High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica

Abstract

An acetate-negative mutant of Yarrowia lipolytica Wratislavia K1 was selected that, when grown with 300 g raw glycerol l(-1) at pH 3, produced 170 g erythritol l(-1) after 7 days, corresponding to a 56% yield and a productivity of 1 g l(-1) h(-1). The Wratislavia K1 strain did not produce citric acid.

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

“…The application of crude glycerol at 13.3 Hz and 0.6 min -1 resulted in erythritol concentration of 58.2 g/L, yield of 0.38 g/g and productivity of 0.78 g/(L·h). On the other hand, Rymowicz et al (9) reported that in the fed-batch cultivation the application of crude glycerol (300 g/L) with Y. lipolytica Wratislavia K1 resulted in a higher concentration of erythritol (170 g/L) with the same yield of erythritol production of 0.56 g/g and lower productivity of 1.0 g/(L·h) than in the present study. Bett er results were obtained by Tomaszewska et al (13) when using the parental strain Y. lipolytica Wratislavia K1 in the fed-batch system with pulsed addition of glycerol (325 g/L).…”

“…Batch or fed-batch cultures on glycerol are commonly used as systems for erythritol production, and therefore glycerol seems to be an effi cient substrate for this purpose (9,14). As reported before, in batch culture the same strain, Y. lipolytica MK1, was able to produce up to 82.2 g/L of erythritol from glycerol with yield of 0.55 g/g and productivity of 0.84 g/(L·h) (19).…”

“…(7) or Pseudozyma tsukubaensis (8). Moreover, erythritol may be produced at low pH values also from glycerol by the oleaginous yeast Yarrowia lipolytica (9,10). Various factors aff ecting fermentation have been examined, e.g.…”

“…Various factors aff ecting fermentation have been examined, e.g. diff erent cultivation systems, type of substrate, and addition of salt or supplementation with minerals (9,(11)(12)(13)(14)(15). Up to now, the batch, fed-batch or repeated--batch cultures have been tested for erythritol biosynthesis from glycerol (9,13).…”

Biorefinery for Glycerol Rich Biodiesel Industry Waste

“…Y. lipolytica strain Wratislavia K1 co-produced citric acid (87-89 g/L) and erythritol (47 g/L) [112]. An interesting feature of strain K1 was its ability to selectively produce erythritol from CG [113]. Higher (71 g/L) citric acid yields from CG have also been reported [114].…”