Histological and Immunohistochemical Observations of  Supramammary Lymph Node in Sheep and Goat

Senthilkumar, S.; Kannan, T.; Ramesh, Geetha; Sumathi, D.

doi:10.20546/ijcmas.2019.808.009

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…The outer layer of the cortex of hamster nodes is represented by a single cortical plateau, and the inner layer is represented by a sphere-shaped unit of the deep cortex. In the guinea pig lymph nodes, the cortex is in the form of a characteristic pronounced mat, the latter appearing as a flattened ring on the sides of which is the capsular trabecula, hence the formation of units of the deep cortex of specific structure, researchers have established the same results (Senthilkumar, et al, 2019).…”

Section: Discussionmentioning

confidence: 80%

“…The relationship between the units of the deep cortex and the afferent lymphatic system has not been verified here because it would have required considerable additional threedimensional reconstruction (Li et al, 2018). However, since all the other morphological characteristics of the units are comparable to those of the rat, it is likely that each unit is also linked to the openings of an afferent lymphatic as in mice, these researches were identified by the authors (Senthilkumar et al, 2019). The absolute size of the units was roughly comparable across the different species, not varying in proportion to the size of the animal nodes.…”

Section: Discussionmentioning

confidence: 91%

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Anatomy of lymph nodes deep cortex in laboratory spices

Fares

Rahmoun

Lieshchova

2019

TAVM

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The lymph nodes are organized lymphoid organs in which lymphocytes are located inside reticular stroma. The lymphoid parenchyma of lymph nodes is divided into separated cell zones, the most developed one is parenchyma in which the deep cortex located. The lymph nodes of small mammals, which are often used for laboratory research, The investigated lymph nodes in this research were Somatic (superficial cervical and axillary) and visceral (hepatic and mediastinal) lymph nodes were selected from mice (n = 5), guinea pigs (n = 5), hamsters (n = 3) and rabbits (n = 5). We used classical histological methods (determination of general histological structure of organs), morphometric (determination of the relative area of individual structural and functional zones), immunohistochemical (determination of location of individual cell populations). It was shown that the deep cortex of the parenchyma of the lymph nodes composed by separate structures «deep cortex units», and some of them are combined into complexes. Each unit is represented by a semicircular structure formed by lymphocytes and reticular fibers. The size of units is approximately the same in all studied animals and does not depend on the size of their bodies. The most developed units of the deep cortex were found in the superficial cervical ganglion, and the largest-in rabbits, where their size reached 3 mm. In all studied animals, units of the deep cortex are located at the same level, along the subcapsular sinus. The most developed component tissue of the studied lymph nodes is the lymphoid parenchyma, in laboratory mice it occupies a significantly larger area than in other animal species. The least developed were the central sections of units in the deep coating crust.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 91%

Anatomy of lymph nodes deep cortex in laboratory spices

Fares

Rahmoun

Lieshchova

2019

TAVM

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“…Bluing the sections was done with running tap water for five minutes. Finally, sections were dehydrated through graded series of alcohol, cleared in xylene and mounted in synthetic mountant (Senthilkumar et al, 2019). Microscopic images of stained slides were captured using the Leica microscope (CH9345 Heer brugg).…”

Section: Immunohistochemistry Staining Protocolmentioning

confidence: 99%

Histological and Immunohistochemical Studies of Furstenberg’s Rosette in Sheep and Goat

Senthilkumar¹

2020

JAR

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A total of twelve healthy adult Madras Red ewe and Boer local she-goat teats were histologically and immunohistochemically examined. In between the teat canal and teat cistern Furstenberg's rosette was appeared as modified zone of mucosa thrown into primary and secondary folds. The mucosal folds were lined by a bilayered cuboidal epithelium. Subepithelial area of rosette was composed of numerous lymphocytes, polymorphonuclear leukocytes, macrophages and plasma cells. Mucosa below the Furstenberg's rosette was lined by stratified squamous keratinized epithelium. Immunohistochemical staining of rosette by CD3 antibody revealed the localization of intraepithelial 'T' lymphocytes in the mucosal folds. 'T' lymphocytes were also localized in the lamina propria region.

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