Quantification of the optical properties of two-layered turbid media by simultaneously analyzing the spectral and spatial information of steady-state diffuse reflectance spectroscopy

Tseng, Te-Yu; Chen, Chun-Yu; Li, Yi-Shan; Sung, Kung-Bin

doi:10.1364/boe.2.000901

Cited by 53 publications

(27 citation statements)

References 35 publications

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“…The performance of this two-layer model may be witnessed by the stability of the recovered melanin content and epidermal thickness during the whole reactive hyperemia protocol. This two-layer model can be easily extended to multiple-layer systems and may also be applied beyond the skin in modeling other layered structures such as stratified epithelium and superficial stroma [38].…”

Section: Discussionmentioning

confidence: 99%

In vivo real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light

Chen

Lin

Wang

et al. 2017

Biomed. Opt. Express

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Abstract:We present the real-time single snapshot multiple frequency demodulation -spatial frequency domain imaging (SSMD-SFDI) platform implemented with a visible digital mirror device that is capable of imaging and monitoring dynamic turbid medium and processes over a large field of view. One challenge in quantitative imaging of biological tissue such as the skin is the complex structure rendering techniques based on homogeneous medium models to fail. To address this difficulty we have also developed a novel method that maps the layered structure to a homogeneous medium for spatial frequency domain imaging. The varying penetration depth of spatially modulated light on its wavelength and modulation frequency is used to resolve the layered structure. The efficacy of the real-time SSMD-SFDI platform and this two-layer model is demonstrated by imaging forearms of 6 healthy subjects under the reactive hyperemia protocol. The results show that our approach not only successfully decouples light absorption by melanin from that by hemoglobin and yields accurate determination of cutaneous hemoglobin concentration and oxygen saturation, but also provides reliable estimation of the scattering properties, the melanin content and the epidermal thickness in real time. Potential applications of our system in imaging skin physiological and functional states, cancer screening, and microcirculation monitoring are discussed at the end.

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Section: Discussionmentioning

confidence: 99%

In vivo real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light

Chen

Lin

Wang

et al. 2017

Biomed. Opt. Express

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“…Some reports exist on the application of inverse photon transport models on hyperspectral images after image acquisition. 16,17 This paper presents a deterministic hyperspectral inverse modeling approach based on diffusion theory and spectral unmixing. These analytical methods are suitable for GPU implementation.…”

Section: Introductionmentioning

confidence: 99%

Estimation of skin optical parameters for real-time hyperspectral imaging applications

2014

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Abstract. Hyperspectral imaging combines high spectral and spatial resolution in one modality. This imaging technique is a promising tool for objective medical diagnostics. However, to be attractive in a clinical setting, the technique needs to be fast and accurate. Hyperspectral imaging can be used to analyze tissue properties using spectroscopic methods, and is thus useful as a general purpose diagnostic tool. We combine an analytic diffusion model for photon transport with real-time analysis of the hyperspectral images. This is achieved by parallelizing the inverse photon transport model on a graphics processing unit to yield optical parameters from diffuse reflectance spectra. The validity of this approach was verified by Monte Carlo simulations. Hyperspectral images of human skin in the wavelength range 400-1000 nm, with a spectral resolution of 3.6 nm and 1600 pixels across the field of view (Hyspex VNIR-1600), were used to develop the presented approach. The implemented algorithm was found to output optical properties at a speed of 3.5 ms per line of image data. The presented method is thus capable of meeting the defined real-time requirement, which was 30 ms per line of data.The algorithm is a proof of principle, which will be further developed.

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“…Spatially resolved diffuse reflectance spectroscopy systems incorporate multiple detectors positioned at multiple distances with respect to the illumination source. Each detector selectively collects diffusely back-reflected photons penetrating to different depths within the tissue, thus enabling depth-resolved optical tissue characterization [2]. Typical SRDR probes are fiber-based and incorporate 3-8 source-detector pairs lacking dense measurement capability [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Spatially resolved diffuse reflectance spectroscopy of two-layer turbid media by densely packed multi-pixel photodiode reflectance probe

et al. 2016

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Spatially-resolved diffuse reflectance (SRDR) measurements provide photon path information, and enable layered tissue analysis. This paper presents experimental SRDR measurements on two-layer PDMS skin tissue-mimicking phantoms of varying top layer thicknesses, and bulk phantoms of varying optical properties using concentric multi-pixel photodiode array (CMPA) probes, and corresponding forward Monte Carlo simulations. The CMPA is the most densely packed semiconductor SRDR probe reported to date. Signal contrasts between the single layer phantom and bi-layer phantoms with varying top layer thicknesses are as high as 80%. The mean error between the Monte Carlo simulations and the experiment is less than 6.2 %.

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Quantification of the optical properties of two-layered turbid media by simultaneously analyzing the spectral and spatial information of steady-state diffuse reflectance spectroscopy

Cited by 53 publications

References 35 publications

In vivo real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light

In vivo real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light

Estimation of skin optical parameters for real-time hyperspectral imaging applications

Spatially resolved diffuse reflectance spectroscopy of two-layer turbid media by densely packed multi-pixel photodiode reflectance probe

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