Patterns of B-type cyclin gene expression during adventitious rooting of micropropagated cork oak

Abstract

The aim of our study is the identification of the critical points in the rooting process at the molecular level for possible improvement of the propagation of cork oak. The rhizogenic potential of woody plants depends on both genotype and developmental stage. To investigate the role of cyclin transcription in the regulation of cell division during adventitious root induction, we have analysed the temporal expression of a member of the cyclin gene family. The histological modifications that occur during the root induction and formation processes as a consequence of auxin treatments can be detected by the technique of tissue printing for hydrogen peroxide (H 2 O 2 ). Thus a H 2 O 2 printing assay was performed in parallel with cyclin expression studies. We report the cloning and sequencing of a B-type cyclin genomic fragment from micropropagated cork oak using gene-specific and genotype-independent oligonucleotide primers to conserved regions of the gene. After 48 h of auxin induction, the expression of this B-type cyclin increased, possibly in association with the first cell divisions. The expression observed in control shoots may reflect unorganised cell divisions associated with callus formation.

Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation

“…Regarding the role of auxin in cell division and elongation, a complex interaction between auxin, cytokinin, cyclins and cyclin-dependent kinases (CDKs) governs the plant cell cycle (Hartig and Beck 2006; Komaki and Sugimoto 2012) Auxin treatment stimulated expression of cyclins including mitotic B1 cyclins in Arabidopsis (Ferreira et al 1994; Richard et al 2002), and expression of cyclin genes and of CDKs has already been related to auxin-induced AR formation (Lindroth et al 2001; Neves et al 2006). When Petunia cuttings experienced the same rooting conditions as applied to the controls in the present study, the transcript of a Petunia CycB1 encoding a mitotic B1 cyclin accumulated in the stem base at 48 hpe (Ahkami et al 2009).…”

Plant Hormone Homeostasis, Signaling, and Function during Adventitious Root Formation in Cuttings

“…Cyclin-dependent kinases and cyclins as their regulatory proteins are involved in auxin- and cytokinin-mediated governing of the cell cycle (Hartig and Beck, 2006 ; Komaki and Sugimoto, 2012 ). Expression of cyclin genes has already been related to auxin-induced AR formation in Pinus and Quercus (Lindroth et al, 2001 ; Neves et al, 2006 ). In petunia cuttings, transcripts coding for Cyclin B1 and Cyclin D accumulated from 2 and 4 dpe onwards in the stem base, respectively (Ahkami et al, 2009 , 2014 ).…”

Transcriptome Dynamics of Rooting Zone and Leaves during In Vitro Adventitious Root Formation in Eucalyptus nitens

“…Cyclins, cyclin-dependent kinases, and their regulatory proteins are involved in auxin- and cytokinin-mediated cell cycle control [ 70 ]. Researchers have related the gene expression of cyclins, induced by auxins, to the formation of adventitious roots in Pinus and Quercus [ 71 , 72 ]. Cyclin B1 and Cyclin D transcriptions increase at the Petunia stem base from 2 to 4 days after cutting [ 73 ]; Similarly, the expressions of Cyclin D (T67, T68) and the cyclin-dependent kinases CKB1-1 (T69, T70), CKS1 (T71), and CDKA1 (T72) increased in the rooting zone of E. nitens over a similar incubation period.…”