Highly-sensitive detection of eight typical fluoroquinolone antibiotics by capillary electrophoresis-mass spectroscopy coupled with immunoaffinity extraction

Zhang, Xiao-Hui; Deng, Yan; Zhao, Mi; Zhou, Ying‐Lin; Zhang, Xin‐Xiang

doi:10.1039/c7ra12557g

Cited by 31 publications

(16 citation statements)

References 44 publications

(28 reference statements)

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“…Fluoroquinolones belong to a synthetic group of antibiotics, originated from quinolone nalidixic acid by the addition of a fluorine atom at carbon 6 and piperazine at carbon 7 position [ 108 ]. FQs kill bacteria or inhibit their growth by inhibiting their DNA replication favored by their chemical structure [ 109 ] ( Table 1 ).…”

Section: Gold Nanoparticles (Aunps) Based Colorimetric Aptasensorsmentioning

confidence: 99%

Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade

Zahra

Ali

et al. 2021

Nanomaterials

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Misuse of antibiotics has recently been considered a global issue because of its harmful effects on human health. Since conventional methods have numerous limitations, it is necessary to develop fast, simple, sensitive, and reproducible methods for the detection of antibiotics. Among numerous recently developed methods, aptasensors are fascinating because of their good specificity, sensitivity and selectivity. These kinds of biosensors combining aptamer with colorimetric applications of gold nanoparticles to recognize small molecules are becoming more popular owing to their advantageous features, for example, low cost, ease of use, on-site analysis ability using naked eye and no prerequisite for modern equipment. In this review, we have highlighted the recent advances and working principle of gold nanoparticles based colorimetric aptasensors as promising methods for antibiotics detection in different food and environmental samples (2011–2020). Furthermore, possible advantages and disadvantages have also been summarized for these methods. Finally, the recent challenges, outlook, and promising future perspectives for developing novel aptasensors are also considered.

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Section: Gold Nanoparticles (Aunps) Based Colorimetric Aptasensorsmentioning

confidence: 99%

Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade

Zahra

Ali

et al. 2021

Nanomaterials

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“…Zhang et al. presented a method for the determination of eight FQs in lake water by CZE‐MS with preliminary offline immunoaffinity extraction (IAE) for sample enrichment . The use of a polyclonal antibody for IAE enabled the simultaneous detection of all eight FQs with LODs from 1.2 to 5.0 nM.…”

Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables molecular characterization based on fragmentation. Although hyphenation of CE and MS is not straightforward, much emphasis has been placed on enabling efficient ionization and user‐friendly coupling. Though several interfaces are now commercially available, research on more efficient and robust interfacing with nano‐electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI) and inductively coupled plasma mass spectrometry (ICP) continues with considerable results. At the same time, CE‐MS has been used in many fields, predominantly for the analysis of proteins, peptides and metabolites. This review belongs to a series of regularly published articles, summarizing 248 articles covering the time between June 2016 and May 2018. Latest developments on hyphenation of CE with MS as well as instrumental developments such as two‐dimensional separation systems with MS detection are mentioned. Furthermore, applications of various CE‐modes including capillary zone electrophoresis (CZE), nonaqueous capillary electrophoresis (NACE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF) coupled to MS in biological, pharmaceutical and environmental research are summarized.

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“…An example of evaluation of this breakthrough volume is given in Figure 2 A which illustrates the effect of the volume of sample percolated on an anti-ciprofloxaxin (CIP) IS (2 mL gel) on the recovery of extraction of CIP. The affinity of the anti-CIP antibodies allows the percolation of up to 5.5 mL of sample while the recovery of quinine (QUIN), not recognized by these antibodies, decreases from 2 mL of sample (33). The optimization of an extraction procedure also consists in applying strong elution conditions to limit the volume of eluent, as illustrated by the elution of CIP with different ratios of methanol in water (Figure 2 B), thus favoring high enrichment factors without requiring in some cases additional evaporation steps of the final extract.…”

Section: -1-spe In Cartridge or Columnmentioning

confidence: 99%

Aptamer-based and immunosorbents

Pichon

2020

Solid-Phase Extraction

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To face the analysis of compounds that may be present at very low levels of concentration in complex media, such as food, environmental and biological fluids, it is possible to exploit the affinity and specificity of antibodies for their antigen by immobilizing them on a solid support. The resulting immunosorbents allow an effective extraction of compounds of interest while eliminating the other components of the matrix. The objective is then to make the analysis more reliable in terms of quantifying target analytes by eliminating matrix effects. A similar potential can also be obtained using aptamers, i.e. DNA or RNA sequences with high specificity towards compounds. The objective of this chapter is to present the respective characteristics of immunosorbents and aptamer-based sorbents, also known as oligosorbents, and their potential for the selective extraction of targeted compounds for the treatment of real samples.

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Highly-sensitive detection of eight typical fluoroquinolone antibiotics by capillary electrophoresis-mass spectroscopy coupled with immunoaffinity extraction

Abstract: An off-line procedure, immunoaffinity extraction (IAE), followed by capillary electrophoresis-mass spectroscopy (CE-MS) has been developed for the simultaneous determination of eight typical FQs in environmental water samples.

Cited by 31 publications

References 44 publications

Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade

Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Aptamer-based and immunosorbents

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