Detection of Genetically Modified Organisms in Foods by DNA Amplification Techniques

Recent advances in the application of capillary electromigration methods for food analysis

“…In this sense, our group has demonstrated for the first time the possibilities of the combined use of PCR and capillary gel electrophoresis with laser induced fluorescence (CGE-LIF) to detect genetically modified organisms (transgenic foods) [4,5]. Recently, a new PCR-CGE-LIF approach to quantitatively determine the content of genetically modified Bt maize in foods was developed [95].…”

“…Several reviews have been published on the application of CE methods in food analysis in the last three years, which is a good demonstration of the possibilities of this technique in this particular field. Table 1 summarizes the different reviews published on this topic [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], including the aforementioned review by Frazier and Papadopoulou [1].…”

“Mark the Gene”: a Method for Nondestructive Introduction of Marker Sequences Inside the Gene Frame of Transgenes

“…There have been several reports on the transfer of the functional gene by homologous recombination (30), and active dissemination of catabolic plasmids by HGT has also been studied as a strategy for bioremediation (7,34,36). After the dissemination of the transgene by homologous recombination, the current PCR detection technique, which relies on the primer pair that is complementary to the transgene and its adjacent site (1,11), is not reliable anymore. However, our NS marker would still be effective even after the genetic crossing, since the introduction sites are inside the gene frame of the functional transgene.…”

Detection of microbial food contaminants and their products by capillary electromigration techniques

“…CE has emerged as a separation technique that provides fast and efficient separations with minimum consumption of sample and reagents, allowing automation. In food analysis, CE has sufficiently demonstrated its potential and versatility for solving analytical problems such as ensuring compliance with food and trade laws, adulteration, contamination, quality control, chemical composition of foods, etc [13][14][15][16][17][18][19][20]. It is noteworthy to mention the increasingly growing interest on the application of CE to the microbiological research field that is reflected by the high number of works published on this topic during the last decade.…”