Histological analysis of effects of 24% EDTA gel for nonsurgical treatment of periodontal tissues

Vasconcellos, Luana Marotta Reis de; Ricardo, Lucilene Hernandes; Balducci, Ivan; Vasconcellos, Luis Gustavo Oliveira de; Carvalho, Yasmin Rodarte

doi:10.2334/josnusd.48.207

Cited by 15 publications

(13 citation statements)

References 21 publications

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“…In this model, ligation acts as a subgingival plaque retentive device that induces mechanical trauma to the dentogingival area, thereby reducing tissue integrity, and allowing plaque formation, thus increasing the number of bacteria and inducing a local destructive inflammatory response in periodontium, which leads to apical migration of the junctional epithelium, loosening of periodontal fibers and alveolar bone resorption (Lohinai et al 1998;Vasconcellos et al 2006). In the induced PD, our morphological results demonstrated numerous PMN inside/or within the epithelial covering and the lamina propria, extending progressively to the alveolar bone crest where numerous osteoclasts were seen from 3 days after induction.…”

Section: Discussionmentioning

confidence: 99%

Morphologic evaluation and expression of matrix metalloproteinases-2 and 9 and nitric oxide during experimental periodontal disease in rat

et al. 2008

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The immunopathologic and inflammatory mechanisms involved in periodontal disease (PD) include the participation of host resident, inflammatory cells and chemical mediators. Metalloproteinases (MMPs) and nitric oxide (NO) play essential role in extracellular matrix turnover of periodontal tissue destruction. In this study, by means of RT-PCR through semi-quantitative densitometric scanning methods, the expression of MMPs -2 and -9 and inducible NO synthase (iNOS) was temporally and spatially investigated during the destructive mechanisms of experimentally induced PD in rats. Samples from different periods were microscopically analyzed and compared with the contralateral side (control). Our results showed significant expression of MMP-9 and iNOS in tissues affected by PD, as compared with controls, three days after PD induction, simultaneously with the beginning of alveolar bone loss. At 7 days post induction, only the MMP-9 mRNA presented a significantly higher expression, as compared with the respective controls. Thus, in the rat ligature-induced PD, MMP-9 and iNOS might importantly participate in the early stages of the disease, including inflammatory cell migration, tissue destruction and alveolar bone resorption. Also, we may suggest that the exuberant presence of PMNs may be related to the important expression of iNOS and MMP-9 found at 3 days post induction.

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Section: Discussionmentioning

confidence: 99%

Morphologic evaluation and expression of matrix metalloproteinases-2 and 9 and nitric oxide during experimental periodontal disease in rat

et al. 2008

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“…[20] In our study, 40 teeth (75.4%) showed repair with cellular cementum (22 teeth, partial and 18 teeth, functional). No anatomical repair was observed in the repaired teeth.…”

Section: Discussionmentioning

confidence: 61%

Cementum and dentin repair following root damage caused by the insertion of self-tapping and self-drilling miniscrews

Ghanbarzadeh

Heravi

Abrishamchi³

et al. 2017

J Orthodont Sci

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OBJECTIVE:The aim of this study was to evaluate the histological responses of cementum, root dentin, and pulp following intentional root injuries caused via self-tapping and self-drilling miniscrews.MATERIALS AND METHODS:Fourteen patients (with a mean age of 15.7 years and age range of 14–18 years) who were scheduled for the extraction of all four first premolars as part of their orthodontic treatment plan participated in this study. The roots of the right and the left quadrants' first premolars were designedly injured using self-tapping miniscrews and self-drilling miniscrews, respectively. Teeth were extracted eight weeks after the injury. Cementum repair was assessed through histological examinations.RESULTS:In this study, 40 teeth (75.4%) showed reparative cementum formation and 13 teeth (24.5%) showed no repair. There was no significant difference between the two groups regarding the formation of reparative cementum (P = 0.3). In all examined teeth, the inflammatory response of the pulp to the cold test was within the normal range.CONCLUSION:This study showed that in most cases, the healing of cementum was observed eight weeks after the injury and the two methods of miniscrew insertion showed no significant difference when it comes to the healing process.

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“…It has been also shown that EDTA gel (24% at pH 7.0) did not affect tissue vitality when used clinically in a nonsurgical periodontal treatment [22], did not interfere with periodontal tissue repair when used histologically during conventional treatment [39], and did not produce any toxicologic problems [25,26,40], thus avoiding the necrotizing effects which may be encountered with other etching agents such as citric and phosphoric acid on the surrounding periodontal tissues.…”

Section: Discussionmentioning

confidence: 98%

Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface

et al. 2012

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Periodontal regeneration using EDTA or PDGF showed promising results, but the effect of combined application was still unclear. This study aimed to verify the effect of EDTA and/or PDGF application on root adhesion and proliferation of PDL fibroblast cells. Eighty specimens were prepared from forty periodontitis teeth and made five groups: (1) diseased (untreated), (2) SRP (scaling root planing), (3) EDTA (24%), (4) PDGF (25 ng/ml) and (5) Combined application of EDTA and PDGF. Periodontal ligament cells were cultured on the above conditioned dentin plate, and SEM examination was preformed and cells were counted within a representative standard area for both cell morphology and density. All groups including untreated showed significantly increase of adhered cells from baseline to 7 days. Among them, rate of increase was much higher in EDTA, PDGF, and combined groups. ANOVA test indicated that the number of cells in PDGF and combined groups was significantly higher than diseased group at 1 day. On day 7, PDGF and combined groups showed significantly higher number of adhesion cells than that found in the diseased, SRP and EDTA groups. Thus, root conditioning with EDTA enhanced cell adhesion more than SRP alone. There was no significant difference of cell number between PDGF and combined group. Combined application of EDTA and PDGF increased significantly PDL cell adhesion than EDTA alone. PDGF alone, however, also showed comparable effect to combined application at all periods. Thus, synergistic effect between PDGF and EDTA was not observed.

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Histological analysis of effects of 24% EDTA gel for nonsurgical treatment of periodontal tissues

Cited by 15 publications

References 21 publications

Morphologic evaluation and expression of matrix metalloproteinases-2 and 9 and nitric oxide during experimental periodontal disease in rat

Morphologic evaluation and expression of matrix metalloproteinases-2 and 9 and nitric oxide during experimental periodontal disease in rat

Cementum and dentin repair following root damage caused by the insertion of self-tapping and self-drilling miniscrews

Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface

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