Histochemistry and Cytochemistry of Glycoproteins and Glycosaminoglycans

Wood, John G.; McLaughlin, Barbara J.

doi:10.1007/978-1-4613-2925-1_7

Cited by 8 publications

(2 citation statements)

References 118 publications

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“…They are capable of binding different sugars with relative specificity, and provide important information about the presence of specific glycoconjugates and their discrete localization. However, in some cases it is difficult to identify individual macromolecules, because many glycoproteins and some glycolipids contain the common sugars that serve as lectin receptors (Wood and McLaughlin, 1979;Gabius, 2001). …”

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Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Rueda¹,

Cantos²,

Lim³

2003

Anat. Rec.

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During development, different epithelial cells in the mouse cochlea express different cell surface glycoconjugates, which may reflect membrane specialization. Some of the lectins tested in this study (SBA, succ-WGA, and PSA) labeled the sensory cells of the cochlea around birth. Other lectins (WGA, Con A, RCA-II, and PHA-E) labeled surfaces of the sensory cells, particularly the stereocilia, from early stages of development (gestation day (GD) 16) through 21 days after birth. These may be adhesion molecules needed to attach the newly forming tectorial membrane (TM) to the stereocilia. Lectin staining of the developing TM revealed that the substructures of the TM are biochemically distinct. Lectin staining also showed the temporal sequence of the expression of cytoplasmic glycoconjugates of the cochlear epithelium during development. Biochemical changes during development are probably the result of different cells being involved in the production of glycoconjugates, and may have functional significance, specifically with regard to the expression of adhesion and/or signaling molecules. Anat Rec Part A 274A: 923-933, 2003.

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confidence: 99%

Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Rueda¹,

Cantos²,

Lim³

2003

Anat. Rec.

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“…However, the location of most of the individual species of glycocon jugates in the brain remains unknown, and understanding of their biologic significance is accordingly limited. Immunostaining has localized glycoproteins in nervous tissue [2] and has shown, for example, a glycoprotein obtained from purified myelin [3] in Oligo dendroglia and myelin sheaths [4], Labelled lectins possessing affinity for specific termi nal sugars or internal sugar linkages in giycoconjugates [5] have afforded a means of localizing and partially characterizing car bohydrate-rich components in the brain [2], Recent histochemical studies with label led lectins have localized glycoconjugate con taining terminal /V-acetylgalactosamine (Gal/VAc) at the surface of a subpopulation of cortical neurons and certain other neurons in brain [6]. Evidence has been obtained that some of the neurons with surface staining for Gal/VAc are GABAergic but some are not [7].…”

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Postnatal Appearance of Glycoconjugate with Terminal N-Acetylgalactosamine on the Surface of Selected Neurons in Mouse Brain

Nakagawa

Schulte

et al. 1987

Dev Neurosci

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Selected neurons in the cortex and other regions of the brain of adult mice stain with Vicia villosa agglutinin (WA) conjugated to horseradish peroxidase (HRP). This staining demonstrates glycoconjugate with terminal N-acetylgalactosamine (GalNAc) in periodic foci on the neuronal surface. However, brains from mice at 1 week of age lacked neurons with surface reactivity for VVA-HRP but showed neurons with faint cytoplasmic staining. Two weeks postnatally, infrequent cortical neurons located mostly in layer IV disclosed light surface affinity for VVA-HRP. The deep cerebellar nucleus at 2 weeks revealed WA-positive neurons that were less abundant than in adults but exceeded in number the stained neurons in the cortex of 2-week-old mice. By 3 weeks of age the prevalence of positive neurons in cortical layers II-VI and the deep cerebellar nucleus and the intensity of the surface staining approximated that observed in adult mice. No staining of fiber tracts was observed 1 week postnatally. However, certain tracts exemplified by decussating fibers in the basal pontine region showed strong VVA-HRP reactivity in brains of 2-week-old mice as in adults. Acquisition of WA surface affinity 3 weeks postnatally coincided with the appearance of cytosolic immunostaining for glutamic acid decarboxylase in a population of GABAergic neurons in the cortex. These findings demonstrate the postnatal development of GalNAc-containing glycoconjugate associated with selected neurons and the variable acquisition of the glycoconjugate among these neurons possibly related to onset of function.

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Selective cytochemical demonstration of glycoconjugate‐containing terminal N‐acetylgalactosamine on some brain neurons

Nakagawa¹,

Schulte²,

Spicer³

1986

J of Comparative Neurology

133

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Paraffin embedded sections of rat, mouse, dog, and human brain were stained with a battery of lectin-horseradish perioxidase conjugates to localize and characterize glycoconjugates. In the rat and mouse cerebral cortex, a subpopulation of nonpyramidal neurons stained selectively with three lectins with specific affinity for terminal N-acetylgalactosamine (GalNAc). These and only these lectins stained the surface of the cell body, dendritic shafts, and proximal parts of the dendritic arborization. Most reactive, nonpyramidal neurons revealed a multipolar dendritic pattern, but some possibly belonged to the bitufted and bipolar types of neuron. The GalNAc-containing neurons appeared widely distributed in layers II-VI with relatively greater abundance in layers IV and V. In the cortex of rats and mice the stained neurons occurred in moderate numbers in the frontal, frontoparietal, striate, retrosplenial, and entorhinal regions, but were less numerous in the hippocampal gyrus, dentate gyrus, and olfactory area. Other neurons in the basal ganglia and brain stem stained weakly for GalNAc. Examination of the frontal cortex of human and canine brains showed a similar distribution of nonpyramidal neurons with affinity for GalNAc-binding lectins. At high magnification, the surface staining of neurons in the cerebral cortex, deep cerebellar nucleus, and other sites appeared periodic rather than continuous. The periodic character of the neuronal surface staining suggested a location for the reactive glycoconjugate in or between the synapses. The GalNAc-containing glycoconjugate occurred in a selected cell type, failed to bind the other lectin conjugates, and differed from biochemically detected glycoconjugates. It is, therefore, considered a newly recognized entity of possible physiologic significance for a population of cortical neurons.

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Histochemistry and Cytochemistry of Glycoproteins and Glycosaminoglycans

Cited by 8 publications

References 118 publications

Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Postnatal Appearance of Glycoconjugate with Terminal N-Acetylgalactosamine on the Surface of Selected Neurons in Mouse Brain

Selective cytochemical demonstration of glycoconjugate‐containing terminal N‐acetylgalactosamine on some brain neurons

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