Detection of microbial food contaminants and their products by capillary electromigration techniques

García‐Cañas, Virginia; Cifuentes, Alejandro

doi:10.1002/elps.200700253

Cited by 35 publications

(25 citation statements)

References 123 publications

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“…CE for the analysis of colloidal or nano‐particles ( e.g. polymers and inorganic particles, microbes, mammalian cells, cell subunits and other biological particles) has been fully reviewed 25, 26. It has many advantages over other analytical methods for analyzing submicron particles, including common availability, frit‐less operation, high mass sensitivity, high separation efficiency, minute volume of sample, small volume of buffer and measurement of effective mobility which represents the charge to size ratio ( q / r ) for a particle 27–30.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis characterization of molecularly imprinted polymer particles in fast binding with 17β‐estradiol

DeMaleki

Lai

Da̧bek-Złotorzyńska

2010

J of Separation Science

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Molecularly imprinted polymer (MIP) submicron particles were synthesized, using either ethylene glycol dimethacrylate or trimethylolpropane trimethacrylate as a cross-linker, specifically for recognition of 17β-estradiol (E2). HPLC with fluorescence detection (HPLC-FD) results showed that 90(±5)% of E2 bound onto these particles after 2 min of incubation, and 96(±3)% after long equilibrium. The binding capacity was 8(±3) μmol/g for MIP particles prepared using ethylene glycol dimethacrylate, and 33-43(±8) μmol/g for using trimethylolpropane trimethacrylate. CE separation of MIP and non-imprinted polymer particles was successful when 50 mM borate buffer (pH 8.5) containing 0.005% w/v EOTrol™ LN in reverse polarity (-30 kV) was used. The electrophoretic mobilities of MIP and non-imprinted polymer particles, together with dynamic light scattering measurement of particle sizes, allowed for an estimation of their surface charges. Automated injection of E2 and particles in mixture set a lower limit of 20(±1) s on incubation time for the study of fast binding kinetics. The presence of E2 and bisphenol A (BPA) together tested the selectivity of MIP particles, when the two compounds competed for available binding cavities or sites. Addition of E2 after BPA confirmed E2 occupation of the specific binding cavities, via displacement of BPA.

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Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis characterization of molecularly imprinted polymer particles in fast binding with 17β‐estradiol

DeMaleki

Lai

Da̧bek-Złotorzyńska

2010

J of Separation Science

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“…milk, kidney, fruit, wine, tea, fish, juice and chicken, among others). Although there are scientific articles showing the potential of electrophoretic methods using real samples [4,7,[16][17][18][19], unfortunately, it is not always possible to find papers in which the authors have demonstrated the potential of the CE methodologies using samples in which the analytes are in native form and consequently the interaction matrix-analyte is unpredictable. In most cases, spiked samples are used to demonstrate this relative potential.…”

Section: Ce Companies and Market Statusmentioning

confidence: 95%

Thirty years of capillary electrophoresis in food analysis laboratories: Potential applications

2011

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CE has generated considerable interest in the research community since instruments were introduced by different trading companies in the 1990s. Nowadays, CE is popular due to its simplicity, speed, highly efficient separations and minimal solvent and reagent consumption; it can also be included as a useful technique in the nanotechnology field and it covers a wide range of specific applications in different fields (chemical, pharmaceutical, genetic, clinical, food and environmental). CE has been very well evaluated in research laboratories for several years, and different new approaches to improve sensitivity (one of the main drawbacks of CE) and robustness have been proposed. However, this technique is still not well accepted in routine laboratories for food analysis. Researching in data bases, it is easy to find several electrophoretic methods to determine different groups of analytes and sometimes they are compared in terms of sensitivity, selectivity, precision and applicability with other separation techniques. Although these papers frequently prove the potential of this methodology in spiked samples, it is not common to find a discussion of the well-known complexity of the matrices to extract analytes from the sample and/or to study the interferences in the target analytes. Summarizing, the majority of CE scientific papers focus primarily on the effects upon the separation of the analytes while ignoring their behavior if these analytes are presented in real samples.

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“…The plot shows an upsurge trend indicative of the increased interest and activity. Sixty eight percent (68%) of these publications came out in 2006–2010, the coverage of this review, with the following topics: pharmaceutical and herbal products applications 16–49, food and food contaminants 50–88, detection 89–103, toxicological compounds 104–111, microchips 67, 88, 112, and preconcentration 113–120. There were reviews on the general advances and new aspects of CE‐natural products analysis 15, 121, 122.…”

Section: Previous Reviewsmentioning

confidence: 99%

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

2011

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An extensive search of the published research and review articles indicated a growing number of works that employed electroseparation techniques. Capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC), in particular, were found to be popular for the analyses of natural products. This review outlines the important developments in this field over the years, especially during the 2006-2010 period. An overview of the natural product applications such as pharmaceuticals/herbal products, fingerprinting and quality control, food and food contaminants, and toxicological compounds of interest to forensics were presented. Important areas of detection strategies, microchips, sample preconcentration, and chiral separations were also discussed. Hence, quick information was provided on the researches already undertaken and the possibilities of unexplored areas. In addition, fundamental concepts for the understanding of CZE and EKC and their suitability for natural products analyses were briefly discussed.

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Detection of microbial food contaminants and their products by capillary electromigration techniques

Cited by 35 publications

References 123 publications

Capillary electrophoresis characterization of molecularly imprinted polymer particles in fast binding with 17β‐estradiol

Capillary electrophoresis characterization of molecularly imprinted polymer particles in fast binding with 17β‐estradiol

Thirty years of capillary electrophoresis in food analysis laboratories: Potential applications

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

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