High-throughput detection of immobilized plasmonic nanoparticles by a hyperspectral imaging system based on Fourier transform spectrometry

Abstract

To facilitate the application of plasmonic nanoparticles (PNPs) in high-throughput detection, we develop a hyperspectral imaging system (HSIS) combining dark-filed microscopy and imaging Fourier transform spectrometry to measure scattering spectra from immobilized PNPs. The current setup has acquisition time of 5 seconds and spectral resolution of 21.4 nm at 532.1 nm. We demonstrate the applicability of the HSIS in conjunction with spectral data analysis to quantify multiple types of PNPs and detect small changes in localized surface plasmon resonance wavelengths of PNPs due to changes in the environmental refractive index.

Quantification of the optical properties of two-layered turbid media by simultaneously analyzing the spectral and spatial information of steady-state diffuse reflectance spectroscopy

“…The proximal end of the fiber bundle was positioned at the focal plane of the objective for the acquisition of hyperspectral images of the sample via the fiber bundle. characteristics of the hyperspectral imaging system have been published [29]. Briefly, a hyperspectral imaging data cube was obtained by capturing a series of images at regularly spaced optical path-length differences between two beams in an interferometer, recording the interference intensity as a function of the optical path-length difference for each pixel (interferogram), and Fourier transforming the interferograms pixel by pixel.…”

Review of spectral imaging technology in biomedical engineering: achievements and challenges

“…Another generally used spectral dispersion element is the interferometer, which can obtain spectral images by changing optical path-length difference (OPD) between two beams, recording the interference intensity as a function of the OPD (interferogram), and Fourier transforming the interferograms pixel by pixel. 19 For example, the Michelson interferometer and the Sagnac interferometer have been used to develop some spectral imaging systems for different biomedical research. 20,21 The detector is used to acquire the light intensity required to measure the spectrum at each pixel in the image.…”

“…181 Some high-photon collection efficiency or high-throughput spectral imaging systems also have been developed for working with the weak, incoherent sources common to biomedical applications. 19,28,183 These studies show that there are generally some ways to compensate for the photon collection efficiency, such as the use of a higher-quantum efficiency detector and a high-power light source to increase the exposure time. However, long exposure time may not be appropriate in applications where the scene is changing on a time scale of the acquisition.…”

Spatially Resolved Spectroscopic Characterization of Nanostructured Films by Hyperspectral Dark-Field Microscopy

“…Hyperspectral dark-field microscope (HSDFM) is a newly emerging multifunctional analytical tool that can simultaneously perform high-resolution single-wavelength imaging and high-resolution single-pixel spectroscopy for nanostructured metallic and dielectric films as well as biosamples. , According to the recently published papers on HSDFM, − its typical applications include monitoring the morphological evolution of nanoparticles in different biological environments, studying the cellular uptake of different nanoparticles, , detection of the targeted nanoparticles to cancer cells . Particularly, Tseng and El-Khoury research groups investigated the plasmonic characteristics of a single noble metal nanoparticle using the hyperspectral imaging system combined with a dark-field microscope and Fourier transform spectrometry. , Wonner et al studied the electrochemical oxidation and dissolution process of individual silver nanoparticles based on the electrochemical method and the HSDFM purchased from CytoViva Inc . Although hyperspectral dark-field imaging has made progress in the research of nanomaterials, most of the reported work has focused on its use in monitoring plasmonic nanoparticles.…”