Postnatal Appearance of Glycoconjugate with Terminal N-Acetylgalactosamine on the Surface of Selected Neurons in Mouse Brain

Nakagawa, Fukuo; Schulte, Bradley A.; Wu, Jang Yen; Spicer, Samuel S.

doi:10.1159/000111608

Cited by 26 publications

(11 citation statements)

References 10 publications

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“…The regional distribution and the time course of the appearance of PNs during postnatal development of the wild-type mice correspond with the data obtained in ICR mice by lectin staining (Nakagawa et al, 1987;Murakami et al, 1997) and in C3B6 mice by the detection of "glucuronic acid-and-sulphate-containing glycoproteins" (Yamamoto et al, 1988). The patterns also resembled those known from cytochemical studies in the rat (Watanabe et al, 1989;Fujita and Kudo, 1992;Seeger et al, 1994;Köppe et al, 1997a).…”

Section: Developmental Profiles Of Perineuronal Nets In Wild-type Anisupporting

confidence: 84%

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Postnatal development of perineuronal nets in wild-type mice and in a mutant deficient in tenascin-R

et al. 2000

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The extracellular matrix glycoprotein tenascin-R (TN-R), colocalizing with hyaluronan, phosphacan, and aggregating chondroitin sulphate proteoglycans in the white and grey matter, is accumulated in perineuronal nets that surround different types of neurons in many brain regions. To characterize the role of TN-R in the formation of perineuronal nets, we studied their postnatal development in wild-type mice and in a TN-R knock-out mutant by using the lectin Wisteria floribunda agglutinin and an antibody to nonspecified chondroitin sulphate proteoglycans as established cytochemical markers. We detected the matrix components TN-R, hyaluronan, phosphacan, neurocan, and brevican in the perineuronal nets of cortical and subcortical regions. In wild-type mice, lectin-stained, immature perineuronal nets were first seen on postnatal day 4 in the brainstem and on day 14 in the cerebral cortex. The staining intensity of these nets for TN-R, hyaluronan, phosphacan, neurocan, and brevican was extremely weak or not distinguishable from that of the surrounding neuropil. However, all markers showed an increase in staining intensity of perineuronal nets reaching maximal levels between postnatal days 21 and 40. In TN-R-deficient animals, the perineuronal nets tended to show a granular component within their lattice-like structure at early stages of development. Additionally, the staining intensity in perineuronal nets was reduced for brevican, extremely low for hyaluronan and neurocan, and virtually no immunoreactivity was detectable for phosphacan. The granular configuration of perineuronal nets became more predominant with advancing age of the mutant animals, indicating the continued abnormal aggregation of chondroitin sulphate proteoglycans complexed with hyaluronan. As shown by electron microscopy in the cerebral cortex, the disruption of perineuronal nets was not accompanied by apparent changes in the synaptic structure on net-bearing neurons. The regional distribution patterns and the temporal course of development of perineuronal nets were not obviously changed in the mutant. We conclude that the lack of TN-R initially and continuously disturbs the molecular scaffolding of extracellular matrix components in perineuronal nets. This may interfere with the development of the specific micromilieu of the ensheathed neurons and adjacent glial cells and may also permanently change their functional properties.

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Section: Developmental Profiles Of Perineuronal Nets In Wild-type Anisupporting

confidence: 84%

“…In mice, the first PNs in the cerebral cortex appear after the second postnatal week as shown by specific lectin staining. PNs associated with neurons in subcortical nuclei develop earlier (Nakagawa et al, 1987;Murakami et al, 1997).…”

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confidence: 99%

Postnatal development of perineuronal nets in wild-type mice and in a mutant deficient in tenascin-R

et al. 2000

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“…In a number of studies dealing with the structure and chemical nature of PNs it was shown that they represent accumulations of extracellular matrix composed of large aggregating chondroitin-sulphate proteoglycans (CSPGs) associated with hyaluronan (DeYoe et al 1990;Brauer et al 1993;Brückner et al 1993;Bertolotto et al 1996). These components have been cytochemically detected by the use of specific antibodies, lectins and basic dyes (Nakagawa et al 1987;Girard et al 1992;Härtig et al 1992;Brückner et al 1993Brückner et al , 1996aSchweizer at al. 1993;Celio and Blümcke 1994;Yasuhara et al 1994;Bertolotto et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Developmental patterns of proteoglycan-containing extracellular matrix in perineuronal nets and neuropil of the postnatal rat brain

