An ultra-thin silicon nitride membrane for label-free CTCs isolation from whole blood with low WBC residue

Quan, Yunlin; Zhu, Zhongyi; Tang, Dezhi; Zhu, Shengli; Wang, Cailian; Chen, Ke; Zhang, Ni

doi:10.1016/j.seppur.2022.121349

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…However, this system suffers from high cost, poor specificity, and low separation efficiency in practical operation, which limits its clinical application [15,16]. Physical methods utilize physical fields like acoustic, electric, or magnetic fields [17][18][19][20][21] as well as filtration and hydrodynamic methods [22][23][24] to separate CTCs from blood based on their physical characteristics (such as size, density, dielectric properties, deformability). Electrical separation methods utilize the different dielectric properties of cancer cells and blood cells in an electric field, which are driven to deflect the cancer cells in different directions for the purpose of separating the cancer cells.…”

Section: Introductionmentioning

confidence: 99%

The Shape Effect of Acoustic Micropillar Array Chips in Flexible Label-Free Separation of Cancer Cells

Lin,

Zhu,

et al. 2024

Micromachines

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The precise isolation of circulating tumor cells (CTCs) from blood samples is a potent tool for cancer diagnosis and clinical prognosis. However, CTCs are present in extremely low quantities in the bloodstream, posing a significant challenge to their isolation. In this study, we propose a non-contact acoustic micropillar array (AMPA) chip based on acoustic streaming for the flexible, label-free capture of cancer cells. Three shapes of micropillar array chips (circular, rhombus, and square) were fabricated. The acoustic streaming characteristics generated by the vibration of microstructures of different shapes are studied in depth by combining simulation and experiment. The critical parameters (voltage and flow rate) of the device were systematically investigated using microparticle experiments to optimize capture performance. Subsequently, the capture efficiencies of the three micropillar structures were experimentally evaluated using mouse whole blood samples containing cancer cells. The experimental results revealed that the rhombus microstructure was selected as the optimal shape, demonstrating high capture efficiency (93%) and cell activity (96%). Moreover, the reversibility of the acoustic streaming was harnessed for the flexible release and capture of cancer cells, facilitating optical detection and analysis. This work holds promise for applications in monitoring cancer metastasis, bio-detection, and beyond.

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Section: Introductionmentioning

confidence: 99%

The Shape Effect of Acoustic Micropillar Array Chips in Flexible Label-Free Separation of Cancer Cells

Lin,

Zhu,

et al. 2024

Micromachines

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show abstract

“…Physical-based isolation [25,26] could have approximately 90%-100% capture efficiency, and high throughput could be acquired especially with unique circular spiral design [27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

“…Small-sized CTCs, low EpCAM-expressed CTCs, and mensenchymal-expressed CTCs tend to ow away. Physicalbased isolation 25,26 could achieve an approximately 90-100% capture efficiency, and a high throughput could be acquired too, especially with a unique circular spiral design. [27][28][29][30] Sarioglu et al presented a chip with triangular microposts arranged in parallel.…”

Section: Introductionmentioning

confidence: 99%