Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Rueda, Joaquı́n; Cantos, Raquel; Lim, David J.

doi:10.1002/ar.a.10109

Cited by 10 publications

(12 citation statements)

References 58 publications

(67 reference statements)

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“…2a,b). The expression pattern for Fbx2 correlates with the published binding pattern of the lectin ConA (Rueda et al, 2003), which, like Fbx2, specifically recognizes high-mannose N-linked glycoproteins. This concordant expression pattern for Fbx2 and ConA-detectable glycoproteins is consistent with a role for Fbx2 in glycoprotein homeostasis in the organ of Corti.…”

Section: Resultssupporting

confidence: 56%

Selective Cochlear Degeneration in Mice Lacking the F-Box Protein, Fbx2, a Glycoprotein-Specific Ubiquitin Ligase Subunit

et al. 2007

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Section: Resultssupporting

confidence: 56%

Selective Cochlear Degeneration in Mice Lacking the F-Box Protein, Fbx2, a Glycoprotein-Specific Ubiquitin Ligase Subunit

et al. 2007

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“…In another example using Concanavalin A, a lectin that reacts with high mannose-type glycans of glycoproteins, intense staining of the supporting cells and the tectorial membranes (TM) was observed. 48) Immunohistology of guinea pig cochlea tissue using a panel of 25 monoclonal antibodies directed to various carbohydrate epitopes identified lacto-series structures localized at specific sites of the TM and the organ of Corti. 49) …”

Section: Gangliosides In Auditory System and Hearing Lossmentioning

confidence: 99%

Physiopathological function of hematoside (GM3 ganglioside)

Inokuchi

2011

Proc. Jpn. Acad., Ser. B

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Since I was involved in the molecular cloning of GM3 synthase (SAT-I), which is the primary enzyme for the biosynthesis of gangliosides in 1998, my research group has been concentrating on our efforts to explore the physiological and pathological implications of gangliosides especially for GM3. During the course of study, we demonstrated the molecular pathogenesis of type 2 diabetes and insulin resistance focusing on the interaction between insulin receptor and gangliosides in membrane microdomains and propose a new concept: Life style-related diseases, such as type 2 diabetes, are a membrane microdomain disorder caused by aberrant expression of gangliosides. We also encountered an another interesting aspect indicating the indispensable role of gangliosides in auditory system. After careful behavioral examinations of SAT-I knockout mice, their hearing ability was seriously impaired with selective degeneration of the stereocilia of hair cells in the organ of Corti. This is the first observation demonstrating a direct link between gangliosides and hearing functions.

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“…As pointed out in several studies (Hilding et al 1977;Lim and Rueda 1990;Kawamata and Igarashi 1993;Prieto et al 1995;Bianchi et al 1999;Rueda et al 2003), pillar cells accumulate large amounts of polysaccharides. We therefore decided, subsequently, to apply the periodic acidthiocarbohydrazide-silver proteinate method (Thiery 1967), a cytochemical technique known to detect polysaccharides in biological material.…”

Section: Characterization Of First Identifiable Oc Cellmentioning

confidence: 90%

Early identification of inner pillar cells during rat cochlear development

et al. 2009

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Although the structure of the auditory organ in mature mammals, the organ of Corti, is clearly established, its development is far from being elucidated. Here, we examine its spatio-temporal development in rats from embryonic day 16 (E16) to E19 by using cytochemical and immunocytochemical methods at the light- and electron-microscope levels. We demonstrate that the organ of Corti develops from a non-proliferating cell zone that is located in the junctional region between two edges of the dorsal epithelium of the cochlear duct. We also reveal that the first cells to develop in this zone are the inner pillar cells, a particular type of non-sensory supporting cell, which arise in the base of the cochlear duct at the boundary between the two ridges at E16. Cell differentiation in this prosensory region continues according to a base-to-apex gradient; the inner hair cells appear in the greater epithelial ridge at E17 and the outer hair cells in the lesser epithelial ridge at E18. At E19, the various cell types of the organ of Corti are in place. Finally, we show that unlike the development of all the supporting cell types of the organ of Corti, the development of inner pillar cells within the prosensory domain seems not to involve Notch1 activation. These results highlight the central role that the inner pillar cells probably play in the development of the organ of Corti.

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Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Cited by 10 publications

References 58 publications

Selective Cochlear Degeneration in Mice Lacking the F-Box Protein, Fbx2, a Glycoprotein-Specific Ubiquitin Ligase Subunit

Selective Cochlear Degeneration in Mice Lacking the F-Box Protein, Fbx2, a Glycoprotein-Specific Ubiquitin Ligase Subunit

Physiopathological function of hematoside (GM3 ganglioside)

Early identification of inner pillar cells during rat cochlear development

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