Quantitative and Qualitative Comparison of Two Hematoxylin and Eosin Staining Protocols for Mohs Micrographic Surgery: A Pilot Study

Abstract

T he exceptional cure rates achieved with Mohs micrographic surgery (MMS) depend on a surgeon's ability to interpret tissue sections. Accurate interpretation relies on quality staining. Different staining protocols have been developed to maximize tissue processing efficiency while ensuring high-quality staining. 1,2 Quality evaluation of staining protocols commonly relies on subjective analysis.Minimizing tissue staining time may increase efficiency. However, quality of care must not be compromised. This study sought to investigate how an abbreviated hematoxylin and eosin (H&E) staining protocol affects readability of Mohs sections. In addition, this study used image analysis software to analyze MMS tissue staining.