Highly Aligned Polymer Nanofiber Structures: Fabrication and Applications in Tissue Engineering

Beachley, Vince; Katsanevakis, Eleni; Zhang, Ning; Wen, Xuejun

doi:10.1007/12_2011_141

Cited by 42 publications

(44 citation statements)

References 186 publications

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“…This can be seen in Figure 7(a), where cells are preferentially growing out from the sphere along the fibers, whereas cells on a flat surface (Figure 7(b)) do not show this behavior and outgrowth is randomly orientated. Cell alignment along orientated electrospun fibers has been shown previously for different cell types, 79,80 and Lim et.al observed similar behavior as we do for adult neural stem cells. 81 negative cell migration, seen by DAPI staining, out from the neurosphere for spheres on electrospun fiber substrates.…”

Section: Cell Culture On Electrospun Fibers In a Microfluidic Channelsupporting

confidence: 90%

A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications

Wallin

Zandén²,

Carlberg³

et al. 2012

Biomicrofluidics

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The properties of a cell's microenvironment are one of the main driving forces in cellular fate processes and phenotype expression in vivo. The ability to create controlled cell microenvironments in vitro becomes increasingly important for studying or controlling phenotype expression in tissue engineering and drug discovery applications. This includes the capability to modify material surface properties within well-defined liquid environments in cell culture systems. One successful approach to mimic extra cellular matrix is with porous electrospun polymer fiber scaffolds, while microfluidic networks have been shown to efficiently generate spatially and temporally defined liquid microenvironments. Here, a method to integrate electrospun fibers with microfluidic networks was developed in order to form complex cell microenvironments with the capability to vary relevant parameters. Spatially defined regions of electrospun fibers of both aligned and random orientation were patterned on glass substrates that were irreversibly bonded to microfluidic networks produced in poly-dimethyl-siloxane. Concentration gradients obtained in the fiber containing channels were characterized experimentally and compared with values obtained by computational fluid dynamic simulations. Velocity and shear stress profiles, as well as vortex formation, were calculated to evaluate the influence of fiber pads on fluidic properties. The suitability of the system to support cell attachment and growth was demonstrated with a fibroblast cell line. The potential of the platform was further verified by a functional investigation of neural stem cell alignment in response to orientation of electrospun fibers versus a microfluidic generated chemoattractant gradient of stromal cell-derived factor 1 alpha. The described method is a competitive strategy to create complex microenvironments in vitro that allow detailed studies on the interplay of topography, substrate surface properties, and soluble microenvironment on cellular fate processes. V C 2012 American Institute of Physics. [http://dx

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Section: Cell Culture On Electrospun Fibers In a Microfluidic Channelsupporting

confidence: 90%

A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications

Wallin

Zandén²,

Carlberg³

et al. 2012

Biomicrofluidics

View full text Add to dashboard Cite

show abstract

“…Scaffold designed for periodontal regeneration tissue also requires that cells seeded in vitro or recruited in vivo are allowed to infiltrate and populate the scaffolds quickly [41]. To this end, we simulated the process that adjacent cells infiltrated and proliferate into the scaffolds from the cross-sectioned areas of 3D scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Incorporation of aligned PCL–PEG nanofibers into porous chitosan scaffolds improved the orientation of collagen fibers in regenerated periodontium

et al. 2015

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“…10 On such aligned substrates, stem cells are arranged in a direction parallel to the nanofibers' alignment. 8 Moreover, increased calcium and collagen production was observed in stem cells cultured on aligned nanofibers as opposed to randomly oriented scaffolds 11 because cytoskeletal elongation altered by aligned nanofibers may activate certain osteo-related gene expression via mechanotransduction. 11 Given the superior performance in bone formation, highly ordered nanofibers are considered a promising candidate of scaffolds for bone tissue engineering.…”

Section: Gao Et Almentioning

confidence: 99%

“…8 Moreover, increased calcium and collagen production was observed in stem cells cultured on aligned nanofibers as opposed to randomly oriented scaffolds 11 because cytoskeletal elongation altered by aligned nanofibers may activate certain osteo-related gene expression via mechanotransduction. 11 Given the superior performance in bone formation, highly ordered nanofibers are considered a promising candidate of scaffolds for bone tissue engineering. 12 It has been gradually recognized that among various parameters that influence cell-material interactions, not only topographical but also biochemical cues should be considered in the design of scaffold materials that can mimic the chemical and physical features of microenvironments in native tissues.…”

Section: Gao Et Almentioning

confidence: 99%

Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering

Gao¹,

Zhang²,

Song³

et al. 2015

IJN

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The construction of functional biomimetic scaffolds that recapitulate the topographical and biochemical features of bone tissue extracellular matrix is now of topical interest in bone tissue engineering. In this study, a novel surface-functionalized electrospun polycaprolactone (PCL) nanofiber scaffold with highly ordered structure was developed to simulate the critical features of native bone tissue via a single step of catechol chemistry. Specially, under slightly alkaline aqueous solution, polydopamine (pDA) was coated on the surface of aligned PCL nanofibers after electrospinning, followed by covalent immobilization of bone morphogenetic protein-7-derived peptides onto the pDA-coated nanofiber surface. Contact angle measurement, Raman spectroscopy, and X-ray photoelectron spectroscopy confirmed the presence of pDA and peptides on PCL nanofiber surface. Our results demonstrated that surface modification with osteoinductive peptides could improve cytocompatibility of nanofibers in terms of cell adhesion, spreading, and proliferation. Most importantly, Alizarin Red S staining, quantitative real-time polymerase chain reaction, immunostaining, and Western blot revealed that human mesenchymal stem cells cultured on aligned nanofibers with osteoinductive peptides exhibited enhanced osteogenic differentiation potential than cells on randomly oriented nanofibers. Furthermore, the aligned nanofibers with osteoinductive peptides could direct osteogenic differentiation of human mesenchymal stem cells even in the absence of osteoinducting factors, suggesting superior osteogenic efficacy of biomimetic design that combines the advantages of osteoinductive peptide signal and highly ordered nanofibers on cell fate decision. The presented peptide-decorated bone-mimic nanofiber scaffolds hold a promising potential in the context of bone tissue engineering.

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Highly Aligned Polymer Nanofiber Structures: Fabrication and Applications in Tissue Engineering

Cited by 42 publications

References 186 publications

A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications

A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications

Incorporation of aligned PCL–PEG nanofibers into porous chitosan scaffolds improved the orientation of collagen fibers in regenerated periodontium

Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering

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