Highly sensitive electrochemiluminescenc assay of acetylcholinesterase activity based on dual biomarkers using Pd–Au nanowires as immobilization platform

Cui, Yun; Wang, Minqiang; Zhong, Xia; Chen, Shihong; Chai, Yaqin; Yuan, Ruo

doi:10.1016/j.bios.2015.11.096

Cited by 65 publications

(19 citation statements)

References 24 publications

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“…Synthesis of ethyl 1-(2-hydroxybutyl)-4-methyl-6-phenyl-2-thioxo-1,2,3,6-tetrahydropyrimidine-5-carboxylate (3) Ethyl 6-methyl-2-thioxo-4-(p-tolyl)-1,2,3,4-tetrahydropyrimidine-5-carboxylate (1.52 g, 0.02 mol) is dissolved in 2:1 ratio of acetylacetone and ethyl alcohol (12 mL:5 mL) and 1,2-epoxobutane (2.03 mL, 0.02 mol) is added on it drop by drop. After being dissolved in the stirrer for 30 min, 0.02 g AlCl 3 catalyst is added on it and mixed by heating at [60][61][62][63][64][65] C. …”

Section: Chemistrymentioning

confidence: 99%

“…Both cholinesterases participate in cholinergic neurotransmission by hydrolysing ACh in the central and peripheral nervous system. Also, they play an important role in the development of Alzheimer's disease (AD) 65 . AD is a neurodegenerative disease of the central nervous system associated with progressive memory loss resulting in dementia 66 .…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of some tetrahydropyrimidine-5-carboxylates, determination of their metal chelating effects and inhibition profiles against acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase

Sujayev

Garibov

Taslimi

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

105

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2-(Methacryloyloxy)ethyl 6-methyl-2-oxo-4-phenyl-1,2,3,4-tetrahydropyrimidine-5-carboxylate, is a cyclic urea derivative synthesized from urea, 2-(methacryloyloxy) ethyl acetoacetate and substituted benzaldehyde, and tested in terms of the inhibition of two physiologically relevant carbonic anhydrase (CA) isozymes I and II. Acetylcholinesterase (AChE) is found in high concentrations in the red blood cells and brain. Butyrylcholinesterase (BChE) is another enzyme abundantly present in the liver and released into blood in a soluble form. Also, they were tested for the inhibition of

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Section: Chemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis of some tetrahydropyrimidine-5-carboxylates, determination of their metal chelating effects and inhibition profiles against acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase

Sujayev

Garibov

Taslimi

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

105

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“…Recently two biosensors for AChE have been developed and applied to biological matrices [64,65]. An ECL biosensor modified with Pd-Au nanowires detected AChE in blood samples [64]. The Au-Pd nanowires increased the surface area for the immobilization of the bioenzyme AChE and choline oxidase, while sustaining signal amplification by providing catalytic activity of [66,72].…”

Section: Acetylcholinesterase Detectionmentioning

confidence: 99%

“…This aggregation is often associated with the development of Alzheimer's disease. Recently two biosensors for AChE have been developed and applied to biological matrices [64,65]. An ECL biosensor modified with Pd-Au nanowires detected AChE in blood samples [64].…”

Section: Acetylcholinesterase Detectionmentioning

confidence: 99%

“…Normal human serum sample 50 nM 0.5-600 μM [61] Glucose Signal enhancement at electrode modified with Pt nanoflowers/ graphene oxide/glucose oxidase Blood serum 2.8 μM 5-80 μM; 80-1000 μM [62] Glucose Perovskite LaTiO 3 -Ag 0.1 nanocomposite (catalytic oxidation of glucose) Blood 2.50 nM 0.01 μM-0.10 mM [63] Acetylcholinesterase Biosensor modified with Pd-Au nanowires Human blood serum samples 0.0083 U/l 0.025 U/l-25 KU/l [64] Acetylcholinesterase Graphene-Au-chitosan nanocomposite electrode with biofunctional biocomposite Human serum 2.2 nM 6.7 nM-0.92 mM [65] Protein kinase Signal amplification from AuNPs with Xanthine oxidase 10% fetal bovine serum 0.09 U/ml 0.1-10 U/ml [66] Amyloid B Immunosensor with luminolmodified Ce-ZnO nanoflowers Diluted serum 52 fg/ml 80 fg/ml-100 ng/ml [67] Cholesterol Cathodic ECL at 3D-MoS 2 polyaniline nanoflowers with Ag nanocubes loaded with cholesterol oxidase Diluted human serum 1.1 nM 3.3 nM-0.45 mM A review of electrogenerated chemiluminescent biosensors for assays in biological matrices Review important role in the regulation of cell growth and division. PDGF-BB, specifically, is a good predictor of DN.…”

Section: Platelet-derived Growth Factormentioning

confidence: 99%

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A Review of Electrogenerated Chemiluminescent Biosensors for Assays in Biological Matrices

2016

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Electrogenerated chemiluminescence (ECL) is the production of light via electron transfer reactions between electrochemically produced reagents. ECL-based biosensors use specific biological interactions to recognize an analyte and produce a luminescent signal. Biosensors fabricated with novel biorecognition species have increased the number of analytes detected. Some of these analytes include peptides, cells, enzymes and nucleic acids. ECL biosensors are selective, simple, sensitive and have low detection limits. Traditional methods use ruthenium complexes or luminol to generate ECL. Nanomaterials can be incorporated into ECL biosensors to improve efficiency, but also represent a new class of ECL emitters. This article reviews the application of ruthenium complex, luminol and nanomaterial-based ECL biosensors to making measurements in biological matrices over the past 4 years.

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Synthesis and Carbonic Anhydrase Inhibition of Tetrabromo Chalcone Derivatives

Koçyiğit

Budak

Eligüzel

et al. 2017

Archiv der Pharmazie

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In the present study, human carbonic anhydrase (hCA) enzyme was purified and characterized from fresh blood human red cells by Sepharose-4B-l-tyrosine-sulfanilamide affinity gel chromatography. Secondly, a series of new tetrabromo chalcone derivatives containing 4,7-methanoisoindol-1,3-dione (2a-i) were synthesized from the addition of Br to related chalcone derivatives (1a-i). The structures of the new molecules (2a-i) were confirmed by means of H NMR, C NMR and elemental analysis. Finally, the inhibitory effects of 2a-i on CA activities were investigated using the esterase method under in vitro conditions. The compounds 2a-i exhibited excellent inhibitory effects, in the low nanomolar range, with K values in the range of 11.30-21.22 nM against hCA I and in the range of 8.21-12.86 nM against hCA II. Our findings suggest that the new compounds 2a-i have superior inhibitory effect over acetazolamide (AZA), which is used as clinical CA inhibitor, with obtained K values of 34.50 and 28.93 nM against the hCA I and II isozymes, respectively. In addition to the inhibition assays, molecular modeling approaches were implemented for prediction of the binding affinities of compounds 2a and 2c, which had the highest inhibition effects, against the hCA I and II isozymes.

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Highly sensitive electrochemiluminescenc assay of acetylcholinesterase activity based on dual biomarkers using Pd–Au nanowires as immobilization platform

Cited by 65 publications

References 24 publications

Synthesis of some tetrahydropyrimidine-5-carboxylates, determination of their metal chelating effects and inhibition profiles against acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase

Synthesis of some tetrahydropyrimidine-5-carboxylates, determination of their metal chelating effects and inhibition profiles against acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase

A Review of Electrogenerated Chemiluminescent Biosensors for Assays in Biological Matrices

Synthesis and Carbonic Anhydrase Inhibition of Tetrabromo Chalcone Derivatives

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