Comparative glycopattern analysis of mucins in the Brunner's glands of the guinea-pig and the house mouse (Rodentia)

Scillitani, Giovanni; Mentino, Donatella

doi:10.1016/j.acthis.2015.06.003

Cited by 16 publications

(13 citation statements)

References 51 publications

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“…Thus, our results might indicate that in the ileum WGA binding is possibly inhibited by the high sulfation of terminal sialic acid residues. These results are consistent with other studies in rodents which have shown a higher labeling intensity of certain lectins, including WGA, after a pretreatment desulfation (Liquori et al, ; Mastrodonato et al, ; Scillitani and Mentino, ). Lectin‐histochemical methods combined with chemical and enzymatic treatments could be used in future studies to confirm the presence of highly sulfated sialic acid residues in the ileum of L. maximus .…”

Section: Discussionsupporting

confidence: 93%

Comparative Analysis of the Morphology, Ultrastructure, and Glycosylation Pattern of the Jejunum and Ileum of the Wild Rodent Lagostomus maximus

Hoz

Flamini

Díaz

2016

The Anatomical Record

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Morphological and histochemical analyses were performed to characterize the histology, ultrastructure, and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus. Enterocytes, goblet cells, Paneth cells, and enteroendocrine cells were identified in both intestinal epithelia. Two morphological types of enterocytes were identified only in the ileum based on their cytoplasm electron density. Although the histological and ultrastructural examination showed that the epithelia of both anatomical regions were morphologically similar, a certain specialization in their secretory products was evident. The glycosylation pattern of the jejunum and ileum was characterized in situ by histochemical and lectin histochemical methods. Histochemical results revealed the presence of carboxylated and sulfated gycoconjugates in both regions, although sulfomucins were clearly prevalent in the ileum. Sialic acid was highly O-acetylated and particularly abundant in the jejunum. The KOH/PA*/Bh/PAS technique evidenced a more intense histochemical reaction in the jejunal than in the ileum goblet cells, demonstrating a reduction of neutral mucin secretion in the distal small intestine. Further specific differences were revealed by lectin histochemistry. These data evidenced that the nature of mucus varies at different anatomical regions, probably adapted to physiological requirements. Anat Rec, 299:630-642, 2016. V C 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 93%

Comparative Analysis of the Morphology, Ultrastructure, and Glycosylation Pattern of the Jejunum and Ileum of the Wild Rodent Lagostomus maximus

Hoz

Flamini

Díaz

2016

The Anatomical Record

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“…However, the Brunner's glands of the mouse produce class-III stable neutral mucins that are binding to the same lectins as the ones observed in the guinea-pig, not including those specific to sialic acid. In contrast to Brunner's glands, in the mouse, the duodenal goblet cells do not have stable class-III mucins and present tiny amounts of sialic acid [7,9].…”

Section: Discussionmentioning

confidence: 87%

“…Some comparative data regarding the mucins secreted by the Brunner's glands and the duodenal goblet cells in the chinchilla, guinea-pig and the house mouse were provided by some authors [7,8].…”

Section: Discussionmentioning

confidence: 99%

Comparative features of the mucous glands in the gastroduodenal junction in guinea pigs and chinchillas: microanatomy and histochemistry

Gal¹,

Rus²,

Andrei³

et al. 2019

Preprint

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Background: Mucous glands from the gastroduodenal junction are briefly represented by glandular cells in the stomach and by Brunner’s glands in the duodenum. The aim of the study was to describe the main histological/histochemical features of the gastroduodenal junction in guinea pigs and chinchillas. Results: The material was represented by tissue samples collected from the stomach and duodenum processed by paraffin technique. Histological sections were stained by Goldner’s trichrome stain, while mucous content was identified by Periodic acid–Schiff (PAS) stain (for neutral mucosubstances) and Alcian blue stain (for acid mucosubstances). In guinea pig, the pyloric glands were positive for both mucus types, whereas in chinchillas a low amount of mucin was identified. The Bruner glands, in guinea pigs displayed a weak reaction for the both mucin types. In chinchillas, the Bruner glands showed a significantly higher amount of PAS and Alcian blue-positives mucosubstances types. As observed, a higher amount of neutral mucus was identified in the pyloric glands in guinea pigs comparing to chinchillas. Contrariwise, chinchillas displayed a suggestively higher amount of mucus in Bruner glands comparing to guinea pigs. Conclusions: Concluding, this is the first report describing the comparative features of the mucous glands in the two rodents, with detailed histological and histochemical features.

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“…After 1 h incubation at room temperature in the appropriate lectin solution in 4‐(2‐hydroxyethyl)‐1‐piperazine‐ethane‐sulphonic acid (HEPES), sections were rinsed in the same buffer and mounted in Fluoromount (Sigma‐Aldrich) for observation. Three different methods were performed as controls for lectin labelling: (a) incubation with lectin‐free HEPES, (2) incubation with lectin plus an inhibitory sugar (types and concentrations given in Table ), and (c) binding to secreting epithelia of the duodenum of the mouse or guinea‐pig, as well as to the egg extracellular matrix of the toad Bufo bufo , the mucins of which have been previously demonstrated to be labelled by the tested lectins (Mentino et al ., ; Scillitani & Mentino, ).…”

Section: Methodsmentioning

confidence: 99%

Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes)

Carlucci

Mentino

Semeraro

et al. 2019

Journal of Fish Biology

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The localization of intestinal glycoconjugates of the blunthead pufferfish Sphoeroides pachygaster and the grey triggerfish Balistes capriscus from the north-western Ionian Sea was analysed by histochemical methods (PAS, AB pH 2.5, HID) and lectin binding experiments (WGA, LFA, SBA, sialidase-SBA, PNA, sialidase-PNA, ConA, AAA, UEA-I, LTA) to assess how evolutionary loss of a functional stomach in S. pachygaster affects intestinal secretions relative to the B. capriscus, which retains the plesiomorphic gastric condition. Sphoeroides pachygaster had a lower content of acid mucins but more complex sialylation patterns than B. capriscus. GalNAc and GlcNAc residuals were present in both, but GalNAc residuals in S. pachygaster were subterminal to sialic acid. Balistes capriscus lacked galactosylated residuals and its enterocytes had a glycocalyx that differed in composition between the small intestine and the rectum and was missing from S. pachygaster. Functional and ecological implications of these findings are discussed.

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Comparative glycopattern analysis of mucins in the Brunner's glands of the guinea-pig and the house mouse (Rodentia)

Cited by 16 publications

References 51 publications

Comparative Analysis of the Morphology, Ultrastructure, and Glycosylation Pattern of the Jejunum and Ileum of the Wild Rodent Lagostomus maximus

Comparative Analysis of the Morphology, Ultrastructure, and Glycosylation Pattern of the Jejunum and Ileum of the Wild Rodent Lagostomus maximus

Comparative features of the mucous glands in the gastroduodenal junction in guinea pigs and chinchillas: microanatomy and histochemistry

Comparative histochemical analysis of intestinal glycoconjugates in the blunthead pufferfish Sphoeroides pachygaster and grey triggerfish Balistes capriscus (Teleostei: Tetraodontiformes)

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