Histochemical detection of H2O2 by tissue printing as a precocious marker of rhizogenesis in grapevine

Neves, Clélia; Sá, M. Cláudia; Amâncio, Sara

doi:10.1016/s0981-9428(99)80019-9

Cited by 24 publications

(17 citation statements)

References 20 publications

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“…6, 7). The result was inconsistent with the earlier findings by Neves et al (1998), through histochemical detection of H 2 O 2 by a tissue-printing assay, they found that the meristemoids and afterwards the AR primordia shown low levels of H 2 O 2 in rhizogenesis of grapevine. This inconsistency was possibility caused by the difference of the used methods.…”

Section: Discussioncontrasting

confidence: 95%

Distribution of hydrogen peroxide during adventitious roots initiation and development in mung bean hypocotyls cuttings

et al. 2010

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Previous studies have shown that hydrogen peroxide (H 2 O 2 ) may mediate the auxin response during the formation of adventitious roots (AR). However, the mechanism and distribution of H 2 O 2 during AR formation remains unclear. In this study, we investigate the spatiotemporal changes and role of H 2 O 2 in AR initiation and development. Application of 5-100 mM H 2 O 2 to Mung bean (Phaseolus radiatus L.) hypocotyl cuttings induced AR formation in a dose-dependent manner. The effect was blocked by ascorbic acid (AA), an important reducing substrate for H 2 O 2 reduction. Depletion of endogenous H 2 O 2 by AA resulted in the significant reduction of AR emergence, suggesting a physiological role for H 2 O 2 in the regulation of AR formation. Determination of H 2 O 2 content showed that the level of H 2 O 2 increased gradually and reached the highest value 60 h after induction of AR. Further detection of endogenous H 2 O 2 by the specific fluorescent probe dichlorofluorescein diacetate (H 2 DCF-DA) and 3,3 0 -diaminobenzidine (DAB) staining in transverse sections of the basal region of cuttings revealed that obvious H 2 O 2 signals were observed in the pericycle cells between the vascular bundles 24 h after the primary roots were removed. With the development of root primordia, H 2 O 2 signals increased gradually and were mainly distributed in the root meristem. AA significant inhibited the H 2 O 2 -dependent fluorescence and the formation of AR, suggesting an essential role of H 2 O 2 generation during AR initiation and development. Furthermore, the involvement of Ca 2? during H 2 O 2 -mediated AR formation was evaluated. Ca 2? channel inhibitors LaCl 3 and ruthenium red (RR) and Ca 2? chelator ethylene glycol-bis(2-aminoethylether)-N,N,N 0 ,N 0 -tetraacetic acid (EGTA) prevent H 2 O 2 -induced AR formation, which indicate that the hypocotyl cuttings response to H 2 O 2 depends on the availability of both intracellular and extracellular Ca 2? pools, and Ca 2? is a downstream messenger in the signaling pathway triggered by H 2 O 2 to promote adventitious root formation.

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Section: Discussioncontrasting

confidence: 95%

Distribution of hydrogen peroxide during adventitious roots initiation and development in mung bean hypocotyls cuttings

et al. 2010

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“…This coincides with the protrusion of new roots and the expansion of the first ex vitro leaf and culminates with a peak of ABA and H 2 O 2 concentration on the seventh day after transfer, both produced in the newly-expanded and functional roots (Figure 3; Neves et al, 1998;Vilela et al, 2007). At this point, acclimatization to ex vitro is not yet complete but photooxidative stress is no longer a problem for the growing grapevine plants.…”

Section: Oxidative Stress In In Vitro Systemsmentioning

confidence: 80%

Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Carvalho

Vidigal

Amâncio

2015

Front. Environ. Sci.

