Highly efficient capture of circulating tumor cells with low background signals by using pyramidal microcavity array

Microfluidic-Based Technologies for CTC Isolation: A Review of 10 Years of Intense Efforts towards Liquid Biopsy

“…This separation method relies on cell size properties, as well as on a combination of size and deformability criteria. Several strategies were studied for CTC isolation: microstructure post filters [28][29][30][31][32], microporous membranes [33][34][35][36][37], or microfluidic constrictions [38][39][40][41][42][43].…”

“…Recently, another MCA structure was imagined by Yin et al, since vertical entrances (presented above) keep blood cells from entering and escaping the microcavities and thus decrease the efficiency and purity of separation [46]. They integrated an MCA filter with pyramidal microstructures into a microfluidic device for CTC enrichment from raw blood samples [34] (Figure 3C). The silicon-based microcavity array was fabricated by lithography and induced coupled plasma-reactive ion etching (ICPRIE) technologies to obtain 10,000 microcavities in a 14 × 14 mm filter.…”

“…R T and R L are, respectively, the radius of the curvature of the trailing and leading edges of the cell. Reprinted from [34], copyright 2019, with permission from Elsevier. (D) The Parsortix™ system.…”

Microfluidics for Biomedical Analysis

“…The CTC capture efficiency of designed micro filters exceeded 83%. By employing this device, those unwanted blood cells will deform and pass through the cavities with the overall contamination rate lower than 0.003% (Figure b) . Another example of microfluidics‐based single cell analysis is to use a microfluidic chip to capture single cancer cell via a trap for in situ coimmunoprecipitation analysis.…”

“…The modification of anti‐EpCAM antibodies onto the pyramidal microcavity filter improves the enrichment of circulating tumor cells. Reproduced with permission . Copyright 2019, Elsevier.…”