High yield formation of lipid bilayer shells around silicon nanowires in aqueous solution

Römhildt, Lotta; Gang, A.; Baraban, Larysa; Opitz, Joerg; Cuniberti, Gianaurelio

doi:10.1088/0957-4484/24/35/355601

Cited by 7 publications

(7 citation statements)

References 41 publications

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“…These curved regions can be uniquely valuable in systematic studies of the influence of curvature on biomolecules at lipid membranes. Further, while it has been shown that lipid bilayers form on silicon nanowire field effect transistors, , as well as on a single silicon nanowire laid horizontally and nanowires dispersed in solution, the possibility to use arrays of vertical nanowires–bilayer hybrid structures opens up the capabilities to study interactions at the membrane interface using a range of surface sensitive techniques. Unlike dispersed nanowires, the nanowires here are vertically aligned and attached to a surface, a feature that will potentially enable us to localize and track interactions taking place at any individual nanowire.…”

mentioning

confidence: 99%

Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays

et al. 2014

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Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to form continuous and locally highly curved model membranes. Distinct from standard flat supported lipid bilayers, the high aspect ratio of the nanowires results in a large bilayer surface available for the immobilization and study of biomolecules. We used these bilayers to bind a membrane-anchored protein as well as tethered vesicles on the nanowire substrate. The nanowire-bilayer platform shown here can be expanded from fundamental studies of lipid membranes on controlled curvature substrates to the development of innovative membrane-based nanosensors.

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confidence: 99%

Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays

et al. 2014

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“…The fluorescence intensity inside the photobleached area was quantified using ImageJ in order to generate fluorescence recovery curves. The half-time of recovery ( t 1/2 ) was obtained from the fitting of the obtained curves (and then the diffusion coefficient ( D ) was estimated using the following equation: D = (0.88 × w 2 )/4 t 1/2 , where w indicates the radius of the bleached area). , …”

Section: Methodsmentioning

confidence: 99%

NTA-Cholesterol Analogue for the Nongenetic Liquid-Ordered Phase-Specific Functionalization of Lipid Membranes with Proteins

et al. 2023

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The nongenetic modification of cell membranes with proteins is a straightforward way of cellular engineering. In these processes, it is important to specifically address the proteins to liquid-ordered (Lo) or liquid-disordered (Ld) domains as this can largely affect their biological functions. Herein, we report a cholesterol analogue (CHIM) with a nitrilotriacetic acid (NTA) headgroup, named CHIM-NTA. CHIM-NTA integrates into lipid membranes similar to the widely used phospholipid-derived DGS–NTA and, when loaded with Ni2+, allows for specific membrane immobilization of any polyhistidine-tagged proteins of choice. Yet, unlike DGS–NTA, it localizes to the Lo phase in phase-separated giant unilamellar vesicles (GUVs) and allows addressing His-tagged proteins to Lo domains. Furthermore, CHIM-NTA readily integrates into the membranes of live cells and thus enables the nongenetic modification of the cell surface with proteins. Overall, CHIM-NTA provides a facile and flexible way to modify biological membranes, in particular Lo domains, with His-tagged proteins and can serve as a broadly applicable molecular tool for cell surface engineering.

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“…Since cell membranes play a critical role in cellular interactions, studies have recently been carried out to encapsulate NWs in a lipid bilayer as to mimic a cell membrane [9][10][11][12]. The electrical detection of ion transport through pore channels in a lipid bilayer coated Si NW was recently demonstrated in [13], revealing the potential of using Si NW devices as artificial cell constructs.…”

Section: Introductionmentioning

confidence: 99%

“…While the in-situ formation of lipid bilayers on Si NWs was studied using fluorescence microscopy in [12], real-time electrical measurements performed to analyze the temporal effect of the membrane formation process on NW conductivity were absent. A change in the electrical resistance for a bilayer coated vs. uncoated NW was demonstrated only ex-situ by Misra et al [13] and Martinez et al [10].…”

Section: Introductionmentioning

confidence: 99%

Real-time electrical detection of the formation and destruction of lipid bilayers on silicon nanowire devices

Williams

Juba

et al. 2015

Sensing and Bio-Sensing Research

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a b s t r a c tSilicon nanowire (Si NW) two-terminal devices were fabricated to electrically probe the real-time formation and destruction of lipid bilayers. A liposome solution, containing the same ratio of zwitterionic/anionic lipids that are present in an Escherichia coli cell membrane, was applied to the NW devices. Lipid bilayer formation on the Si NWs was detected in-situ by observing electrical resistance changes complemented by confocal fluorescence microscopy imaging. The formation of lipid bilayers resulted in a 1% to 2% decrease in device current, consistent with the negative gating effect of the lipids on the NW surface. The devices demonstrated a % 1 min electrical response time to lipid encapsulation. Removal of the lipid layer was achieved by exposing the devices to a detergent, which resulted in NW conductance returning to its original value with a % 2 min recovery time. The lipid bilayer coated Si NWs demonstrate a novel platform to enable in-situ electrical probing of bacterial cell membrane mechanisms, interactions, and reactions.

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High yield formation of lipid bilayer shells around silicon nanowires in aqueous solution

Cited by 7 publications

References 41 publications

Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays

Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays

NTA-Cholesterol Analogue for the Nongenetic Liquid-Ordered Phase-Specific Functionalization of Lipid Membranes with Proteins

Real-time electrical detection of the formation and destruction of lipid bilayers on silicon nanowire devices

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