Histologic Evaluation of Horizontal Frozen Sections

Hematoxylin and Safranin O Staining of Frozen Sections

“…The previous studies by Bentley et al 1 and Miller et al 3 have demonstrated the need to modify histochemical stains to produce a greater accuracy in diagnosis. In 1995, Smith 6 experimented with hematoxylin and safranin O on paraffin sections, and showed contrasting colors of tumor cells in his study of nuclear protein.…”

“…These latter histologic stains are often used to obtain special information that might be helpful. 1 …”

Quantitative and Qualitative Comparison of Two Hematoxylin and Eosin Staining Protocols for Mohs Micrographic Surgery: A Pilot Study

“…Different staining protocols have been developed to maximize tissue processing efficiency while ensuring high-quality staining. 1,2 Quality evaluation of staining protocols commonly relies on subjective analysis.Minimizing tissue staining time may increase efficiency. However, quality of care must not be compromised.…”

“…Different staining protocols have been developed to maximize tissue processing efficiency while ensuring high-quality staining. 1,2 Quality evaluation of staining protocols commonly relies on subjective analysis.…”

Histo‐Clear to Replace Xylene in the Dermatopathology Laboratory

“…We have been using Histo‐Clear for several months as a clearing agent in the same way that we previously used xylene. The photomicrographs of the slides show tissue prepared in the identical manner, except for the use of xylene versus Histo‐Clear 4 . The photomicrographs should speak for themselves; there is no difference between the slides prepared with xylene and the ones prepared with Histo‐Clear (Figs.…”