Incorporation of aligned PCL–PEG nanofibers into porous chitosan scaffolds improved the orientation of collagen fibers in regenerated periodontium

Jiang, Wenlu; Li, Long; Zhang, Ding; Huang, Shishu; Zheng, Jian; Wu, Yeke; Zhao, Zhihe; Zhao, Lixing; Zhou, Shaobing

doi:10.1016/j.actbio.2015.07.023

Cited by 107 publications

(95 citation statements)

References 60 publications

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“…Among them are natural and synthetic polymers, glasses, ceramics and hydrogels . Furthermore, a great many methods have been proposed to improve these materials, for example by altering their structural properties or by preloading them with growth or differentiation factors . As stated above, the choice of material depends on the specific tissue‐engineering goal.…”

Section: Leads For Tissue Engineering Of the Pdlmentioning

confidence: 99%

The intricate anatomy of the periodontal ligament and its development: Lessons for periodontal regeneration

Jong

Bakker

Everts

et al. 2017

J of Periodontal Research

151

137

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The periodontal ligament (PDL) connects the tooth root and alveolar bone. It is an aligned fibrous network that is interposed between, and anchored to, both mineralized surfaces. Periodontal disease is common and reduces the ability of the PDL to act as a shock absorber, a barrier for pathogens and a sensor of mastication. Although disease progression can be stopped, current therapies do not primarily focus on tissue regeneration. Functional regeneration of PDL may be achieved using innovative techniques, such as tissue engineering. However, the complex fibrillar architecture of the PDL, essential to withstand high forces, makes PDL tissue engineering very challenging. This challenge may be met by studying PDL anatomy and development. Understanding PDL anatomy, development and maintenance provides clues regarding the specific events that need to be mimicked for the formation of this intricate tissue. Owing to the specific composition of the PDL, which develops by self-organization, a different approach than the typical combination of biomaterials, growth factors and regenerative cells is necessary for functional PDL engineering. Most specifically, the architecture of the new PDL to be formed does not need to be dictated by textured biomaterials but can emerge from the local mechanical loading conditions. Elastic hydrogels are optimal to fill the space properly between tooth and bone, may house cells and growth factors to enhance regeneration and allow self-optimization by the alignment to local stresses. We suggest that cells and materials should be placed in a proper mechanical environment to initiate a process of self-organization resulting in a functional architecture of the PDL.

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Section: Leads For Tissue Engineering Of the Pdlmentioning

confidence: 99%

The intricate anatomy of the periodontal ligament and its development: Lessons for periodontal regeneration

Jong

Bakker

Everts

et al. 2017

J of Periodontal Research

151

137

View full text Add to dashboard Cite

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“…Jiang et al reported increased PDL-like tissue formation with mature collagen fibers using aligned PCL-polyethylene glycol (PEG) nanofibers embedded into porous chitosan scaffolds and seeded with bone marrow mesenchymal stem cells. [21] Similar findings have been observed in applications for neural and cardiac tissue regeneration: Koepsell et al noted increased cellular orientation of intervertebral disc-derived cells on increasingly aligned electrospun PCL fibers resulted in higher ECM production, including collagen and glycosaminoglycans, [22] while Ifkovits et al found enhanced collagen alignment on oriented poly(glycerol sebacate) fibers when seeded with cardiocytes and implanted subcutaneously. [23] However, it remains difficult to create three-dimensional scaffolds using these techniques without the presence of a more complex and customizable system such as a 3-D printer that could position fibers in directions relevant to the anatomical structure of tissue we attempt to model, thereby recapitulating its unique geometric complexity.…”

Section: Discussionmentioning

confidence: 56%

Integration of 3D Printed and Micropatterned Polycaprolactone Scaffolds for Guidance of Oriented Collagenous Tissue Formation In Vivo

Pilipchuk

Monje

Jiao

et al. 2016

Adv Healthcare Materials

100

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Scaffold design incorporating multi-scale cues for clinically-relevant, aligned tissue regeneration has potential to improve structural and functional integrity of multi-tissue interfaces. The objective of this pre-clinical study was to develop poly(ε-caprolactone) (PCL) scaffolds with mesoscale and microscale architectural cues specific to human ligament progenitor cells and assess their ability to form aligned bone-ligament-cementum complexes in vivo. PCL scaffolds were designed to integrate a 3D printed bone region with a micropatterned PCL thin film consisting of grooved pillars. The patterned film region was seeded with human ligament cells, fibroblasts transduced with BMP-7 genes seeded within the bone region, and a tooth dentin segment positioned on the ligament region prior to subcutaneous implantation into a murine model. Results indicated increased tissue alignment in vivo using micropatterned PCL films, compared to random-porous PCL. At 6 weeks, 30um groove depth significantly enhanced oriented collagen fiber thickness, overall cell alignment, and nuclear elongation relative to 10um groove depth. This study demonstrates for the first time that scaffolds with combined hierarchical mesoscale and microscale features can align cells in vivo for oral tissue repair with potential for improving the regenerative response of other bone-ligament complexes.

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“…Moreover, 19.7% of the studies evaluated CS in the regeneration of skin [24, 27, 29, 63–71], 14.7% in nervous tissue [30, 72–79], 11.5% in cartilage [32, 81–84, 87, 88], and 3.3% in periodontal [33, 90] structures. The remaining studies involved regeneration of colorectal [26], mammary [89], tympanic membrane [31], and vascular [34] tissues (1.6% each).…”

Section: Resultsmentioning

confidence: 99%

Versatility of Chitosan-Based Biomaterials and Their Use as Scaffolds for Tissue Regeneration

Ribeiro

Vieira

Melo

et al. 2017

The Scientific World Journal

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Chitosan is a naturally occurring polysaccharide obtained from chitin, present in abundance in the exoskeletons of crustaceans and insects. It has aroused great interest as a biomaterial for tissue engineering on account of its biocompatibility and biodegradation and its affinity for biomolecules. A significant number of research groups have investigated the application of chitosan as scaffolds for tissue regeneration. However, there is a wide variability in terms of physicochemical characteristics of chitosan used in some studies and its combinations with other biomaterials, making it difficult to compare results and standardize its properties. The current systematic review of literature on the use of chitosan for tissue regeneration consisted of a study of 478 articles in the PubMed database, which resulted, after applying inclusion criteria, in the selection of 61 catalogued, critically analysed works. The results demonstrated the effectiveness of chitosan-based biomaterials in 93.4% of the studies reviewed, whether or not combined with cells and growth factors, in the regeneration of various types of tissues in animals. However, the absence of clinical studies in humans, the inadequate experimental designs, and the lack of information concerning chitosan's characteristics limit the reproducibility and relevance of studies and the clinical applicability of chitosan.

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Incorporation of aligned PCL–PEG nanofibers into porous chitosan scaffolds improved the orientation of collagen fibers in regenerated periodontium

Cited by 107 publications

References 60 publications

The intricate anatomy of the periodontal ligament and its development: Lessons for periodontal regeneration

The intricate anatomy of the periodontal ligament and its development: Lessons for periodontal regeneration

Integration of 3D Printed and Micropatterned Polycaprolactone Scaffolds for Guidance of Oriented Collagenous Tissue Formation In Vivo

Versatility of Chitosan-Based Biomaterials and Their Use as Scaffolds for Tissue Regeneration

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