High-throughput selected reaction monitoring liquid chromatography–mass spectrometry determination of methylphenidate and its major metabolite, ritalinic acid, in rat plasma employing monolithic columns

Barbarin, Nicolas; Mawhinney, Douglas B.; Black, Roderick S.; Henion, Jack D.

doi:10.1016/s1570-0232(02)00487-7

Cited by 51 publications

(30 citation statements)

References 22 publications

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“…A high-throughput LC-MS/MS method using a Chromolith RP-18 monolithic column was developed for the determination of bupropion, an antidepressant drug, and its metabolites, hydrobupropion and threo-hydropupropion in human, mouse, and rat plasma (Borges et al, 2004). The monolithic column performance as a function of a column backpressure, peak asymmetry, and retention time reproducibility are adquately maintained over 864 extracted plasma injections like as a high-througput LC-MS/MS method for the determination of methylphenidate (MPH), a central nervous system stimulant, and its de-esterified metabolite, ritalinic acid (RA) in rat plasma samples (Barbarin et al, 2003). A monolithic www.intechopen.com Tandem Mass Spectrometry -Applications and Principles 464 column was directly compared to a conventional C18 column as the analytical column in method validation of a drug and its epimer metabilite (Huang et al, 2006).…”

Section: Monolithic Chromatographymentioning

confidence: 99%

Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples

Kang¹

2012

Tandem Mass Spectrometry - Applications and Principles

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Section: Monolithic Chromatographymentioning

confidence: 99%

Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples

Kang¹

2012

Tandem Mass Spectrometry - Applications and Principles

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“…In fact, the development of selective reaction monitoring (SRM) was largely pioneered by those interested in detecting these types of compounds in highly complex and typically unfractionated samples [46][47][48][49][50][51]. The use of SRM for the robust detection of a multitude of compounds has been reported in human plasma [49,[52][53][54] and other complex human samples [55,56].…”

Section: Targeted Msmentioning

confidence: 99%

Quantitative strategies to fuel the merger of discovery and hypothesis-driven shotgun proteomics

Kline¹,

Finney²,

Wu³

2009

Briefings in Functional Genomics and Proteomics

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The ultimate goal of most shotgun proteomic pipelines is the discovery of novel biomarkers to direct the development of quantitative diagnostics for the detection and treatment of disease. Differential comparisons of biological samples identify candidate peptides that can serve as proxys of candidate proteins. While these discovery approaches are robust and fairly comprehensive, they have relatively low throughput. When merged with targeted mass spectrometry, this pipeline can fuel hypothesis-driven studies and the development of novel diagnostics and therapeutics.

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“…Another useful approach of assessing matrix effect is postcolumn infusion of an analyte into the MS detector. While the analyte is continuously infused through a tee to the mobile phase eluent, the extracted blank matrix is injected by an autosampler onto the analytical column [130][131][132][133][134][135][136]. The instrument configu-ration for post-column infusion experiment is shown in (Fig.…”

Section: Matrix Effects and Recoverymentioning

confidence: 99%

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

Zhou¹,

Song²,

Tang³

et al. 2005

CPA

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Swift growth in the use of LC-MS/MS for the analysis of drugs in biological matrices has been compelled by the need for timely and high-quality data at many stages in drug discovery and development process: from high throughput screening of drug candidates and rapid data generation for pre-clinical studies to almost 'real-time' analysis of clinical samples. Prompt and rational method development, validation, and transfer play a pivotal role in achieving the goals of "faster, better, and cheaper" for pharmacokinetic studies since this could easily account for more than 50% of the time and labor resources for a moderate-sized project. Strategy for rational method development, validation and transfer has been largely kept as institutional knowledge but rarely appeared in literature. In this review article, strategies for developing and validating robust high throughput LC-MS/MS methods will be critically reviewed and discussed. Automated sample preparation, fast chromatography, minimization of matrix effects, and strategy of narrowing the gap between validation and incurred sample analysis are just a few topics covered in this review. Other interesting approaches for improving method efficiency and ruggedness such as direct injection SPE and liquid/liquid extracts as well as multiplexing of LC columns will also be discussed. Potential pitfalls during method development and validation are pointed out. At the end, the question "how fast is fast enough and how fast is too fast?" will be answered after considering all aspects of the method development and validation.

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High-throughput selected reaction monitoring liquid chromatography–mass spectrometry determination of methylphenidate and its major metabolite, ritalinic acid, in rat plasma employing monolithic columns

Cited by 51 publications

References 22 publications

Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples

Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples

Quantitative strategies to fuel the merger of discovery and hypothesis-driven shotgun proteomics

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

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