Higher prevalence of FTO gene risk genotypes AA rs9939609, CC rs1421085, and GG rs17817449 and saliva containing Staphylococcus aureus in obese women in Croatia

Huđek, Ana; Škara, Lucija; Smolković, Barbara; Kazazić, Snježana; Ravlić, Sandra; Nanić, Lucia; Osvatić, Martina Matovinović; Jelčić, Jozo; Rubelj, Ivica; Bačun-Družina, Višnja

doi:10.1016/j.nutres.2017.12.005

Cited by 17 publications

(14 citation statements)

References 44 publications

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“…This is similar to another study that reported the presence of C . kefyr in oral microbiota only in obese subjects compared to normal‐weight subjects 52 . However, other studies related the presence of C .…”

Section: Discussionmentioning

confidence: 94%

The intestinal mycobiota and its relationship with overweight, obesity and nutritional aspects

García‐Gamboa

Kirchmayr

Gradilla‐Hernández

et al. 2021

J Human Nutrition Diet

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Background The fungal community of the gastrointestinal tract has recently become of interest, and knowledge of its relationship with the development of obesity is scarce. The present study aimed to evaluate the cultivable fungal fraction from the microbiota and to analyze its relationship with obesity. Methods Samples were taken from 99 participants with normal weight, overweight and obesity (n = 31, 34 and 34, respectively) and were cultivated in selective medium, and the cultivable yeasts were identified by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. Anthropometric and biochemical measures were also evaluated. Results Eutrophic, overweight and obese groups presented concentrations of 1.6, 2.16 and 2.19 log10 colony‐forming units g–1 yeast, respectively. Ascomycota and Basidiomycota were the two identified phyla. At the genus level, Candida spp. showed a relatively high prevalence, and 10 different species were detected: Candida glabrata, Candida orthopsilosis, Candida lambica, Candida kefyr, Candida albicans, Candida krusei, Candida valida, Candida parapsilosis, Candida utilis and Candida humilis (with relative abundances of 71.72%, 5.05%, 21.21%, 6.06%, 29.29%, 27.27%, 8.08%, 16.16%, 1.01% and 2.02%, respectively). Conclusions The obese group presented a higher prevalence of Candida albicans. Furthermore, Candida albicans, Candida kefyr and Rhodotorula mucilaginosa showed a high positive correlation with obesity, weight gain and fat mass and showed a negative correlation with high‐density lipoprotein and lean mass, parameters related to weight loss.

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Section: Discussionmentioning

confidence: 94%

The intestinal mycobiota and its relationship with overweight, obesity and nutritional aspects

García‐Gamboa

Kirchmayr

Gradilla‐Hernández

et al. 2021

J Human Nutrition Diet

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“…17 Similarly, these results are supported by those of Huđek et al, who found a statistically significant positive correlation between frequencies of high-risk FTO genotypes in obese women (AA rs9939609, CC rs1421085 and GG rs17817449). 22 Díaz-Anzaldúa et al reported that differences in mean BMI levels among Mexican patients with bipolar disorder were explained by the presence of FTO rs8050136 and rs9939609 genotypes. 23 Another study showed that the locus of the FTO gene was significantly associated with increased BMI in indigenous Mexican populations.…”

Section: Discussionmentioning

confidence: 99%

Correlation of the Homeostasis Model Assessment Index and Adiponectin, Leptin and Insulin Levels to Body Mass Index-Associated Gene Polymorphisms in Adolescents

