The volume-spatial organization of osseous tissue and the microarchitectonics of distraction bone regenerating regions of 80 dogs with crus elongation performed after Ilizarov are studied at different stages using the methods of light microscopy, transmission and scanning electron microscopy, and x-ray electron probe microanalysis. It is shown that the rarefaction the interstitial spaces resulting from controlled distension of the bone fragments exerts a stimulatory and orienting effect on angiogenesis. The vascular loops from the proximal and distal fragments and from the paraosseous tissue grow in opposite directions, meet, and intrude into the opposite fragments. Primary osteons develop around these loops. The rapid elongation of osteons insure the growth of the regenerating bone tissue in the desired longitudinal direction. Key Words: osteogenesis; angiogenesis; limb elongationThe high efficacy of the modalities of treatment established under the supervision of G. A. Ilizarov is well known. At the same time, the data concerning morphological peculiarities of the osseous tissue and blood vessels in the regions of distraction bone regeneration are scant and controversial [1][2][3][4].The goal of this work was to study the volume-spatial specific features of the bone tissue and the vessels of the microcirculatory bed in bone regenerates formed during limb elongation after the method of Ilizarov. MATERIALS AND METHODSEighty adult dogs underwent closed flexion osteoclasia of the tibiae. Five days later a distraction (controlled distension of the bone fragments) was started using the device developed by llizarov, at a daily rate of 0.75-1 ram, in 4 procedures per day. 14, 21, 28, and 42 days after the start of distraction, 1 and 2 months after subsequent fixation of the limb in the apparatus, and 3 and 6 months after removal of the apparatus. The tibial epimetaphyseal regions of 18 1-6-month-old puppies were also studied. Microarchitectonic and morphofunctional peculiarities of the osseous tissue and blood vessels in the regions of bone regeneration were studied on cleared unstained preparations and on semithin sections stained with Schiff reagent and methylene blue. The study also included transmission electron microscopy of ultrathin sections and scanning electron microscopy of polished bone sections, corrosion preparations, and injectional corrosion replicas of blood vessels [5]. The distribution and concentration of calcium in the bone sections were recorded using an x-ray electron probe microanalyzer. RESULTSAnalysis of the bone marrow channels in the fragments adjacent to the fracture line five days postoperation reveals the formation of a fine-mesh
The stimulatory effect of a local focus of granulation tissue, created in the bone marrow cavity, on reparative osteogenesis after tibial bone fracture was detected in experimental rats by microscopic examination of histological sections, scanning electron microscopy, and X-ray electron probe microanalysis.
Aim: To study morphological features of the bone formation process in consolidation of fractures of long tubular bones in conditions of (Вестник РАМН. 2014; 7-8: 127-132) ВведениеПроблема совершенствования медицинской помощи больным с повреждениями опорно-двигательной систе-мы с каждым годом приобретает все большее значение в связи с увеличением числа пострадавших, инвалидности и смертности от травм, не имеющими тенденции к сни-жению. Однако реальные сроки остеосинтеза продол-жают оставаться значительными. С целью оптимизации условий формирования регенерата кости, сокращения сроков лечения и профилактики осложнений используют метод направленной стимуляции процесса регенерации костной ткани путем применения интрамедуллярных спиц с кальций-фосфатным покрытием [1][2][3][4]. Исследо-вание, проведенное нами ранее, свидетельствует о более активных процессах костеобразования, минерализации и органотипической перестройки в регенератах костей, формирующихся при удлинении конечности в условиях интрамедуллярного введения спиц с кальций-фосфат-ным покрытием [5]. Вместе с тем процесс репаративного остеогенеза с использованием тех или иных имплантатов, введенных в регенерат кости, еще недостаточно изучен; также отсутствуют характеристики эффективности и ме-ханизма их действия.Цель исследования: изучить морфологические осо-бенности костеобразовательного процесса при консоли-
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