The colonic groove of the plains viscacha (Lagostomus maximus): Histochemical evidence of an abrupt change in the glycosylation pattern of goblet cells

Abstract

The ascending colon of most rodent species shows a longitudinal colonic groove that works as a retrograde transport pathway for a mixture of bacteria and mucus toward the cecum. We describe the morphology and glycosylation pattern of the colonic groove of Lagostomus maximus to analyze the role of mucins in this anatomical feature. We also studied the distribution pattern of the interstitial cells of Cajal (ICC) to evaluate their regulatory influence on gut motility. The groove originated near the cecocolic junction and extended along the mesenteric side of the ascending colon, limited at both ends by nonpapillated ridges. These ridges divided the lumen of the ascending colon into two compartments: a narrow channel and a large channel, called the groove lumen and the main lumen, respectively. The histochemical analysis showed differences in the glycosylation pattern of the goblet cells inside and outside the groove. Unlike the mucosa lining the main lumen of the colon, the groove was rich in goblet cells that secrete sulfomucins. The PA/Bh/KOH/PAS technique evidenced an abrupt change in the histochemical profile of goblet cells, which presented a negative reaction in the groove and a strongly positive one in the rest of the colonic mucosa. The anti-c-kit immunohistochemical analysis showed different ICC subpopulations in the ascending colon of L. maximus. Of all types identified, the ICC-SM were the only cells located solely within the colonic groove.

Histochemistry of the zona pellucida of the ovary of a species with natural polyovulation: Lagostomus maximus (Rodentia, Hystricomorpha, Chinchillidae)

“…The α‐amylase‐PAS, K(OH)/PA*/Bh/PAS and PA/Bh/KOH/PAS techniques were used to study the presence of sugars, usually in organs that produce different types of mucins, since they allow differentiating certain features of a specific portion of carbohydrates (Lev & Spicer, ; McManus, ; Pearse, ; Reid, Culling, & Dunn, ; Volz, Reid, Park, Owen, & Dunn, ). In L. maximus, these techniques were applied to organs of the digestive system (Tano de la Hoz et al, ; Tano de la Hoz, Flamini, Zanuzzi, & Díaz, ). So far, there have been no studies referred to the ZP of the ovary of mammals using these techniques.…”

The stomach of the wild herbivorous hystricomorph rodent Lagostomus maximus: A comparative histomorphological and histochemical study of the cardiac and pyloric regions

“…Diverse studies have demonstrated that the rodent L. maximus is exclusively herbivorous and feeds on a great variety of plant species, such as annual and perennial herbs and small shrubs (Giuletti & Jackson, 1986; Jofre, 1994), showing preference for grasses (Bontti, Boo, Lindstrom, & Elia, 1999) and dicotyledons (Kufner & Monge, 1998). As part of its digestive strategy, it practices coprophagia, ingesting at least certain fraction of its own faeces, rich in proteins, minerals and vitamins, although poor in fibres (Clauss et al., 2007; Tano de la Hoz et al., 2017). This strategy is important to its absorptive activity, as it happens in other hystricomorphs (de Barros Moraes et al., 2002).…”

“…The results were evaluated by four independent observers using a semi‐quantitative scale to determinate the intensity of the reactions (0, negative; 1, weak; 2, moderate; 3, strong). These scores were established according to previous histochemical studies (Tano de la Hoz et al., 2014, 2017; Tano de la Hoz, Flamini, Portiansky, & Díaz, 2019).…”

“…In the last decade, diverse research has been done on the reproductive biology (Acuña et al., 2019; Flamini, Barbeito, & Portiansky, 2014; Flamini et al., 2011; Halperin, Dorfman, Fraunhoffer, & Vitullo, 2013) and endocrine glands (Rodriguez et al., 2013) of L. maximus. Although there are some contributions on the digestive system of this rodent, few are the studies that relate its morphology to physiology (Clauss et al., 2007; Hagen et al., 2015; Tano de la Hoz et al., 2014; Tano de la Hoz, Flamini, Zanuzzi, & Díaz, 2017).…”

Glycan expression as a tool for a deeper understanding of a reproductive gland in a skate of economic importance

“…The intensities of the different lectins were classified according to the following semi‐quantitative scale: 0, unlabelled; 1, light stain; 2, moderate stain; and 3, intense stain, according to the criterion used in previous studies (Acuña et al ., 2018; Tano de la Hoz et al ., 2016, 2017). The evaluation of the intensity of each reaction was based on estimates from three trained independent researches, blinded to the samples being observed.…”