Highly reproducible capillary gel electrophoresis (CGE) of DNA fragments using uncoated columns. Detection of genetically modified maize by PCR-cGE

García‐Cañas, Virginia; González, R. N.; Cifuentes, Alejandro

doi:10.1002/1615-9314(20020601)25:9<577::aid-jssc577>3.0.co;2-7

Cited by 40 publications

(22 citation statements)

References 26 publications

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“…Following the first works published on GMOs analysis by PCR-CGE-LIF [71][72][73][74][75] some new applications have come out also on this topic. Thus, a PCR-CGE-LIF method for GMO detection involved the use of fluorescent labeled primers with different dyes to perform the simultaneous PCR amplification in a single tube [62].…”

mentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes¹

2006

Electrophoresis

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150

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confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes¹

2006

Electrophoresis

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150

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“…[48][49][50] Capillary electrophoresis applied to GMO detection has the advantages of a high degree of automation; with on-line detection systems, minimal requirement of sample and reagents, and very efficient PCR product separations. 51,52 Most of these alternative detection methods could be especially useful in combination with the QC-PCR methods described below. …”

Section: End-point Detection Of Pcr Productsmentioning

confidence: 99%

“…59 The application of onestep, more quantitative methods will probably help to solve these problems in the near future. [48][49][50][51][52] The application of laser induced fluorescence detection systems (LIF) coupled to capillary gel electrophoresis (CGE) has provided extremely low detection limits, 25,60 and offers an interesting alternative to gain precision and sensitivity in the quantification of PCR products from competitive and multiplex PCR reactions. [61][62][63] Additionally, the CDCE technique (Constant-Denaturant Capillary Electrophoresis) allows to minimise the size differences among the specific and competitor sequences because it admits the employment of competitors very similar to the specific sequence without necessity of restriction analysis.…”

Section: Qc-pcrmentioning

confidence: 99%

Detection of Genetically Modified Organisms in Foods by DNA Amplification Techniques

García‐Cañas

Cifuentes

González

2004

Critical Reviews in Food Science and Nutrition

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“…Fung and Yeung 20 have proposed the use of polyethylene oxide for the pre‐coating process, García‐Cañas et al . 21 used PVA, and Giovannoli et al . 22 evaluated hydroxypropyl cellulose.…”

Section: Introductionmentioning

confidence: 99%

Development of an entangled polymer solution for improved resolution in DNA typing by CE

et al. 2008

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A new sieving matrix consisting of a mixture of two commercial polymers with different chemical structures, PVP (Mw=1,000,000 g/mol) and hydroxyethyl cellulose (HEC, Mw=250,000 g/mol), has been successfully used for single-stranded DNA separation. This sieving matrix was optimized with a Doehlert design, a second-order model statistical design. A total concentration of 3.5% of polymer, which contains 20.4% of PVP relative to HEC, combines the superior coating capability of the PVP with the high efficiency of the HEC. The use of this new sieving matrix gives highly resolved and reproducible separations of single-stranded DNA ranging from 50 to 500 bp. The separation of these DNA fragments is accomplished in less than 30 min under 319 V/cm with efficiencies up to 4 million plates per meter (measured at 350 bp). More than 90 successive analyses were achieved without any deterioration of the separation performance. Repeatability values of resolution (given as %RSD) for the analyzed DNA fragments are 2.2% (measured with the couple 139/150 bp) and 5.3% (measured with the couple 490/500 bp). Moreover, this mixture has a low viscosity (388 cP), which permits easy filling of fused-silica capillaries. This new sieving matrix, which exhibits high sieving performance, good dynamic coating ability, and low viscosity, can be a useful alternative to other less easily synthesized sieving matrices and eliminates the need for precoating the capillary to eliminate electroosmosis.

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Highly reproducible capillary gel electrophoresis (CGE) of DNA fragments using uncoated columns. Detection of genetically modified maize by PCR-cGE

Cited by 40 publications

References 26 publications

Recent advances in the application of capillary electromigration methods for food analysis

Recent advances in the application of capillary electromigration methods for food analysis

Detection of Genetically Modified Organisms in Foods by DNA Amplification Techniques

Development of an entangled polymer solution for improved resolution in DNA typing by CE

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