model

Cited by 27 publications

References 26 publications

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

Calcium Orthophosphate Cements and Concretes

Blooming gelatin: an individual additive for enhancing nanoapatite precipitation, physical properties, and osteoblastic responses of nanostructured macroporous calcium phosphate bone cements

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