Martinez-Martinez

Mendieta-Zerón

Celis

et al. 2018

Sultan Qaboos Univ Med J

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Objectives: This study aimed to describe correlations between glucose, insulin and adipokine levels and the homeostasis model assessment (HOMA) index with regards to the presence/absence of fat mass and obesity-associated (FTO) rs9939609 and peroxisome proliferator-activated receptor (PPAR)-y rs1801282 single nucleotide polymorphisms (SNPs) as indicators of body mass index in adolescents. Methods: This cross-sectional study was conducted between September and December 2016 in Toluca, Mexico. A total of 71 students between 14–18 years old were included. Various anthropometric and laboratory measurements were collected, including lipid profile, glucose, insulin and adipokine levels and HOMA index. The degree of association between variables was evaluated with regards to the presence/absence of the SNPs. Results: Leptin levels were significantly higher among female students (P = 0.001), although adiponectin levels did not differ significantly (P = 0.060). There were significant positive correlations between insulin levels and HOMA index with FTO (r = 0.391; P = 0.007 and r = 0.413; P = 0.005, respectively) and PPARγ (r = 0.529; P = 0.007 and r = 0.537; P = 0.007, respectively) SNPs. Leptin showed a significant positive correlation in the presence of PPARγ (r = 0.483; P = 0.007) or in the absence of both SNPs (r = 0.627; P = 0.039). However, adiponectin was significantly negatively correlated in the presence of FTO, either alone (r = −0.333; P = 0.024) or in combination with PPARγ (r = −0.616; P = 0.043). Conclusion: The presence of FTO and/or PPARγ SNPs might be related to a genetic predisposition to metabolic syndrome.Keywords: Obesity; Body Mass Index; Single Nucleotide Polymorphisms; Fat Mass and Obesity Associated Protein, Human; Peroxisome Proliferator-Activated Receptor gamma; Adipokines.

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“…The 25-hydroxy vitamin D, B 12 and folic acid concentrations were determined in the serum of fresh pooled blood samples. Furthermore, after rinsing the mouth with 15 mL of sterile saline solution to prevent the effect of previously eaten food on the microorganisms in the oral cavity, 2 mL of saliva samples were collected using sterilized centrifuge tubes (TPP, Trasadingen, Switzerland) and stored at −20 °C ( 20 ).…”

Section: Methodsmentioning

confidence: 99%

“…The isolation and identification procedures were previously described by Huđek et al ( 20 ). Original saliva and a 1:1 dilution of saliva (0.5 mL) in phosphate buffer (pH=7.2) were inoculated on nutrient agar plates (de Man-Rogosa-Sharpe and Luria-Bertani).…”

Section: Methodsmentioning

confidence: 99%

Association of Vitamins D, B9 and B12 with Obesity-Related Diseases and Oral Microbiota Composition in Obese Women in Croatia

Turković

Matovinović

Žuna

et al. 2022

Food Technol. Biotechnol. (Online)

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Research background. Oral microbiota has become an important factor in obesity, but its association with obesity-related diseases and serum 25-hydroxy vitamin D [25(OH)D] and B complex levels is still uncertain. The main aim of the paper was to determine variation in oral microbiota composition regarding vitamin status and obesity-related diseases in obese females from Croatia. We hypothesized that the prevalence of probiotic or pathogen bacteria in the oral cavity of obese women in Croatia depends on vitamin B9 (folic acid), B12, and 25(OH)D serum levels and/or hypertension, diabetes, and prediabetes diagnosis. Experimental approach. To test the defined research hypothesis, female individuals with body mass index (BMI) ?30 kg/m2 (N=70) were recruited to participate in this study. Obese women were divided into groups according to BMI value, diagnosis of obesity-related diseases, and micronutrients blood levels. For the quantitative determination of folic acid, vitamin B12, and 25(OH)D serum levels, an electrochemiluminescence protein binding assay (ECLIA) was performed. Isolated microorganisms from the saliva of obese women were analyzed by MALDI-TOF mass spectrometer. Results and conclusions. The present results do not support the hypothesis that the prevalence of probiotic or pathogen bacteria in the oral cavity of obese women in Croatia depends on the level of micronutrients in obese women from Croatia. On the other hand, hypertension and diabetes/prediabetes diagnosis favor the growth of oral pathogens, specifically increased levels of Candida sp. Novelty and scientific contribution. To the best of our knowledge, this is the first study showing the relationship between obesity, micronutrient level, oral microbiota composition, and the incidence of obesity-related disease. We included only obese women from Croatia, so it is regionally specific. Also, we have shown that oral microbiota composition is not connected with micronutrient deficiencies but only with obesity-related diseases.

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Higher prevalence of FTO gene risk genotypes AA rs9939609, CC rs1421085, and GG rs17817449 and saliva containing Staphylococcus aureus in obese women in Croatia

Cited by 17 publications

References 44 publications

The intestinal mycobiota and its relationship with overweight, obesity and nutritional aspects

The intestinal mycobiota and its relationship with overweight, obesity and nutritional aspects

Correlation of the Homeostasis Model Assessment Index and Adiponectin, Leptin and Insulin Levels to Body Mass Index-Associated Gene Polymorphisms in Adolescents

Association of Vitamins D, B9 and B12 with Obesity-Related Diseases and Oral Microbiota Composition in Obese Women in Croatia

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