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Plants can maintain growth and reproductive success by sensing changes in the environment and reacting through mechanisms at molecular, cellular, physiological, and developmental levels. Each stress condition prompts a unique response although some overlap between the reactions to abiotic stress (drought, heat, cold, salt or high light) and to biotic stress (pathogens) does occur. A common feature in the response to all stresses is the onset of oxidative stress, through the production of reactive oxygen species (ROS). As hydrogen peroxide and superoxide are involved in stress signaling, a tight control in ROS homeostasis requires a delicate balance of systems involved in their generation and degradation. If the plant lacks the capacity to generate scavenging potential, this can ultimately lead to death. In grapevine, antioxidant homeostasis can be considered at whole plant levels and during the development cycle. The most striking example lies in berries and their derivatives, such as wine, with nutraceutical properties associated with their antioxidant capacity. Antioxidant homeostasis is tightly regulated in leaves, assuring a positive balance between photosynthesis and respiration, explaining the tolerance of many grapevine varieties to extreme environments. In this review we will focus on antioxidant metabolites, antioxidant enzymes, transcriptional regulation and cross-talk with hormones prompted by abiotic stress conditions. We will also discuss three situations that require specific homeostasis balance: biotic stress, the oxidative burst in berries at veraison and in vitro systems. The genetic plasticity of the antioxidant homeostasis response put in evidence by the different levels of tolerance to stress presented by grapevine varieties will be addressed. The gathered information is relevant to foster varietal adaptation to impending climate changes, to assist breeders in choosing the more adapted varieties and suitable viticulture practices.

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“…This staining identifies a small degree of lignification in blue, increasing lignification in pink and tannins in red. For the tissue printing of H 2 O 2 , the printing membrane was prepared as described in Neves et al (1998). Briefly, bands of nitrocellulose (0.2 lm) were soaked with a solution containing 100 g l -1 soluble starch and 0.5 mol l -1 KI in distilled water, dried in an air stream at 30°C and stored in the dark.…”

Section: Histology and Tissue Printingmentioning

confidence: 99%

“…Experiments were repeated at least twice with similar results. Calibration of the H 2 O 2 printing assay and specificity of the test reaction for H 2 O 2 was as described in Neves et al (1998).…”

Section: Histology and Tissue Printingmentioning

confidence: 99%

“…Hydrogen peroxide (H 2 O 2 ) is a substrate for peroxidases in the formation of cell wall polymers such as lignin (Boerjan et al 2003) and insoluble glycoproteins (Jackson et al 2001). The histological modifications induced by auxin treatments on root induction and formation can be made evident by the technique of tissue printing for H 2 O 2 (Neves et al 1998).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Patterns of B-type cyclin gene expression during adventitious rooting of micropropagated cork oak

Neves

Hand

Amâncio³

2006

Plant Cell Tiss Organ Cult

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The aim of our study is the identification of the critical points in the rooting process at the molecular level for possible improvement of the propagation of cork oak. The rhizogenic potential of woody plants depends on both genotype and developmental stage. To investigate the role of cyclin transcription in the regulation of cell division during adventitious root induction, we have analysed the temporal expression of a member of the cyclin gene family. The histological modifications that occur during the root induction and formation processes as a consequence of auxin treatments can be detected by the technique of tissue printing for hydrogen peroxide (H 2 O 2 ). Thus a H 2 O 2 printing assay was performed in parallel with cyclin expression studies. We report the cloning and sequencing of a B-type cyclin genomic fragment from micropropagated cork oak using gene-specific and genotype-independent oligonucleotide primers to conserved regions of the gene. After 48 h of auxin induction, the expression of this B-type cyclin increased, possibly in association with the first cell divisions. The expression observed in control shoots may reflect unorganised cell divisions associated with callus formation.

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Histochemical detection of H2O2 by tissue printing as a precocious marker of rhizogenesis in grapevine

Cited by 24 publications

References 20 publications

Distribution of hydrogen peroxide during adventitious roots initiation and development in mung bean hypocotyls cuttings

Distribution of hydrogen peroxide during adventitious roots initiation and development in mung bean hypocotyls cuttings

Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Patterns of B-type cyclin gene expression during adventitious rooting of micropropagated cork oak

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