High-throughput behavioral screening method for detecting auditory response defects in zebrafish

Bang, Pascal I.; Yelick, Pamela C.; Malicki, Jarema; Sewell, William F.

doi:10.1016/s0165-0270(02)00118-8

Cited by 101 publications

(52 citation statements)

References 42 publications

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“…Previous studies investigating the hearing system of zebrafish of the AB strain demonstrated that they were able to respond behaviorally to an acoustic stimulus after 5 d.p.f. from frequencies of 200 Hz upwards (Bang et al, 2002;Zeddies and Fay, 2005). We determined that in this particular set of experiments that 113 dB of 200 Hz gave the optimum measurable response.…”

Section: Discussionmentioning

confidence: 99%

“…By 5 days post fertilization (d.p.f. ), free-swimming zebrafish larvae have developed morphological structures for hearing that allow them to perform an ASR when exposed to acoustic stimuli (Bang et al, 2002;Zeddies and Fay, 2005). Since most compound screens are usually performed in rodents, the zebrafish may offer an alternative and unique approach to identifying novel therapeutics; the startle response being used as a measure of information processing and attentional deficits.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Non-Associative Learning in Larval Zebrafish

Best¹,

Berghmans²,

Hunt³

et al. 2007

Neuropsychopharmacol

210

150

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Habituation, where a response is reduced when exposed to a continuous stimulus is one of the simplest forms of non-associative learning and has been shown in a number of organisms from sea slugs to rodents. However, very little has been reported in the zebrafish, a model that is gaining popularity for high-throughput compound screens. Furthermore, since most of the studies involving learning and memory in zebrafish have been conducted in adults, we sought to determine if zebrafish larvae could display non-associative learning and whether it could be modulated by compounds identified in previous rodent studies. We demonstrated that zebrafish larvae (7 days post fertilization) exhibit iterative reduction in a startle response to a series of acoustic stimuli. Furthermore, this reduction satisfied criteria for habituation: spontaneous recovery, more rapid reductions in startle to shorter intertrial intervals and dishabituation. We then investigated the pathways mediating this behavior using established compounds in learning and memory. Administration of rolipram (PDE4 inhibitor), donepezil (acetylcholinesterase inhibitor), and memantine (N-methyl-D-aspartic acid (NMDA) receptor antagonist) all increased the acoustic startle response and decreased habituation in the larvae, similar to previous rodent studies. Further studies demonstrated that NMDA blocked the memantine response and the effect of donepezil was blocked by mecamylamine but not atropine suggesting that the donepezil response was mediated by nicotinic rather than muscarinic receptors. Zebrafish larvae possess numerous advantages for medium to high-throughput screening; the model described herein therefore offers the potential to screen for additional compounds for further study on cognition function.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Non-Associative Learning in Larval Zebrafish

Best¹,

Berghmans²,

Hunt³

et al. 2007

Neuropsychopharmacol

210

150

View full text Add to dashboard Cite

show abstract

“…The most commonly used behavioral measure of auditory function in larval zebrafish is the startle response (Bang et al, 2000;Bang et al, 2002). It is an innate, reliable and robust behavior elicited by fast, high intensity stimuli.…”

Section: Introductionmentioning

confidence: 99%

Auditory sensitivity of larval zebrafish (Danio rerio) measured using a behavioral prepulse inhibition assay

Bhandiwad

Zeddies²,

Raible

et al. 2013

Journal of Experimental Biology

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SUMMARYZebrafish (Danio rerio) have become a valuable model for investigating the molecular genetics and development of the inner ear in vertebrates. In this study, we employed a prepulse inhibition (PPI) paradigm to assess hearing in larval wild-type (AB) zebrafish during early development at 5-6days post-fertilization (d.p.f.). We measured the PPI of the acoustic startle response in zebrafish using a 1-dimensional shaker that simulated the particle motion component of sound along the fish's dorsoventral axis. The thresholds to startle-inducing stimuli were determined in 5-6d.p.f. zebrafish, and their hearing sensitivity was then characterized using the thresholds of prepulse tone stimuli (90-1200Hz) that inhibited the acoustic startle response to a reliable startle stimulus (820Hz at 20dB re. 1ms). Hearing thresholds were defined as the minimum prepulse tone level required to significantly reduce the startle response probability compared with the baseline (no-prepulse) condition. Larval zebrafish showed greatest auditory sensitivity from 90 to 310Hz with corresponding mean thresholds of −19 to −10dB re. 1ms -2 , respectively. Hearing thresholds of prepulse tones were considerably lower than previously predicted by startle response assays. The PPI assay was also used to investigate the relative contribution of the lateral line to the detection of acoustic stimuli. After aminoglycoside-induced neuromast hair-cell ablation, we found no difference in PPI thresholds between treated and control fish. We propose that this PPI assay can be used to screen for novel zebrafish hearing mutants and to investigate the ontogeny of hearing in zebrafish and other fishes.

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“…Transgenic lines expressing fluorescent proteins in specific neuronal subpopulations have also been developed, which could facilitate screening. Numerous functional and behavioral assays, including assays of vision, hearing, touch responsiveness, memory, anxiety, and startle habituation have been developed and could also be useful for identifying neurotoxicants that do not cause obvious developmental phenotypes (Brockerhoff et al, 1995;Bang et al, 2002;Fetcho et al, 1998;Peitsaro et al, 2003). It is possible that a panel of several high-throughput morphological and functional assays could be used to screen broadly for neurotoxicants.…”

Section: Potential Applications For Zebrafish In Neurotoxicology (Rtmentioning

confidence: 99%

Use of non-mammalian alternative models for neurotoxicological study

et al. 2008

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The field of neurotoxicology needs to satisfy two opposing demands: the testing of a growing list of chemicals, and resource limitations and ethical concerns associated with testing using traditional mammalian species. National and international government agencies have defined a need to reduce, refine or replace mammalian species in toxicological testing with alternative testing methods and non-mammalian models. Toxicological assays using alternative animal models may relieve some of this pressure by allowing testing of more compounds while reducing expense and using fewer mammals. Recent advances in genetic technologies and the strong conservation between human and non-mammalian genomes allows for the dissection of the molecular pathways involved in neurotoxicological responses and neurological diseases using genetically tractable organisms. In this review, applications of four non-mammalian species, Zebrafish, cockroach, Drosophila, and Caenorhabditis elegans, in the investigation of neurotoxicology and neurological diseases are presented.

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High-throughput behavioral screening method for detecting auditory response defects in zebrafish

Cited by 101 publications

References 42 publications

Non-Associative Learning in Larval Zebrafish

Non-Associative Learning in Larval Zebrafish

Auditory sensitivity of larval zebrafish (Danio rerio) measured using a behavioral prepulse inhibition assay

Use of non-mammalian alternative models for neurotoxicological study

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