Comparative Analysis of the Morphology, Ultrastructure, and Glycosylation Pattern of the Jejunum and Ileum of the Wild Rodent Lagostomus maximus

Abstract

Morphological and histochemical analyses were performed to characterize the histology, ultrastructure, and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus. Enterocytes, goblet cells, Paneth cells, and enteroendocrine cells were identified in both intestinal epithelia. Two morphological types of enterocytes were identified only in the ileum based on their cytoplasm electron density. Although the histological and ultrastructural examination showed that the epithelia of both anatomical regions were morphologically similar, a certain specialization in their secretory products was evident. The glycosylation pattern of the jejunum and ileum was characterized in situ by histochemical and lectin histochemical methods. Histochemical results revealed the presence of carboxylated and sulfated gycoconjugates in both regions, although sulfomucins were clearly prevalent in the ileum. Sialic acid was highly O-acetylated and particularly abundant in the jejunum. The KOH/PA*/Bh/PAS technique evidenced a more intense histochemical reaction in the jejunal than in the ileum goblet cells, demonstrating a reduction of neutral mucin secretion in the distal small intestine. Further specific differences were revealed by lectin histochemistry. These data evidenced that the nature of mucus varies at different anatomical regions, probably adapted to physiological requirements. Anat Rec, 299:630-642, 2016. V C 2016 Wiley Periodicals, Inc.

Histochemistry of the zona pellucida of the ovary of a species with natural polyovulation: Lagostomus maximus (Rodentia, Hystricomorpha, Chinchillidae)

“…The α‐amylase‐PAS, K(OH)/PA*/Bh/PAS and PA/Bh/KOH/PAS techniques were used to study the presence of sugars, usually in organs that produce different types of mucins, since they allow differentiating certain features of a specific portion of carbohydrates (Lev & Spicer, ; McManus, ; Pearse, ; Reid, Culling, & Dunn, ; Volz, Reid, Park, Owen, & Dunn, ). In L. maximus, these techniques were applied to organs of the digestive system (Tano de la Hoz et al, ; Tano de la Hoz, Flamini, Zanuzzi, & Díaz, ). So far, there have been no studies referred to the ZP of the ovary of mammals using these techniques.…”

“…Of each ovary, no less than 15 follicles in which the ZP was evident were observed; among them: preantral, secondary, tertiary, polyovulatory and atretic follicles. The intensities of the different techniques were classified according to the following semi‐quantitative scale: 0, unlabelled; 1, light mark; 2, moderate mark; and 3, strong mark, according to the criterion used in previous works (Barbeito, Ortega, Matiller, Gimeno, & Salvetti, ; Boonzaier, Merwe, Bennett, & Kotze, ; Liquori et al, ; Mastrodonato, Mentino, Liquori, & Ferri, ; Plaul, Barbeito, & Díaz, ), including some carried out in the species under study (Flamini et al, ; Tano de la Hoz, Flamini, & Díaz, ).…”

Development of the digestive system of Argentine hake, Merluccius hubbsi, larvae

“…The results were evaluated by independent observers using a semi‐quantitative scale to determine the intensity of the reactions (0, negative reaction; 1, weak positive reaction; 2, moderate positive reaction, and 3, strong positive reaction). This classification was established according to previous histochemical studies (Carlucci et al, 2019; Cohen et al, 2014; Scillitani & Mentino, 2015; Tano de la Hoz, Flamini, & Díaz, 2014, 2016).…”

The colonic groove of the plains viscacha (Lagostomus maximus): Histochemical evidence of an abrupt change in the glycosylation pattern of goblet cells

“…The results were evaluated by four independent observers using a semi‐quantitative scale to determinate the intensity of the reactions (0, negative; 1, light; 2, moderate; 3, strong). These scores were established according to previous histochemical studies (Scillitani & Mentino, ; Tano de la Hoz et al, ).…”

“…The physicochemical characteristics of mucins are determined mainly by their composition and their content of glycans (Liquori et al, ). Consequently, the analysis of the glycosylation pattern of mucins has been widely used in diverse studies to infer its physiological role (Mastrodonato, Mentino, Liquori, & Ferri, ; Scillitani & Mentino, ; Tano de la Hoz, Flamini, & Díaz, ).…”