High throughput volatile fatty acid skin metabolite profiling by thermal desorption secondary electrospray ionisation mass spectrometry

Martin, Helmut; Reynolds, James C.; Riazanskaia, Svetlana; Thomas, C. L. Paul

doi:10.1039/c4an00134f

Cited by 29 publications

(26 citation statements)

References 42 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the term SESI is here restricted to the ionization of vapors. Coupled with Atmospheric Pressure Interface Mass Spectrometers (API-MS), it has been successfully used detect various types of analytes including Page 3 of 29 A c c e p t e d M a n u s c r i p t 3 explosives [17], chemical warfare agents simulants [18], drugs [19], human metabolites [20][21][22][23], food [24,25] and bacterial emissions [26,27].…”

Section: Introductionmentioning

confidence: 99%

Numerical modeling and experimental validation of a universal secondary electrospray ionization source for mass spectrometric gas analysis in real-time

Barrios-Collado

Vidal-de-Miguel

Sinués

2016

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

The process by which ambient vapors are ionized upon interaction with electrosprays is not fully understood, compromising its optimization and widespread use. In this work we evaluated the different scales associated with the processes involved in secondary electrospray ionization (SESI), and developed a new numerical method that merges the analytical solution that describes the angle of aperture and the current of an ideal electrospray, with a Finite Element Method that enables the evaluation of complex geometries. The numerical method showed that, despite the low ionization efficiency (i.e. ion concentration/neutral vapor concentration ~ 10 -4 ), depletion of neutral vapors plays an important role. We used this method to optimize and design a Low Flow SESI source, which was coupled with a commercial high resolution/high mass accuracy mass spectrometer. The system was designed to be interfaced with virtually any pre-existing atmospheric pressure ionization mass spectrometer. The experimental validation for the detection of ambient vapors confirmed qualitatively the numerical predictions in terms of ionization efficiency as a function of sample flow rate. As a result of the optimization, this prototype showed a 5 fold sensitivity increase against standard SESI.This novel add-on is meant to upgrade mass spectrometers to analyze trace gases in real time by SESI technique.

show abstract

Section: Introductionmentioning

confidence: 99%

Numerical modeling and experimental validation of a universal secondary electrospray ionization source for mass spectrometric gas analysis in real-time

Barrios-Collado

Vidal-de-Miguel

Sinués

2016

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

show abstract

“…However, the compounds detected in axillary secretions are often technique dependent (Curran et al 2005, 2010; Dormont et al 2013; Martin et al 2014; Mebazaa et al 2011; Natsch et al 2004; Penn et al 2007; Preti et al 2006; Riazanskaia et al 2008; Zeng et al 1991, 1992, 1996). A review of these studies demonstrates that the characteristic axillary odorants are not generally seen using headspace sampling (e.g., thermal desorption or SPME) but are typically detected when solvent extraction techniques are employed.…”

Section: Discussionmentioning

confidence: 99%

Ethnic/Racial and Genetic Influences on Cerumen Odorant Profiles

et al. 2014

View full text Add to dashboard Cite

This report describes the volatile organic compounds (VOCs) associated with human cerumen (earwax) and the effects of ethnicity/race and variation on the ATP-binding cassette, sub-family C, member 11 gene (ABCC11). A single nucleotide polymorphism (SNP) in ABCC11 affects the cerumen VOC profiles of individuals from African, Caucasian, and Asian descent. Employing gas chromatography/mass spectrometry (GC/MS) we have identified the nature and relative abundance of cerumen VOCs from 32 male donors. Our results show that cerumen contains a complex mixture of VOCs and that the amounts of these compounds vary across individuals as well as across ethnic/racial groups. In six of the seven compounds whose detected concentrations were found to be statistically different across groups, individuals of African descent (AfD) > Caucasian descent (CaD) > Asians descent (AsD). Our findings also reveal that ABCC11 genotype alone does not predict the type and relative levels of volatiles found in human cerumen, and suggest that other biochemical pathways must be involved. Examination of the composition and diversity of external auditory canal microbiota in a small subset of our subject population revealed that the ear microbiota may not be directly correlated with either ethnic group membership or ABCC11 genotype.

show abstract

“…employed PDMS patches placed onto the axilla area of female participants for VOC sampling. The study aimed to investigate skin metabolites responsible for body odor with an emphasis on the effect of a single nucleotide polymorphism 538G → A in the ABCC11 gene on the concentrations of apocrine derived axillary odor molecules, especially 3-methy-2-hexenoic acid [36]. The investigation was concluded with four volatile fatty acid (VFA) target compounds being identified with limits of detection approximately around 100 s pg cm −2 , with authors noting that a typical skin patch has an area of 0.5 cm 2 .…”

Section: Polydimethylsiloxane (Pdms)mentioning

confidence: 99%

Advancements in Non-Invasive Biological Surface Sampling and Emerging Applications

Nalbant

Boyacı

2019

Separations

View full text Add to dashboard Cite

Biological surfaces such as skin and ocular surface provide a plethora of information about the underlying biological activity of living organisms. However, they pose unique problems arising from their innate complexity, constant exposure of the surface to the surrounding elements, and the general requirement of any sampling method to be as minimally invasive as possible. Therefore, it is challenging but also rewarding to develop novel analytical tools that are suitable for in vivo and in situ sampling from biological surfaces. In this context, wearable extraction devices including passive samplers, extractive patches, and different microextraction technologies come forward as versatile, low-invasive, fast, and reliable sampling and sample preparation tools that are applicable for in vivo and in situ sampling. This review aims to address recent developments in non-invasive in vivo and in situ sampling methods from biological surfaces that introduce new ways and improve upon existing ones. Directions for the development of future technology and potential areas of applications such as clinical, bioanalytical, and doping analyses will also be discussed. These advancements include various types of passive samplers, hydrogels, and polydimethylsiloxane (PDMS) patches/microarrays, and other wearable extraction devices used mainly in skin sampling, among other novel techniques developed for ocular surface and oral tissue/fluid sampling.

show abstract

High throughput volatile fatty acid skin metabolite profiling by thermal desorption secondary electrospray ionisation mass spectrometry

Abstract: Global VOC skin metabolite profiling. Thermal desorption secondary electrospray ionisation time-of-flight mass spectrometry classifies skin odour phenotypes by targeted volatile fatty analysis. Examination of the mass spectra reveals the potential for global metabolic studies.

Cited by 29 publications

References 42 publications

Numerical modeling and experimental validation of a universal secondary electrospray ionization source for mass spectrometric gas analysis in real-time

Numerical modeling and experimental validation of a universal secondary electrospray ionization source for mass spectrometric gas analysis in real-time

Ethnic/Racial and Genetic Influences on Cerumen Odorant Profiles

Advancements in Non-Invasive Biological Surface Sampling and Emerging Applications

Contact Info

Product

Resources

About