Histochemical and Immunohistochemical Study of Diffuse Large-cell Lymphomas

Li, Chin-Yang; Harrison, Edgar G.

doi:10.1093/ajcp/70.5.721

Cited by 50 publications

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“…Whether the difference in CD19 expression represents this difference in technique or true difference in tumor cell antigen expression is not clear at this time. The Ig heavy chain isotype of the CNS lymphomas closely parallels that of non-CNS DLCLs with most cases expressing IgM, and less commonly IgG [19][20][21][22][23][24]. Therefore, with the exception of CD19 the expression of pan B cell antigens on primary and secondary CNS large cell lymphomas is generally similar to that of non-CNS DLCLs.…”

Section: Discussionmentioning

confidence: 99%

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Immunophenotypic profile of CNS lymphoma: A review of eighteen cases

et al. 1989

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The cell surface antigenic phenotype of 18 cases of central nervous system (CNS) large-cell lymphoma (14 primary, four secondary) was examined by an immunoperoxidase technique using antibodies that identify B cell restricted and associated antigens. All cases were shown to be of B cell origin by virtue of the expression of monotypic immunoglobulin (Ig) (16 IgM, two IgG) and the pan B cell antigen B1 (CD20). A panel of monoclonal antibodies directed against B cell restricted and associated activation antigens including B5, Blast-1, Blast-2 (CD23), BB1, interleukin 2 receptor (IL2R, CD25), T9 (transferrin receptor) and TNK-TAR (4F2) was used on 12 of the cases. The majority expressed T9 and TNK-TAR. Blast-1 was expressed by less than half the cases and Blast-2 and B5 by one of 12 cases each. This is in contrast to 10 non-CNS diffuse large cell lymphomas where B5 and Blast-1 were present on all cases. This study confirms previous observations that primary CNS large cell lymphomas are of B cell derivation. Moreover, the differences in expression of B cell activation antigens on CNS large cell lymphomas as compared to non-CNS lymphomas raise the possibility that a subset of neoplastic B cells may have unique tropism for the CNS.

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Section: Discussionmentioning

confidence: 99%

“…A number of recent studies have characterized the cell surface phenotype of non-CNS diffuse large cell lymphoma [DLCL] [19,25]. The majority of these cases are shown to be of B cell origin by virtue of the expression of the B cell restricted antigens Ig and/or B1 [CD20] [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Immunophenotypic profile of CNS lymphoma: A review of eighteen cases

et al. 1989

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“…The plasmacytic features evident in most of the cells are rough endoplasmic reticulum which is sparse and not well organized in lamella as is frequently observed in non-secretory cells or as abundant in more highly anaplastic cells. The Golgi area and prominent, often bizarre mitochondria, dense osmiophilic bodies, and vacuoles are strikingly evident, as are the ovoid, eccentric nuclei with peripheral chromatin distribution, Histochemical staining revealed that the cells were and continue to be myeloperioxidase- (Graham and Karnovsky, 1966), sudan black- (Li and Harrison, 1978), albumin-and lysozyme- (McManus, 1946) negative. A few cells were noted by us to be clearly PAS- (Sheehan and Storey, 1947) positive but two observers have reported the cells to be negative or equivocal (+).…”

Section: Morphologymentioning

confidence: 97%

Establishment and characterization of a human non‐secretory plasmacytoid cell line and its hybridization with human B cells

Ritts

Ruı́z-Argüelles

Weyl

et al. 1983

Intl Journal of Cancer

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A human non-secretory plasmacytoid cell line has been established for 6 years in more than 170 passages. Over 300 passages have been made from several early and late passages. The cell line is karyotypically normal, easily grown and has the characteristic features of a non-secretory plasmablast. Its characteristics suggest its use for hybridization by new methods as well as a study of its secretory defect. HPRT-negative phenotypic mutants can be derived from this line and a single polyploid clone has also been isolated. Hybridization with the HPRT+ and HPRT- lines X human B cells is described.

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“…The unlabeled antibody enzyme method of Sternberger et al I4-l5 using horseradish peroxidase-antiperoxidase (PAP) complex as the indicator was used for the study, with minor modification as previously de-scribed. 6 The immunoperoxidase reaction was performed as follows:…”

Section: Immunoperoxidase Stainsmentioning

confidence: 99%

Immunoperoxidase staining of bone marrow sections

Hitzman

Kyle

1981

Cancer

Self Cite

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Paraffin-embedded sections of bone marrow from 131 patients were studied. The specificity of the immunoperoxidase technique for detecting intracellular kappa and lambda light chains of immunoglobulin was first evaluated in 34 cases of typical multiple myeloma or macroglobulinemia. The monoclonal light chain detected in the bone marrow myeloma was identical to the result of immunoelectrophoresis of serum or urine. By use of the same method, 97 cases in which the diagnosis was difficult were examined; cases included those of bone marrow plasmacytosis, lymphocytosis, and morphologically unusual cells. All marrow cells with monoclonal immunoglobulin detected by immunoperoxidase stain eventually proved to be a B-cell neoplastic or potentially neoplastic process. This study confirms the specificity of the immunoperoxidase stain and demonstrates its value in solving clinical diagnostic problems, that is, the diagnosis of nonsecretory myeloma and unusual morphology, differentiating the monoclonal plasmaproliferative disorders from reactive plasmacytosis or lymphoma from lymphocytosis.

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Histochemical and Immunohistochemical Study of Diffuse Large-cell Lymphomas

Cited by 50 publications

References 0 publications

Immunophenotypic profile of CNS lymphoma: A review of eighteen cases

Immunophenotypic profile of CNS lymphoma: A review of eighteen cases

Establishment and characterization of a human non‐secretory plasmacytoid cell line and its hybridization with human B cells

Immunoperoxidase staining of bone marrow sections

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