Histogenesis of skeletogenic tissue during bone regeneration under distraction

Lavrishcheva, G I; Mikhailova, L. N.

doi:10.1007/bf00798679

Cited by 3 publications

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“…However, it did not become known worldwide until the 1980s, when Italian authors gave it major prominence[ 51 , 52 ]. The approach described by Ilizarov was more than a single apparatus or technique; it became a new paradigm of the cell biology of bone regeneration, and was amply referred to as such in Russian publications during this decade[ 53 ]. By creating a fracture only in the bone cortex (“bone corticotomy”), thus minimising surgical trauma, a callus consolidation process is triggered, and then maintained by means of immobilisation for 7-10 d. Thereafter, continuous slight distraction of less than 1 mm/d is exerted, and over time the gap becomes filled in.…”

Section: Bone Transportationmentioning

confidence: 99%

Challenges of bone tissue engineering in orthopaedic patients

Guerado

Caso

2017

WJO

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Bone defects may impede normal biomechanics and the structural stability of bone as an organ. In many cases, the correction of bone defects requires extensive surgical intervention involving the use of bone-grafting techniques and other procedures in which healing is slow, there is a high risk of infection and considerable pain is provoked - with no guarantee of complete correction of the defect. Therefore, the search for surgical alternatives continues to present a major challenge in orthopaedic traumatology. The reamer-irrigator-aspirator (RIA) system, which was devised to avoid the problems that can arise with autograft harvesting from the iliac crest, consists of collecting the product of the femoral canal after reaming. The RIA technique improves osteogenic differentiation of mesenchymal stem cells, compared to bone marrow aspiration or cancellous bone harvesting from the iliac crest using a spoon. Another approach, the Masquelet technique, consists of reconstructing a long bone defect by means of an induced membrane grown onto an acrylic cement rod inserted to fill the defect; in a second surgical step, once the membrane is constituted, the cement rod is removed and cancellous autograft is used to fill the defect. Both in RIA and in the Masquelet technique, osteosynthesis is usually needed. Bone transportation by compression-distraction lengthening principles is commonly implemented for the treatment of large bone loss. However, complications are frequently encountered with these techniques. Among new techniques that have been proposed to address the problem of large bone loss, the application of stem cells in conjunction with tissue engineering techniques is very promising, as is the creation of personalised medicine (or precision medicine), in which molecular profiling technologies are used to tailor the therapeutic strategy, to ensure the right method is applied for the right person at the right time, after determining the predisposition to disease among the general population. All of the above techniques for addressing bone defects are discussed in this paper.

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Section: Bone Transportationmentioning

confidence: 99%

Challenges of bone tissue engineering in orthopaedic patients

Guerado

Caso

2017

WJO

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Morphological Aspects of Bone Regeneration in Cortical Bone Lengthening Using Ilizarov’s Technique

Delloye¹,

Delefortrie²,

Coutelier³

1990

Bone Circulation and Bone Necrosis

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Osteogenesis and angiogenesis during distraction osteosynthesis

Шевцов¹,

Ir’yanov²

1995

Bull Exp Biol Med

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The volume-spatial organization of osseous tissue and the microarchitectonics of distraction bone regenerating regions of 80 dogs with crus elongation performed after Ilizarov are studied at different stages using the methods of light microscopy, transmission and scanning electron microscopy, and x-ray electron probe microanalysis. It is shown that the rarefaction the interstitial spaces resulting from controlled distension of the bone fragments exerts a stimulatory and orienting effect on angiogenesis. The vascular loops from the proximal and distal fragments and from the paraosseous tissue grow in opposite directions, meet, and intrude into the opposite fragments. Primary osteons develop around these loops. The rapid elongation of osteons insure the growth of the regenerating bone tissue in the desired longitudinal direction. Key Words: osteogenesis; angiogenesis; limb elongationThe high efficacy of the modalities of treatment established under the supervision of G. A. Ilizarov is well known. At the same time, the data concerning morphological peculiarities of the osseous tissue and blood vessels in the regions of distraction bone regeneration are scant and controversial [1][2][3][4].The goal of this work was to study the volume-spatial specific features of the bone tissue and the vessels of the microcirculatory bed in bone regenerates formed during limb elongation after the method of Ilizarov. MATERIALS AND METHODSEighty adult dogs underwent closed flexion osteoclasia of the tibiae. Five days later a distraction (controlled distension of the bone fragments) was started using the device developed by llizarov, at a daily rate of 0.75-1 ram, in 4 procedures per day. 14, 21, 28, and 42 days after the start of distraction, 1 and 2 months after subsequent fixation of the limb in the apparatus, and 3 and 6 months after removal of the apparatus. The tibial epimetaphyseal regions of 18 1-6-month-old puppies were also studied. Microarchitectonic and morphofunctional peculiarities of the osseous tissue and blood vessels in the regions of bone regeneration were studied on cleared unstained preparations and on semithin sections stained with Schiff reagent and methylene blue. The study also included transmission electron microscopy of ultrathin sections and scanning electron microscopy of polished bone sections, corrosion preparations, and injectional corrosion replicas of blood vessels [5]. The distribution and concentration of calcium in the bone sections were recorded using an x-ray electron probe microanalyzer. RESULTSAnalysis of the bone marrow channels in the fragments adjacent to the fracture line five days postoperation reveals the formation of a fine-mesh

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Histogenesis of skeletogenic tissue during bone regeneration under distraction

Cited by 3 publications

References 0 publications

Challenges of bone tissue engineering in orthopaedic patients

Challenges of bone tissue engineering in orthopaedic patients

Morphological Aspects of Bone Regeneration in Cortical Bone Lengthening Using Ilizarov’s Technique

Osteogenesis and angiogenesis during distraction osteosynthesis

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