Histochemistry of the duodenal glands of the cat and horse

Abstract

The duodenal glands of cat and horse were studied using PAS, Alcian blue, dialysed iron, aldehyde fuchsin-Alcian blue and high iron diamine stains. It was found that the duodenal glands of the horse reacted positively to Alcian blue, dialysed iron stains and also took the Alcian blue stain in the combined aldehyde fuchsin-Alcian blue and high iron diamine-Alcian blue stains. Those of the cat gave negative results. These results suggest the presence of acidic groups in the mucosubstances secreted by the horse’s duodenal glands. A suggestion is put forward on the strength of the high iron diamine-Alcian blue combined stains that the acidity is due to the presence of carboxyl groups. It is suggested that the acidity may be significant in either the cellulose metabolism or the digestion of the bacterial microflora from the stomach of herbivores.

Immunocytochemical distribution of endocrine cells in the gastrointestinal tract of the horse

“…Somatostatin-, gastrin-and cholecystokinin-immunoreactive cells are present in Brunner's glands of the horse. Endocrine cells in the duodenal glands are presumed to influence the secretion by EQUINE VETERINARY JOURNAL Brunner's glands which also may be influenced by dietary habit (Oduor-Okelo 1976), as well as peptides released from surrounding nerve fibres (Kirkegaard ef a1 1981).…”

Comparative glycopattern analysis of mucins in the Brunner's glands of the guinea-pig and the house mouse (Rodentia)

“…and the golden hamster, and some marsupials, such as the koala and the common wombat (Krause, 2000;Schumacher et al, 2004). The functions of the neutral mucins are probably different from those of the acidic ones: as an example, Oduor-Okelo (1976) hypothesizes that in herbivores the acidity could be important in either cellulose metabolism or the digestion of the bacterial microflora. Anyway, both Forman (1971) on bats and Schumacher et al (2004) on a number of primates, carnivores, rodents, lagomorphs and artiodactyls do not find any relation between the presence of acidic mucins and dietary adaptations.…”

Duodenal Glands of the Pony (Equus caballus)

“…The secretion of the duodenal gland is presumed to play an important role in the physiology of digestion. There are only a few papers published to report histochemical srudies on the composition of the secretion of this gland (COCHRANE et al, 1964;MIRAGLIA et al, 1967;ODUOR-OKELO, 1976;SHEAHAN/JERVIS, 1976;CAROLYN/PHILIP, 1976;PODDAR/JACOB, 1979;SUZUKI et al, 1981).…”

“…England) (SPICER et al, 1967), AB(pH2.5)-PAS (PEARSE, 1985), Lipase reaction (GOMORI, 1946 a, b) and peroxidase(P0)-labeled Lectin (LT) [Concanavalin A (Con A), peanut agglutinin (PNA), R i c k s communis agglutinin-I (RCA-I), Ulex europaeus agglutinin-I (UEA-I), Dolichos biflorus agglutinin (DBA), wheat germ agglutinin (WGA), Limulus polyphemus agglutinin (LPA), soybean agglutinin (SBA), Maclura pomifera agglutinin (MPA), G~iffo~ia simpli&folia-I (GS-I), G~ifforiu simplicifoliu-I1 (GS-1I)J-diaminobenzidine (DAB). Endogenous peroxidase in the tissue sections was blocked by incubation with 2 % H202 for 10 min.…”

“…The enzyme, horseradish peroxidase, was visualized by incubation for 8-10 min in PBS solution containing diaminobenzidine (DBA) and H202. The sections were then washed in tap water for 5 min, dehydrated, and coverslipped with Permount (PEARSE, 1985). Control procedures for Po-LT-DAB staining were as follows: we employed, as the particular monosaccharide, 0.1 M of a-methyl-D mannoside for Con A, lactose for PNA, D-galactose for RCA-I, MPA and GS-I, L-fucose for UEA-I, N-acetyl-D-galactosamine for DBA and SBA, N-acetyl-Dglucosamine for WGA and GS-I1 and N-acetyle neuraminic acid for LPA.…”