High-Throughput Quantitative Screening of Peroxidase-Mimicking DNAzymes on a Microarray by Using Electrochemical Detection

Kaneko, Naoto; Horii, Katsunori; Kato, Shintaro; Akitomi, Joe; Waga, Iwao

doi:10.1021/ac4002518

Cited by 16 publications

(13 citation statements)

References 20 publications

(53 reference statements)

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“…Twelve melamine aptamers modified from the original poly-T melamine aptamer [ 18 ], and a designed peroxidase-mimicking DNAzyme [ 19 ], were used for screening ( Table 1 ). The original poly-T aptamer dimerized and bound to melamine.…”

Section: Methodsmentioning

confidence: 99%

An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

Kaneko¹,

Horii²,

Akitomi³

et al. 2018

Sensors

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Melamine, a nitrogen-rich compound, has been used as a food and milk additive to falsely increase the protein content. However, melamine is toxic, and high melamine levels in food or in milk can cause kidney and urinary problems, or even death. Hence, the detection of melamine in food and milk is desirable, for which numerous detection methods have been developed. Several methods have successfully detected melamine in raw milk; however, they require a sample preparation before the analyses. This study aimed to develop an aptamer-DNAzyme conjugated biosensor for label-free detection of melamine, in raw milk, without any sample preparation. An aptamer-DNAzyme conjugated biosensor was developed via screening using microarray analysis to identify the candidate aptamers followed by an optimization, to reduce the background noise and improve the aptamer properties, thereby, enhancing the signal-to-noise (S/N) ratio of the screened biosensor. The developed biosensor was evaluated via colorimetric detection and tested with raw milk without any sample preparation, using N-methylmesoporphyrin IX for fluorescence detection. The biosensor displayed significantly higher signal intensity at 2 mM melamine (S/N ratio, 20.2), which was sufficient to detect melamine at high concentrations, in raw milk.

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Section: Methodsmentioning

confidence: 99%

An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

Kaneko¹,

Horii²,

Akitomi³

et al. 2018

Sensors

Self Cite

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“…Since the first report on using hemin/G4 DNAzyme as the electrocatalytic label for amplifying sensing events [102], this approach attracted increasing interest in biosensor [103,104] and biofuel cell technologies [105]. In comparison with protein peroxidases, the hemin/G4 peroxidase-mimicking DNAzymes have several advantages, such as high chemical stability, low cost and simple synthesis.…”

Section: Hemin/g4 Dnazyme Electrochemical Biosensormentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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“…Hemin/G-quadruplex peroxidase-mimicking DNAzymes, which consist in G-quadruplex sequences with intercalated hemin molecules, have recently attract great interest in biosensor [98,99] and biofuel cell technologies [100]. Hemin is an iron- [111] or metal ions [112,113]).…”

Section: Hemin/g-quadruplex Dnazymes Electrochemical Biosensorsmentioning

confidence: 99%

Redox behaviour of G-quadruplexes

Chiorcea-Paquim

Oliveira-Brett

2014

Electrochimica Acta

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Please cite this article as: A.-M. Chiorcea-Paquim, A.M. OliveiraBrett, Redox behaviour of G-quadruplexes, Electrochimica Acta (2013), http://dx.doi.org/10.1016/j.electacta. 2013.07.150 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 45A c c e p t e d M a n u s c r i p t Redox behaviour of G-quadruplexes

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High-Throughput Quantitative Screening of Peroxidase-Mimicking DNAzymes on a Microarray by Using Electrochemical Detection

Cited by 16 publications

References 20 publications

An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

Guanine Quadruplex Electrochemical Aptasensors

Redox behaviour of G-quadruplexes

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