2021
DOI: 10.3390/ijms22041785
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hiPSC-Derived Epidermal Keratinocytes from Ichthyosis Patients Show Altered Expression of Cornification Markers

Abstract: Inherited ichthyoses represent a large heterogeneous group of skin disorders characterised by impaired epidermal barrier function and disturbed cornification. Current knowledge about disease mechanisms has been uncovered mainly through the use of mouse models or human skin organotypic models. However, most mouse lines suffer from severe epidermal barrier defects causing neonatal death and human keratinocytes have very limited proliferation ability in vitro. Therefore, the development of disease models based on… Show more

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Cited by 5 publications
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“…LSCs and primary keratinocytes were grown in cell culture chamber slides (Corning, New York, USA), and, when confluent, fixed using 4% PFA solution. Immunostainings were performed as previously described [ 97 ]. Briefly, cells were washed, permeabilized for 15 min with 0.1% Triton X-100, washed again, and blocked for 2 h in 1% FBS.…”
Section: Methodsmentioning
confidence: 99%
“…LSCs and primary keratinocytes were grown in cell culture chamber slides (Corning, New York, USA), and, when confluent, fixed using 4% PFA solution. Immunostainings were performed as previously described [ 97 ]. Briefly, cells were washed, permeabilized for 15 min with 0.1% Triton X-100, washed again, and blocked for 2 h in 1% FBS.…”
Section: Methodsmentioning
confidence: 99%