Histo‐Clear to Replace Xylene in the Dermatopathology Laboratory

Grande, Donald J.; Phillips, Frances W.

doi:10.1111/j.1524-4725.1985.tb02975.x

Cited by 4 publications

(1 citation statement)

References 2 publications

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“…In our opinion, the lack of elimination of xylene in the clearing process may be the cause of the suboptimal results obtained by this method. Replacing it with d- limonene, for example, obtained from the distilled and stabilized light fractions of oils from orange peel and maize kernels, could solve this problem [ 40 ]. Eliminating xylene should not only concern the clearing process, but also the remaining stages of the histopathological protocol, such as cover slipping where xylene-soluble glue is used.…”

Section: Discussionmentioning

confidence: 99%

Modified Histopathological Protocol for Poly-ɛ-Caprolactone Scaffolds Preserving Their Trabecular, Honeycomb-like Structure

et al. 2022

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Poly-ɛ-caprolactone (PCL) is now widely studied in relation to the engineering of bone, cartilage, tendons, and other tissues. Standard histological protocols can destroy the carefully created trabecular and honeycomb-like architecture of PCL scaffolds, and could lead to scaffold fibers swelling, resulting in the displacement or compression of tissues inside the scaffold. The aim of this study was to modify a standard histopathological protocol for PCL scaffold preparation and evaluate it on porous cylindrical PCL scaffolds in a rat model. In 16 inbred Wag rats, 2 PCL scaffolds were implanted subcutaneously to both inguinal areas. Two months after implantation, harvested scaffolds were first subjected to μCT imaging, and then to histopathological analysis with standard (left inguinal area) and modified histopathological protocols (right inguinal area). To standardize the results, soft tissue percentages (STPs) were calculated on scaffold cross-sections obtained from both histopathological protocols and compared with corresponding µCT cross-sections. The modified protocol enabled the assessment of almost 10× more soft tissues on the scaffold cross-section than the standard procedure. Moreover, STP was only 1.5% lower than in the corresponding µCT cross-sections assessed before the histopathological procedure. The presented modification of the histopathological protocol is cheap, reproducible, and allows for a comprehensive evaluation of PCL scaffolds while maintaining their trabecular, honeycomb-like structure on cross-sections.

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Section: Discussionmentioning

confidence: 99%

Modified Histopathological Protocol for Poly-ɛ-Caprolactone Scaffolds Preserving Their Trabecular, Honeycomb-like Structure

et al. 2022

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Xylene Substitutes in Frozen Sections

Whalen

Dufresne

Wilkel

1995

Dermatologic Surgery

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Vegetable oils instead of xylene in tissue processing

Rasmussen

Hjort

Mellerup

et al. 1992

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In an effort to improve working conditions in the histopathologic laboratory, we have investigated whether less toxic substances may be substituted for the toxic organic solvent xylene currently in use. Xylene is used in the clearing as well as the deparaffinization process. We have tested whether the substitution of xylene by olive oil and coconut oil leads to any difference in the quality of the histologic sections. Two tissue blocks from each of 232 specimens sent for histopathologic evaluation were subjected to parallel processing in xylene and oil. The specimens represented a broad spectrum of tissue types. All sections were stained with hematoxylin‐eosin to permit evaluation of histologic and cytologic details. Furthermore, a range of histochemical and immunohistochemical stainings were applied to a subgroup of tissue sections. The results showed qualitative differences between xylene‐processed and oil‐processed tissue in only a minority of cases. In no cases were the oil‐processed samples considered less suitable for histologic diagnosis. In the histochemical and immunohistochemical stainings, no differences were registered. Although the long‐term stability of oil‐processed tissue remains to be clarified, we conclude that less toxic vegetable oil may probably be substituted for xylene without losing valuable diagnostic information.

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Histo‐Clear to Replace Xylene in the Dermatopathology Laboratory

Cited by 4 publications

References 2 publications

Modified Histopathological Protocol for Poly-ɛ-Caprolactone Scaffolds Preserving Their Trabecular, Honeycomb-like Structure

Modified Histopathological Protocol for Poly-ɛ-Caprolactone Scaffolds Preserving Their Trabecular, Honeycomb-like Structure

Xylene Substitutes in Frozen Sections

Vegetable oils instead of xylene in tissue processing

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