Microfluidics for Biomedical Analysis

Abstract

Many new techniques and materials have been applied to microfluidic systems in the past decade, and integrated microfluidic chips have become powerful platforms for realizing highly sensitive, high throughput, and low‐cost analysis. This article reviews the latest advancements of microfluidic platforms for biological and biomedical analysis in the fields of fundamental biology research, clinical application, food safety, and environmental monitoring, with a focus on nucleic acid‐based molecular diagnosis and protein‐based immunodiagnosis. The combination of microfluidics with advanced techniques (e.g., nanotechnology, 3D printing, deep learning) would offer innovative new tools for bioanalyses. The current challenges and the future developments of microfluidic technologies for clinical applications are also discussed. It is anticipated that this review will be beneficial to promoting microfluidics toward a broad range of applications in the future.

Universal and high-fidelity DNA single nucleotide polymorphism detection based on a CRISPR/Cas12a biochip

“…So we designed a centrifugal microuidic chip and the corresponding point-of-care analytical equipment to reduce the exogenous interference in the detection process. [26][27][28][29][30] Considering the complexity of mixing reagents and the difficulty of storing-crRNA solution, as in our previous reports, we preloaded the mixed reagents of the CRISPR/Cas12a system into the biochip by freeze-drying technology. 31 As shown in Fig.…”

Experimental Analysis of Laser Micromachining of Microchannels in Common Microfluidic Substrates

“…Microfluidics has become one of the fastest growing technologies that finds its application in various engineering, biomedical, chemistry, pharmaceutical, biologic, and forensic science applications [ 1 , 2 ]. Microfluidics devices offer numerous competitive advantages over conventional processes such as small physical footprint, reduced reagent consumption, rapid outcomes, and higher efficiency [ 3 , 4 ]. Performance and applications of microfluidic devices depend on the substrate materials and their properties [ 5 , 6 ].…”

“…Performance and applications of microfluidic devices depend on the substrate materials and their properties [ 5 , 6 ]. For instance, biological experiments with cells in microfluidics devices need a biocompatible substrate otherwise cells might not be able to survive and perform regular cellular functions [ 3 ]. On the other hand, chemical experiments in microfluidics require a chemically stable substrate that can withstand acid, alkali, solvent, and organic reactions [ 7 ].…”

Tunable Harmonic Flow Patterns in Microfluidic Systems through Simple Tube Oscillation

“…conditions. [1,2] Microfluidic systems are dominated by viscous forces, resulting in highly ordered, laminar flows that mitigate flow disturbances. [3,4] This improves their control and predictability, but negates many complex tasks that require the ability to generate customized disturbed flows.…”

“…Microfluidic systems facilitate the manipulation of liquids within miniaturized structures, which not only reduce the volume of samples but also offer unparalleled opportunities for performing complex, multistep chemical, biochemical, and biological reactions under highly controlled conditions. [ 1,2 ] Microfluidic systems are dominated by viscous forces, resulting in highly ordered, laminar flows that mitigate flow disturbances. [ 3,4 ] This improves their control and predictability, but negates many complex tasks that require the ability to generate customized disturbed flows.…”