Xylene Substitutes in Frozen Sections

Whalen, James; Dufresne, Raymond G.; Wilkel, Caroline S.

doi:10.1111/j.1524-4725.1995.tb00162.x

Cited by 9 publications

(11 citation statements)

References 3 publications

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“…Because of its significant neurotoxicity, and the equal efficacy of readily available substitutes, 11 the use of xylene as a clearing agent has markedly declined. Limonene products are derived from citrus oils and hence have a strong citrus odor, whereas aliphatic xylene substitutes have the advantage of being quicker drying and less oily 10 .…”

Section: Alternative Chemicalsmentioning

confidence: 99%

Assessment and Management of Chemical Exposure in the Mohs Laboratory

Gunson¹,

Smith²,

Vinciullo³

2011

Dermatologic Surgery

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Section: Alternative Chemicalsmentioning

confidence: 99%

Assessment and Management of Chemical Exposure in the Mohs Laboratory

Gunson¹,

Smith²,

Vinciullo³

2011

Dermatologic Surgery

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“…LABORATORY METHODS used to process the frozen sections of Mohs micrographic surgery have evolved by automating the process and by applying immunohistochemical (IHC) techniques 1–5 . While previous surveys obtained information on the cryostat used and its reliability, 6 and about the use of xylene substitutes in processing frozen sections, 7 staining methods currently used were not available. Recently new monoclonal antibodies against proteins specific to melanocytes have provided enhanced detection of melanocytes in frozen sections 8–11 …”

mentioning

confidence: 99%

Current Histologic Preparation Methods for Mohs Micrographic Surgery

Robinson

2001

Dermatol Surg

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Automation of routine slide preparation with a Linistainer decreased staining variability by providing a consistent environment, and decreased processing time. Most laboratories do not perform immunostaining. The relatively high cost of reagents, lack of a reliable automated process, the additional time to process specimens, and the additional technician and physician time makes the procedure impractical for many laboratories.

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“…Xylene substitutes should replace xylene. 31 There are 2 main classes of xylene substitutes: d-limonene and aliphatic hydrocarbons, which are less oily and dry more quickly than the limonene class.…”

Section: Davis and Pellowskimentioning

confidence: 99%

Peripheral Frozen Sections

Davis¹,

Pellowski²

2005

Pathology Case Reviews

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Skin cancer cure rates of 99% can be promised to the patient if the entire peripheral margin of a cutaneous excision is verified to be free of that cancer. Preparing frozen sections to examine only the peripheral margin may seem esoteric to the surgical pathologist. This technique was historically named Mohs' micrographic surgery and is typically performed by a dermatologist in an outpatient clinic, away from the observation of other medical specialists. This treatise provides detailed specifics on how to prepare tissue for peripheral margin evaluation. Along the way, it also provides a brief history of Frederic Mohs' discoveries of the 1930s and how several generations of skin cancer surgeons have refined Mohs' techniques to offer the cutting-edge advantages of the highest cure rates and normal skin sparing.

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Xylene Substitutes in Frozen Sections

Abstract: In light of the improved safety profile and efficacy of the xylene substitutes, they should replace xylene in the Mohs laboratory.

Cited by 9 publications

References 3 publications

Assessment and Management of Chemical Exposure in the Mohs Laboratory

Assessment and Management of Chemical Exposure in the Mohs Laboratory

Current Histologic Preparation Methods for Mohs Micrographic Surgery

Peripheral Frozen Sections

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