Köppe

Brückner

Brauer

et al. 1997

Cell and Tissue Research

120

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The extracellular matrix is involved in various morphogenetic processes which are accompanied by changes in its physicochemical properties and spatial organization. In the adult brain it contributes to cellular communication and the regulation of neuronal activity. The present study deals with the postnatal appearance and transformation into adult distribution patterns of extracellular matrix components related to chondroitin-sulphate proteoglycans (CSPGs) in the rat brain. The differential accumulation of these components in neuropil and in perineuronal nets (PNs) enriched in certain regions was examined in 0-, 7-, 14-, 21- and 35-day-old rats and adult animals using the N-acetylgalactosamine-binding Wisteria floribunda agglutinin (WFA) and immunocytochemical detection of CSPGs. The lectin stained the olfactory-bulb glomerular layer and layer Ia of piriform and entorhinal cortex already in newborn animals. On postnatal day 7 diffuse neuropil staining was additionally found in certain subcortical nuclei and in deep neocortical layers. The first sharply contoured PNs were detected at this age in the brain stem, indicating the more advanced maturation of matrix components in subcortical regions. CSPG immunoreactivity yielded staining patterns largely identical to WFA-binding patterns but appeared only between postnatal day 14 and 21. The adult-like stage was revealed with both methods between 21 and 35 days after birth. The results provide further evidence that the accumulation of certain CSPGs in the extracellular space is spatiotemporally related to distinct patterns of neuronal activity at the regional and cellular level.

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“…In contrast to the situation in mammals, SBA labels the surface of only a (sub)population of olfactory neurones in eels (Franceschini and Ciani, 1991) and frogs (Hofmann and Meyer, 1991;Key and Giorgi, 1986), but does not label other cells in the central nervous system of these animals. In mice it has been shown that viciu uillosa agglutinin, which labels essentially the same cells as SBA (Kosaka and Heizmann, 19891, fails to produce the typical labelling pattern in 1 week old animals (Nakagawa et al, 1987). Adult-like labelling was observed by the age of 3 weeks.…”

Section: Discussionmentioning

confidence: 99%

The distribution of N-acetylgalactosamine in the cochlear nucleus of the gerbil revealed by lectin binding with soybean agglutinin

Gleich

1994

Hearing Research

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A horseradish peroxidase conjugated lectin from Glycine max (soy bean agglutinin; SBA) was used to characterise the distribution of N-acetylgalactosamine in the cochlear nucleus of the mongolian gerbil. SBA bound differentially to a variety of structures within the cochlear nucleus. Specific SBA labelling was associated with large non-granule neurones of variable size and shape throughout the cochlear nucleus. Compared to adjacent Nissl-stained sections 80% of the non-granule cells in the dorsal cochlear nucleus (DCN) and more than 90% of the non-granule cells in the ventral cochlear nucleus (VCN) bound SBA. The variation in location, size and shape as well as the high percentage of the labelled neurones suggest that cells of several, if not all, non-granule cell types, which have been described for the cochlear nucleus according to the usual Nissl schemes, are SBA positive. Granule cells did not bind SBA because all SBA-labelled cells had diameters above 10 pm. Diffuse labelling, not systematically associated with cells or fibres, was high in the molecular and fusiform cell layers of the DCN and that part of the granule cell area located close to the surface of the VCN. Darkly labelled granules (up to 2 pm diameter) were prominent in the area of the VIIIth nerve root. After long SBA incubations, they were also present in VCN and to a lesser degree in DCN. The results are discussed with respect to findings in other brain areas and the possible co-localisation of gamma aminobutyric acid (GABA), parvalbumin and N-acetylgalactosamine.

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Postnatal Appearance of Glycoconjugate with Terminal N-Acetylgalactosamine on the Surface of Selected Neurons in Mouse Brain

Cited by 26 publications

References 10 publications

Postnatal development of perineuronal nets in wild-type mice and in a mutant deficient in tenascin-R

Postnatal development of perineuronal nets in wild-type mice and in a mutant deficient in tenascin-R

Developmental patterns of proteoglycan-containing extracellular matrix in perineuronal nets and neuropil of the postnatal rat brain

The distribution of N-acetylgalactosamine in the cochlear nucleus of the gerbil revealed by lectin binding with soybean agglutinin

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