Highly sensitive and selective nanostructured microbiosensors for glucose and lactate simultaneous measurements in blood serum and in vivo in brain tissue

Fabrication of a Lactate-Specific Molecularly Imprinted Polymer toward Disease Detection

“…In the further development and integration of these MIP powders into devices, the analytical performance has been compared to current lactate detection devices (not all utilizing MIP detection) as shown in Table . Regiart et al designed an enzyme-based sensor with a wide linear range and high sensitivity toward the simultaneous detection of lactate and glucose . Booth et al also developed a sensitive sensor for neurometabolic lactate monitoring, highlighting possible applications of fiber-based biosensors for direct monitoring of brain metabolites in the context of injury, where miniaturization is required .…”

“…Regiart et al designed an enzyme-based sensor with a wide linear range and high sensitivity toward the simultaneous detection of lactate and glucose. 8 Booth et al also developed a sensitive sensor for neurometabolic lactate monitoring, highlighting possible applications of fiber-based biosensors for direct monitoring of brain metabolites in the context of injury, where miniaturization is required. 10 Although some of the devices report a wider linear range and lower sensitivities, their concentration levels are not in the specific range we are targeting that can indicate infection.…”

“…Equipment of this caliber is expensive, requires training to operate, uses invasive collection methods, and needs large sample volumes and long sample-processing times, making them unsuitable for diagnosis/treatment, particularly at the point-of-care (POC) . At present, amperometric meters have been developed for lactate biomarker analysis sampled from blood serum, sweat, and brain and peripheral tissues. − These devices are reliant on enzyme species, which can often suffer from poor physicochemical stability (e.g., pH and temperature changes), resulting in degradation and eventually denaturation, whereby enzyme characteristics cannot be recovered. Moreover, the inadequate shelf life of these species can have detrimental effects on the long-term sensing capabilities of these devices.…”

In Vivo Electrochemical Biosensors: Recent Advances in Molecular Design, Electrode Materials, and Electrochemical Devices

“…8 Regiart et al developed a highly sensitive and selective nanostructured lactate and glucose microbiosensor by modifying with nanoporous gold and PtNPs (Figure 4D). 128 PtNPs were electrodeposited on nanoporous gold films, which enhanced the sensitivity and the electrocatalytic properties toward H 2 O 2 detection. The usefulness of the microbiosensors was validated by the simultaneous determination of lactate and glucose in blood serum samples and the basal extracellular concentrations in four different rat brain structures.…”

“…Platinum nanoparticles (PtNPs) have excellent electrocatalytic activity for H 2 O 2 and are attractive for in vivo applications due to their size and shape dependent electronic properties . Regiart et al developed a highly sensitive and selective nanostructured lactate and glucose microbiosensor by modifying with nanoporous gold and PtNPs (Figure D) . PtNPs were electrodeposited on nanoporous gold films, which enhanced the sensitivity and the electrocatalytic properties toward H 2 O 2 detection.…”