Histogenesis of the nuclei griseum pontis, corporis pontobulbaris and reticularis tegmenti pontis (Bechterew) in the mouse an autoradiographic study

Abstract

By means of autoradiography thc time, site of origin and migration pathways of neuroblasts destined for three nuclei i n the hindbrain of the mouse have been determined. The cells of the nuclei griseum pontis and corporis pontobulbaris arise on gestation days 12 through 16 in the rhombic lip from the obex to the lateral reccss. Most cells migrate within 48 hours via the pontobulbar body to the base of the pons to form the nucleus griseum Fontis; some migrate only a short distance along the pathway and collectively form tho nucleus corporis pontobulbaris. Most cells of thc nucleus reticularis tegnieriti pontis (Bechtcrew) arise in the primitive basal ependymal plate of the pons on gestation days 12 and 13 and migrate ventrally into the tegmentum. Some probably arise at the rhombic lip arid migrate via the pontobulbar body to the base of the ponline flexure where they turn dorsally into the tegmentum.

The Long Journey of Pontine Nuclei Neurons: From Rhombic Lip to Cortico-Ponto-Cerebellar Circuitry

“…This was confirmed by fate mapping with temporally inducible tamoxifen-dependent Atoh1::CreER T2 lines (Machold and Fishell, 2005; Wang et al, 2005) and in utero electroporation in mice (Okada et al, 2007). The Ptf1a + ION neurons are the first ones to be generated and to migrate (E10.5–E11.5), followed by the Atoh1 + LRN (E11.5–12.5), ECN (E11.5–12.5), RTN (E12.5–E13.5) and lastly the BPN (E13.5–E16.5) (Pierce, 1966; Altman and Bayer, 1987d; Machold and Fishell, 2005; Wang et al, 2005; Okada et al, 2007). …”

Rautenlippe Redux—toward a unified view of the precerebellar rhombic lip

“…This proposal was extraordinarily insightful given the limited methodologies and tissue available at that time. Indirect evidence continued to mount in support of a dorsal origin for precerebellar neurons, as revealed by assays including rhombic lip ablations in chick embryos performed in the 1950s [3], as well as isotopic birthdating studies [4] [5-8] and peroxidase labeling studies [9] in rodents performed during the 1960s-1990s. At this point, there seemed little doubt that precerebellar neurons had a dorsal origin; however, the first actual direct evidence was obtained through the use of chick-quail chimeras in the 1990s.…”

Migratory routes and fates of cells transcribing the Wnt‐1 gene in the murine hindbrain

“…In some individuals, the pontine migratory stream disappears as the last of its cells enter the pontine gray nucleus by P0. In others, a few small aggregates of stained cells are found along the former course of the pontine migration and appear to form the bulbopontine nucleus (see Pierce, 1966).…”

“…An extensive literature describes the rhombic lip origins and migrations of the hindbrain neural crest (e.g., Nichols, 1981Nichols, , 1987Tan and Morris-Kay, 1985;Trainor et al, 2002). The rhombic lip and the hindbrain cell populations that migrate from it have been examined using classic morphological stains (see Essick, 1912), experimental manipulation (Harkmark, 1954;Tan and LeDouarin, 1991), autoradiography (Miale and Sidman, 1961;Pierce, 1966Pierce, , 1967Pierce, , 1973Pierce, , 1975Altman and Bayer, 1987a-d), dye lineage tracing (Wingate and Hatten, 1999), viral cell lineage tracing (Lin et al, 2001), and conditional gene expression lineage tracing (Rodriguez and Dymecki, 2000;Fü nfschilling and Reichardt, 2002). In addition, some insights into the genetic regulation of the lip have been gathered Wingate, 2001).…”