Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade

Abstract

Misuse of antibiotics has recently been considered a global issue because of its harmful effects on human health. Since conventional methods have numerous limitations, it is necessary to develop fast, simple, sensitive, and reproducible methods for the detection of antibiotics. Among numerous recently developed methods, aptasensors are fascinating because of their good specificity, sensitivity and selectivity. These kinds of biosensors combining aptamer with colorimetric applications of gold nanoparticles to recognize small molecules are becoming more popular owing to their advantageous features, for example, low cost, ease of use, on-site analysis ability using naked eye and no prerequisite for modern equipment. In this review, we have highlighted the recent advances and working principle of gold nanoparticles based colorimetric aptasensors as promising methods for antibiotics detection in different food and environmental samples (2011–2020). Furthermore, possible advantages and disadvantages have also been summarized for these methods. Finally, the recent challenges, outlook, and promising future perspectives for developing novel aptasensors are also considered.

A Simple Yet Effective Preanalytical Strategy Enabling the Application of Aptamer-Conjugated Gold Nanoparticles for the Colorimetric Detection of Antibiotic Residues in Raw Milk

“…A promising class of bioreceptors is DNA aptamers, which consist of short single-stranded oligonucleotides that provide high affinity and specificity for non-nucleotide molecules, including those with low molecular weight, toxic or nonimmunogenic [ 20 ]. The combination of AuNPs with aptamers in nanoaptasensors (NAS) is widely used to investigate the detection of a variety of analytes [ 21 , 22 , 23 , 24 ], with a growing number of reports addressing the detection of antibiotics in the field of food safety [ 25 , 26 ]. Surface plasmon resonance (SPR) is an optical property of AuNPs that allows the development of label-free NAS capable of analyzing multiple analytes in real time [ 27 ].…”

Optical biosensors - Illuminating the path to personalized drug dosing

“…The above example demonstrates how small molecules, while affordable and robust, do not provide a sufficient degree of selectivity for practical clinical use. Consequently, biologically derive constructs, such as antibodies, enzymes, and aptamers, have been explored as highly selective and sensitive bioreceptors (Zahra et al, 2021). For example, gold nanoparticles (AuNPs) were employed in conjunction with digoxin aptamers for the quantification of digoxin (Sarreshtehdar Emrani et al, 2015).…”

Gold Tablets: Gold Nanoparticles Encapsulated into Dextran Tablets and Their pH-Responsive Behavior as an Easy-to-Use Platform for Multipurpose Applications

“…For example, aptamer wrapped AuNPs have been reported for the detection of T2-toxin in wheat and corn, 23 aflatoxins in milk, 24 , 25 and antibiotics in many food and environmental samples. 26 Recently, Zha et al reported a dual-modal immunosensor based on biotin-labeled IgG-modified AuNPs for the detection of chloroacetamide herbicides. 27 Also, sodium malonate capped AuNPs were reported for the detection of barium ions in gunshot residues as a potential application in forensic sciences.…”